Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q9R17_RS12920 Genome accession   NZ_CP133160
Coordinates   2883129..2884166 (+) Length   345 a.a.
NCBI ID   WP_308155008.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 24(2023)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2878129..2889166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9R17_RS12895 (Q9R17_12895) soxR 2879137..2879574 (+) 438 WP_308155003.1 redox-sensitive transcriptional activator SoxR -
  Q9R17_RS12900 (Q9R17_12900) - 2879626..2880228 (-) 603 WP_308155004.1 short chain dehydrogenase -
  Q9R17_RS12905 (Q9R17_12905) - 2880323..2881204 (+) 882 WP_308155005.1 LysR family transcriptional regulator -
  Q9R17_RS12910 (Q9R17_12910) proC 2881524..2882345 (-) 822 WP_308155006.1 pyrroline-5-carboxylate reductase -
  Q9R17_RS12915 (Q9R17_12915) - 2882364..2883041 (-) 678 WP_308155007.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9R17_RS12920 (Q9R17_12920) pilT 2883129..2884166 (+) 1038 WP_308155008.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9R17_RS12925 (Q9R17_12925) pilU 2884257..2885399 (+) 1143 WP_308155009.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9R17_RS12930 (Q9R17_12930) - 2885572..2886555 (+) 984 WP_308155010.1 aldo/keto reductase -
  Q9R17_RS12935 (Q9R17_12935) - 2886562..2887458 (-) 897 WP_308155011.1 LysR family transcriptional regulator -
  Q9R17_RS12940 (Q9R17_12940) - 2887586..2888293 (+) 708 WP_308155012.1 YitT family protein -
  Q9R17_RS12945 (Q9R17_12945) - 2888306..2888845 (-) 540 WP_308158341.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6654

>NTDB_id=872157 Q9R17_RS12920 WP_308155008.1 2883129..2884166(+) (pilT) [Stenotrophomonas sp. 24(2023)]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=872157 Q9R17_RS12920 WP_308155008.1 2883129..2884166(+) (pilT) [Stenotrophomonas sp. 24(2023)]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCCTCGGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAACATCCCGGCACTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTGGATTTCTCCTTTGAAATCCCGTCGCTGGCGCGC
TTCCGCGTGAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTCATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCATGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAACTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAAACCGGCCACCTGGTATTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCGTCGACCGCATC
ATCGACGTGTTCCCGGCCGGTGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGCCGTACCGCCGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.362

0.672
  pilT Vibrio cholerae strain A1552

70.517

95.362

0.672
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391