Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RBH92_RS01125 Genome accession   NZ_CP133083
Coordinates   216977..218020 (-) Length   347 a.a.
NCBI ID   WP_307932902.1    Uniprot ID   -
Organism   Nitrosomonas sp. sh817     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 211977..223020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH92_RS01075 (RBH92_01080) - 212100..212360 (+) 261 WP_307932893.1 type II toxin-antitoxin system HicA family toxin -
  RBH92_RS01080 (RBH92_01085) - 212353..212562 (+) 210 WP_307932894.1 type II toxin-antitoxin system HicB family antitoxin -
  RBH92_RS01085 (RBH92_01090) - 212559..212732 (+) 174 WP_307932895.1 hypothetical protein -
  RBH92_RS01090 (RBH92_01095) - 212922..213941 (+) 1020 WP_307932896.1 ParA family protein -
  RBH92_RS01095 (RBH92_01100) - 213922..214158 (-) 237 WP_307932897.1 type II toxin-antitoxin system HicB family antitoxin -
  RBH92_RS01100 (RBH92_01105) - 214272..214562 (+) 291 WP_307933905.1 nucleotidyltransferase family protein -
  RBH92_RS01105 (RBH92_01110) - 214643..215098 (+) 456 WP_307932898.1 hypothetical protein -
  RBH92_RS01110 (RBH92_01115) - 215212..215424 (-) 213 WP_307932899.1 type II toxin-antitoxin system HicB family antitoxin -
  RBH92_RS01115 (RBH92_01120) - 215428..215616 (-) 189 WP_307932900.1 type II toxin-antitoxin system HicA family toxin -
  RBH92_RS01120 (RBH92_01125) pilU 215802..216938 (-) 1137 WP_307932901.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RBH92_RS01125 (RBH92_01130) pilT 216977..218020 (-) 1044 WP_307932902.1 type IV pilus twitching motility protein PilT Machinery gene
  RBH92_RS01130 (RBH92_01135) - 218159..219409 (-) 1251 WP_307932903.1 EAL and HDOD domain-containing protein -
  RBH92_RS01135 (RBH92_01140) - 219449..220291 (-) 843 WP_307932904.1 HDOD domain-containing protein -
  RBH92_RS01140 (RBH92_01145) - 220522..220794 (-) 273 WP_292925599.1 YfhL family 4Fe-4S dicluster ferredoxin -
  RBH92_RS01145 (RBH92_01150) coaD 220803..221288 (-) 486 WP_307932905.1 pantetheine-phosphate adenylyltransferase -
  RBH92_RS01150 (RBH92_01155) rsmD 221281..221835 (-) 555 WP_307932906.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38553.36 Da        Isoelectric Point: 7.0293

>NTDB_id=871687 RBH92_RS01125 WP_307932902.1 216977..218020(-) (pilT) [Nitrosomonas sp. sh817]
MNIVELLAFVVKNNASDLHLSAGMPPMIRVHGDIRRINLPEMDHKEVHAMVYDIMNDSQRKFYEENLECDFSFAIPNLAR
FRVNAFNTQRGAAAVMRTIPSKVLSLEDLRAPKIFAEIAKQPRGVVLVTGPTGSGKSTTLAAMINDINENEYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKEMIRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQNAGMQTLDQN
LNDLVKRNLISVADARAQAVNKDNFRI

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=871687 RBH92_RS01125 WP_307932902.1 216977..218020(-) (pilT) [Nitrosomonas sp. sh817]
ATGAATATTGTAGAGTTGCTCGCATTCGTAGTTAAAAACAACGCTTCGGATCTTCACCTATCCGCCGGCATGCCGCCGAT
GATCCGCGTGCACGGCGATATCCGCCGCATCAACTTACCGGAAATGGATCACAAGGAAGTGCATGCGATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAATTTTACGAAGAAAACCTGGAATGCGACTTCTCCTTTGCCATCCCCAATCTGGCGCGT
TTCCGTGTCAATGCTTTCAATACCCAGCGTGGCGCGGCAGCGGTGATGCGGACGATTCCTTCGAAAGTTCTTAGCCTGGA
AGACTTAAGAGCACCGAAAATATTCGCCGAGATCGCCAAACAACCACGCGGCGTGGTCTTGGTCACCGGCCCGACCGGTT
CGGGTAAATCGACCACGCTGGCGGCGATGATCAACGACATCAACGAAAATGAATACGGCCATATCCTGACGCTGGAAGAT
CCGATCGAATTCGTGCACGAAAGCAAAAAGTGCCTGATCAACCAACGCGAAGTCGGCCGGGATACGCACAGTTTCTCCAA
TGCTCTGCGCTCCGCGCTGCGGGAAGACCCGGACATCATTCTGGTCGGTGAGATGCGCGATCTGGAAACCATCCGTTTGG
CGATGACGGCGGCTGAAACCGGTCACTTGGTTTTCGGCACATTGCATACCAGCTCGGCCGCTAAAACGATTGATCGTGTC
ATCGACGTATTCCCGGCGGAAGAAAAAGAAATGATCCGGGCGATGCTGTCGGAATCGCTACGTGCGGTTATTTCGCAGGC
TCTGCTCAAAACCAAGGATGGCAAAGGCCGGGTTGCGGCGCACGAAATCATGATCGGTACCCCGGCAATCCGCAATCTGA
TCCGTGAAGGTAAAGTCGCACAAATGTATTCCGCGATTCAAACCGGGCAGAACGCCGGGATGCAAACGCTGGATCAGAAT
TTGAATGATCTGGTTAAACGCAATCTCATTTCCGTCGCCGACGCCAGAGCGCAAGCCGTCAACAAAGACAATTTCAGAAT
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Neisseria gonorrhoeae MS11

70.725

99.424

0.703
  pilT Neisseria meningitidis 8013

70.725

99.424

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.406

0.648
  pilT Vibrio cholerae strain A1552

66.568

97.406

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Acinetobacter baylyi ADP1

42.286

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.499

100

0.415
  pilU Vibrio cholerae strain A1552

40.346

100

0.403