Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9R17_RS12925 Genome accession   NZ_CP133160
Coordinates   2884257..2885399 (+) Length   380 a.a.
NCBI ID   WP_308155009.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 24(2023)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2879257..2890399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9R17_RS12900 (Q9R17_12900) - 2879626..2880228 (-) 603 WP_308155004.1 short chain dehydrogenase -
  Q9R17_RS12905 (Q9R17_12905) - 2880323..2881204 (+) 882 WP_308155005.1 LysR family transcriptional regulator -
  Q9R17_RS12910 (Q9R17_12910) proC 2881524..2882345 (-) 822 WP_308155006.1 pyrroline-5-carboxylate reductase -
  Q9R17_RS12915 (Q9R17_12915) - 2882364..2883041 (-) 678 WP_308155007.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9R17_RS12920 (Q9R17_12920) pilT 2883129..2884166 (+) 1038 WP_308155008.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9R17_RS12925 (Q9R17_12925) pilU 2884257..2885399 (+) 1143 WP_308155009.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9R17_RS12930 (Q9R17_12930) - 2885572..2886555 (+) 984 WP_308155010.1 aldo/keto reductase -
  Q9R17_RS12935 (Q9R17_12935) - 2886562..2887458 (-) 897 WP_308155011.1 LysR family transcriptional regulator -
  Q9R17_RS12940 (Q9R17_12940) - 2887586..2888293 (+) 708 WP_308155012.1 YitT family protein -
  Q9R17_RS12945 (Q9R17_12945) - 2888306..2888845 (-) 540 WP_308158341.1 DNA-3-methyladenine glycosylase I -
  Q9R17_RS12950 (Q9R17_12950) - 2888924..2889490 (+) 567 WP_308155013.1 YqgE/AlgH family protein -
  Q9R17_RS12955 (Q9R17_12955) ruvX 2889483..2889950 (+) 468 WP_308155014.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42369.71 Da        Isoelectric Point: 6.7683

>NTDB_id=872158 Q9R17_RS12925 WP_308155009.1 2884257..2885399(+) (pilU) [Stenotrophomonas sp. 24(2023)]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEIMLGTPLVQDYIREGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISDIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=872158 Q9R17_RS12925 WP_308155009.1 2884257..2885399(+) (pilU) [Stenotrophomonas sp. 24(2023)]
GTGAACACCACCGCCACCACCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACCGCGGGCATGCCGCCGGCCATCAAGGTCAACGGCAAGATCTCGCCCATCACGCAGACGCCGCTCACGCCGCAGCAGA
GCCGCGATCTGGTGTTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAAAAAACCCACGAGTGTAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGTTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGTATCGA
GACGCGCATTCCCACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTGGGCGCGACCGGTACCGGCAAGTCCACCTCGTTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTGGGCAT
CGATACCGACAGCTGGGAAGCAGCACTGAAGAATACCCTGCGCCAGGCACCGGACGTGATCATGATCGGCGAGGTGCGTA
CCCGCGAAGGCATGGACCATGCCATCGCCTTTGCCGAGACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCC
AACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAACCT
CAAGGGCGTGGTCGCCCAGCAGCTGGTGCCCTCGCCCGATGGGCGTTCGCGCAAGGTGGCGATGGAGATCATGCTGGGTA
CGCCGCTGGTGCAGGACTACATCCGCGAAGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGATTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGATGCCCGCACCCTGTCGCAGGGCCTGGATGGCG
TGGAGATTTCCGATATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.112

98.421

0.661
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.09

93.684

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.817

93.421

0.4
  pilT Acinetobacter baumannii strain A118

40.698

90.526

0.368
  pilT Acinetobacter nosocomialis M2

40.698

90.526

0.368
  pilT Acinetobacter baumannii D1279779

40.698

90.526

0.368
  pilT Pseudomonas aeruginosa PAK

40.896

88.158

0.361
  pilT Legionella pneumophila strain Lp02

40.896

88.158

0.361
  pilT Legionella pneumophila strain ERS1305867

40.896

88.158

0.361