Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RAM80_RS15445 Genome accession   NZ_CP133165
Coordinates   3316128..3317258 (-) Length   376 a.a.
NCBI ID   WP_416427346.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311128..3322258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS15415 (RAM80_15385) ruvX 3311428..3311868 (+) 441 WP_416427340.1 Holliday junction resolvase RuvX -
  RAM80_RS15420 (RAM80_15390) pyrR 3311923..3312435 (+) 513 WP_416427341.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  RAM80_RS15425 (RAM80_15395) - 3312447..3313451 (+) 1005 WP_416427342.1 aspartate carbamoyltransferase catalytic subunit -
  RAM80_RS15430 (RAM80_15400) - 3313448..3314719 (+) 1272 WP_416427343.1 dihydroorotase -
  RAM80_RS15435 (RAM80_15405) - 3314771..3315190 (-) 420 WP_416427344.1 NINE protein -
  RAM80_RS15440 (RAM80_15410) - 3315440..3316126 (+) 687 WP_416427345.1 C40 family peptidase -
  RAM80_RS15445 (RAM80_15415) pilU 3316128..3317258 (-) 1131 WP_416427346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAM80_RS15450 (RAM80_15420) pilT 3317282..3318316 (-) 1035 WP_416427347.1 type IV pilus twitching motility protein PilT Machinery gene
  RAM80_RS15455 (RAM80_15425) - 3318383..3319072 (+) 690 WP_416427349.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAM80_RS15460 (RAM80_15430) proC 3319107..3319925 (+) 819 WP_416427350.1 pyrroline-5-carboxylate reductase -
  RAM80_RS15465 (RAM80_15435) - 3319935..3320525 (+) 591 WP_416427351.1 YggT family protein -
  RAM80_RS15470 (RAM80_15440) metX 3320658..3321797 (+) 1140 WP_416427352.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41904.36 Da        Isoelectric Point: 7.5392

>NTDB_id=872254 RAM80_RS15445 WP_416427346.1 3316128..3317258(-) (pilU) [Pseudomonas sp. App30]
MEFERLLKLMVDKAGSDLFITPGLPPSLKVHGKIMPVTKNAMSPEQVREAVLGLMSEPQRLAFVEHRECNFAIHARDIGR
FRVSAFCQRSLPAMVIRRIESRIPTFDELKLPDTLKGLAMLKRGLVLVVGATGTGKSSTLAAMIDHRSKHSSGHIITIED
PIEFLHEHQNCIVTQREVGIDTDSFEVALRNTLRQAPDVIMIGEVRSRETMGQALSFAETGHLCVATLHASNADQALERV
ISFFPQDRHTQVWMELSLNLRAIIAQQLVPTPDGTSRRAVVEILVNTPLMADRIRRGEVPGLKTLMRRTNDQGMRTLDQA
LYELYREKAISYEDALAYADSANDLRLMIKLESSTGAEPREGLNLAPERGPTPGSR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=872254 RAM80_RS15445 WP_416427346.1 3316128..3317258(-) (pilU) [Pseudomonas sp. App30]
ATGGAGTTCGAAAGGCTGTTGAAACTGATGGTCGACAAGGCCGGTTCCGACCTTTTCATCACGCCAGGCCTGCCACCCTC
GCTGAAGGTGCACGGCAAGATCATGCCGGTCACCAAGAACGCCATGAGCCCCGAGCAGGTGCGCGAGGCCGTGCTGGGGC
TGATGAGCGAGCCCCAGCGGCTGGCGTTCGTCGAGCACCGCGAGTGCAACTTCGCCATCCACGCCCGCGACATCGGCCGC
TTCCGCGTCAGCGCGTTCTGCCAGCGCAGCCTGCCGGCCATGGTCATCCGCCGCATCGAAAGCCGCATTCCCACCTTCGA
TGAGCTGAAACTGCCCGACACCCTCAAGGGCCTGGCCATGCTCAAGCGCGGCCTGGTGCTGGTAGTGGGCGCCACTGGCA
CCGGCAAGTCCTCGACCCTGGCGGCGATGATCGACCACCGCAGCAAGCACAGCAGCGGCCACATCATCACCATCGAGGAC
CCCATCGAGTTCCTCCACGAACACCAGAACTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGACTCGTTCGAAGT
GGCACTGCGCAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAGGTGCGCAGCCGCGAGACCATGGGCCAGG
CCCTGAGCTTCGCCGAAACCGGCCACCTGTGCGTGGCCACCCTGCATGCCTCCAACGCCGACCAGGCGCTGGAGCGGGTG
ATCAGCTTCTTCCCCCAGGACCGCCACACCCAAGTGTGGATGGAGCTGTCGCTGAACCTGCGCGCCATCATCGCCCAGCA
ACTGGTGCCCACCCCGGATGGAACTAGTCGGCGTGCCGTGGTCGAAATCCTGGTGAATACGCCGCTGATGGCCGACCGCA
TCCGCCGGGGCGAGGTGCCGGGCCTGAAAACCCTGATGCGCCGCACCAACGACCAGGGCATGCGTACCCTGGACCAGGCG
TTGTACGAGTTGTACCGGGAGAAGGCGATCAGCTACGAGGACGCGCTGGCGTATGCCGATTCGGCGAACGACCTGCGGTT
GATGATCAAGCTGGAGTCGAGCACGGGCGCGGAGCCCAGGGAGGGGTTGAACCTGGCGCCGGAACGGGGCCCGACGCCAG
GGTCACGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

72.346

95.213

0.689
  pilU Acinetobacter baylyi ADP1

58.575

100

0.59
  pilU Vibrio cholerae strain A1552

56.857

93.085

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.437

97.34

0.394