Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RCG00_RS03505 Genome accession   NZ_CP133217
Coordinates   510545..511702 (-) Length   385 a.a.
NCBI ID   WP_308872075.1    Uniprot ID   A0AA51MQJ4
Organism   Thiothrix subterranea strain DNT52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505545..516702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG00_RS03485 (RCG00_03485) - 506462..507955 (+) 1494 WP_308872070.1 NADH-quinone oxidoreductase subunit M -
  RCG00_RS03490 (RCG00_03490) - 507958..508476 (-) 519 WP_308872071.1 DUF1566 domain-containing protein -
  RCG00_RS03495 (RCG00_03495) bioD 508762..509421 (+) 660 WP_308872073.1 dethiobiotin synthase -
  RCG00_RS03500 (RCG00_03500) - 509418..510548 (-) 1131 WP_308872074.1 PilT/PilU family type 4a pilus ATPase -
  RCG00_RS03505 (RCG00_03505) pilU 510545..511702 (-) 1158 WP_308872075.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCG00_RS03510 (RCG00_03510) pilT 511716..512750 (-) 1035 WP_308135872.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG00_RS03515 (RCG00_03515) - 512812..513498 (+) 687 WP_308872077.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCG00_RS03520 (RCG00_03520) - 513505..514002 (-) 498 WP_308872079.1 DUF4124 domain-containing protein -
  RCG00_RS03525 (RCG00_03525) - 514334..515227 (+) 894 WP_308135869.1 (Fe-S)-binding protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42994.61 Da        Isoelectric Point: 7.5373

>NTDB_id=872519 RCG00_RS03505 WP_308872075.1 510545..511702(-) (pilU) [Thiothrix subterranea strain DNT52]
MDRDAAVSYVHKLLATMLEKKASDLYVTAEAAPSAKIASEMVPLTNQPLNEQTARMLVRAIMNDRQLKQFEEDMECNFSI
SLPGKARFRVNAFTQRGCAGMVLRHIPNHIPGLKDLGLPPVLRDIAMTKRGLVIMVGATGSGKSTTLASMVDFRNTNSKG
HIVTIEDPIEFVHQHKGCLITQREVGVDTSSYEIAMKNTLRQAPDVILLGEIRDREAMDYAIAYAETGHLCLSTLHANSS
NQAIDRIINFFPEERRNQLLMDLSLNLKAVVSQRLVRKAKGEGRLPAVEVVINTPLMADLILKGDVPGMKVLTSKSREHG
MRTFDQALFDLIEARQITVHEALRHADSQNDLRLRLKLESRFAQDNDFFKGLDELAIEPLENATR

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=872519 RCG00_RS03505 WP_308872075.1 510545..511702(-) (pilU) [Thiothrix subterranea strain DNT52]
ATGGATCGCGATGCTGCTGTTAGCTACGTGCATAAGTTATTGGCGACTATGTTGGAAAAAAAGGCTTCCGACTTGTACGT
TACAGCGGAGGCAGCGCCTTCTGCCAAGATTGCCAGCGAAATGGTGCCGTTAACCAATCAGCCATTGAATGAGCAAACGG
CGCGGATGTTGGTGCGGGCGATTATGAATGACCGCCAGCTCAAACAGTTCGAGGAGGACATGGAATGCAACTTTTCCATT
AGTCTACCTGGCAAAGCGCGATTCCGGGTAAACGCTTTCACGCAACGCGGTTGTGCGGGGATGGTCTTGCGCCATATTCC
TAACCATATTCCGGGTTTGAAGGATTTGGGTTTGCCGCCGGTATTGCGCGATATTGCCATGACCAAGCGCGGTTTGGTGA
TTATGGTGGGAGCGACCGGTTCGGGTAAATCGACTACATTGGCGTCGATGGTCGATTTCCGCAATACCAATTCCAAAGGG
CATATTGTCACGATTGAAGACCCGATTGAGTTTGTGCATCAGCACAAAGGGTGCTTGATTACGCAGCGCGAAGTGGGGGT
CGATACCAGCAGTTATGAAATTGCGATGAAAAATACCTTGCGCCAAGCCCCTGATGTGATTTTGCTGGGGGAAATTCGGG
ATCGCGAGGCGATGGATTACGCGATTGCTTACGCTGAAACCGGGCATTTGTGCTTGTCGACCTTGCACGCCAACAGTTCC
AACCAAGCGATTGACCGTATTATCAACTTTTTCCCGGAAGAACGCCGCAACCAGTTATTGATGGATTTGTCCCTCAATTT
GAAGGCGGTGGTATCGCAGCGCTTGGTACGCAAAGCCAAAGGGGAAGGGCGTTTGCCAGCAGTGGAGGTGGTGATTAATA
CCCCATTAATGGCGGATTTGATCCTCAAGGGCGATGTGCCGGGCATGAAAGTGCTGACCTCAAAATCGCGCGAACACGGG
ATGCGCACCTTCGACCAAGCCTTGTTTGACTTGATTGAAGCGCGGCAAATCACCGTGCATGAAGCGTTGCGTCATGCCGA
TTCGCAGAATGACTTGCGCTTACGCCTCAAATTGGAAAGCCGGTTTGCGCAAGATAATGACTTCTTTAAGGGCTTGGATG
AGTTGGCGATTGAGCCGCTGGAGAATGCCACACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.312

90.649

0.538
  pilU Acinetobacter baylyi ADP1

56.484

90.13

0.509
  pilU Vibrio cholerae strain A1552

50.862

90.39

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

87.532

0.392
  pilT Acinetobacter baumannii D1279779

43.529

88.312

0.384
  pilT Acinetobacter baumannii strain A118

43.529

88.312

0.384
  pilT Acinetobacter nosocomialis M2

43.235

88.312

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.261

90.649

0.374
  pilT Vibrio cholerae strain A1552

41.261

90.649

0.374
  pilT Legionella pneumophila strain Lp02

43.243

86.494

0.374
  pilT Legionella pneumophila strain ERS1305867

43.243

86.494

0.374
  pilT Acinetobacter baylyi ADP1

43.963

83.896

0.369
  pilT Pseudomonas stutzeri DSM 10701

43.034

83.896

0.361