Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAM80_RS15450 Genome accession   NZ_CP133165
Coordinates   3317282..3318316 (-) Length   344 a.a.
NCBI ID   WP_416427347.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3312282..3323316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS15425 (RAM80_15395) - 3312447..3313451 (+) 1005 WP_416427342.1 aspartate carbamoyltransferase catalytic subunit -
  RAM80_RS15430 (RAM80_15400) - 3313448..3314719 (+) 1272 WP_416427343.1 dihydroorotase -
  RAM80_RS15435 (RAM80_15405) - 3314771..3315190 (-) 420 WP_416427344.1 NINE protein -
  RAM80_RS15440 (RAM80_15410) - 3315440..3316126 (+) 687 WP_416427345.1 C40 family peptidase -
  RAM80_RS15445 (RAM80_15415) pilU 3316128..3317258 (-) 1131 WP_416427346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAM80_RS15450 (RAM80_15420) pilT 3317282..3318316 (-) 1035 WP_416427347.1 type IV pilus twitching motility protein PilT Machinery gene
  RAM80_RS15455 (RAM80_15425) - 3318383..3319072 (+) 690 WP_416427349.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAM80_RS15460 (RAM80_15430) proC 3319107..3319925 (+) 819 WP_416427350.1 pyrroline-5-carboxylate reductase -
  RAM80_RS15465 (RAM80_15435) - 3319935..3320525 (+) 591 WP_416427351.1 YggT family protein -
  RAM80_RS15470 (RAM80_15440) metX 3320658..3321797 (+) 1140 WP_416427352.1 homoserine O-succinyltransferase MetX -
  RAM80_RS15475 (RAM80_15445) metW 3321805..3322425 (+) 621 WP_416427353.1 methionine biosynthesis protein MetW -
  RAM80_RS15480 (RAM80_15450) - 3322450..3322887 (+) 438 WP_416427354.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37999.70 Da        Isoelectric Point: 6.7273

>NTDB_id=872255 RAM80_RS15450 WP_416427347.1 3317282..3318316(-) (pilT) [Pseudomonas sp. App30]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDKQRKDFEEYLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFRKITDVPRGLVLVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSNALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGALGMQTLDMCL
KDLVARGLIGREAAREKAKSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=872255 RAM80_RS15450 WP_416427347.1 3317282..3318316(-) (pilT) [Pseudomonas sp. App30]
ATGGACATTACTGAATTGCTGGCTTTCAGCGCCAAGCAAGGCGCTTCCGACCTGCACCTGTCCGCCGGGCTGCCGCCAAT
GATCCGGGTCGATGGTGATGTACGCAGAATCAATTTGCCAGCACTGGATCACAAGCAGGTCCACGAGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAGTACCTGGAGACTGACTTCTCCTTCGAAGTACCTGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCTGGCGCAGTGTTCCGTACCATCCCCTCCAAGGTGCTGACCATGGA
AGACCTGGGCATGGGCGATGTGTTTCGAAAGATTACCGATGTGCCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CGGGCAAGTCCACCACGCTGGCAGCGATGATCGACTACCTGAACAACAACAAACACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCTCCAA
CGCCCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCGGCCGAGGAGAAATCCATGGTCCGCTCCATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGTGGCCGGGTGGCGGCGCACGAAATCATGATCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGACCTGGTGGCCCGCGGGCTGATCGGTCGCGAGGCGGCGCGCGAGAAGGCCAAGTCCCCCGACAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Pseudomonas aeruginosa PAK

92.733

100

0.927
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.739

100

0.419
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404