Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RBH92_RS01120 Genome accession   NZ_CP133083
Coordinates   215802..216938 (-) Length   378 a.a.
NCBI ID   WP_307932901.1    Uniprot ID   -
Organism   Nitrosomonas sp. sh817     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210802..221938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH92_RS01075 (RBH92_01080) - 212100..212360 (+) 261 WP_307932893.1 type II toxin-antitoxin system HicA family toxin -
  RBH92_RS01080 (RBH92_01085) - 212353..212562 (+) 210 WP_307932894.1 type II toxin-antitoxin system HicB family antitoxin -
  RBH92_RS01085 (RBH92_01090) - 212559..212732 (+) 174 WP_307932895.1 hypothetical protein -
  RBH92_RS01090 (RBH92_01095) - 212922..213941 (+) 1020 WP_307932896.1 ParA family protein -
  RBH92_RS01095 (RBH92_01100) - 213922..214158 (-) 237 WP_307932897.1 type II toxin-antitoxin system HicB family antitoxin -
  RBH92_RS01100 (RBH92_01105) - 214272..214562 (+) 291 WP_307933905.1 nucleotidyltransferase family protein -
  RBH92_RS01105 (RBH92_01110) - 214643..215098 (+) 456 WP_307932898.1 hypothetical protein -
  RBH92_RS01110 (RBH92_01115) - 215212..215424 (-) 213 WP_307932899.1 type II toxin-antitoxin system HicB family antitoxin -
  RBH92_RS01115 (RBH92_01120) - 215428..215616 (-) 189 WP_307932900.1 type II toxin-antitoxin system HicA family toxin -
  RBH92_RS01120 (RBH92_01125) pilU 215802..216938 (-) 1137 WP_307932901.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RBH92_RS01125 (RBH92_01130) pilT 216977..218020 (-) 1044 WP_307932902.1 type IV pilus twitching motility protein PilT Machinery gene
  RBH92_RS01130 (RBH92_01135) - 218159..219409 (-) 1251 WP_307932903.1 EAL and HDOD domain-containing protein -
  RBH92_RS01135 (RBH92_01140) - 219449..220291 (-) 843 WP_307932904.1 HDOD domain-containing protein -
  RBH92_RS01140 (RBH92_01145) - 220522..220794 (-) 273 WP_292925599.1 YfhL family 4Fe-4S dicluster ferredoxin -
  RBH92_RS01145 (RBH92_01150) coaD 220803..221288 (-) 486 WP_307932905.1 pantetheine-phosphate adenylyltransferase -
  RBH92_RS01150 (RBH92_01155) rsmD 221281..221835 (-) 555 WP_307932906.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42129.68 Da        Isoelectric Point: 6.7259

>NTDB_id=871686 RBH92_RS01120 WP_307932901.1 215802..216938(-) (pilU) [Nitrosomonas sp. sh817]
MDKDQAAKFMSDLLRLMLSKKASDLFITAGFPPAMKIDGKMTPVSQQSLSLQHTEMLTKSIMNDKQAAEFDATKECNFAI
NPTGIGRFRVNAFVQQQRVGMVLRTITTKIPDFDDLGLPQVLKEVVMSKRGLVIFVGGTGSGKSTSMAALIGHRNKNSYG
HIITIEDPVEYVHEHINCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRPQLLMDLSLNLRSIVAQRLIPLKDAKGRAAAIEVMLNSPLIADLIFKGHVHEIKDIMRKSKELG
MQTFDMALFELYEAGKISYEDALRNADSVNELRLKIKLESNEAKSQDLNSGIAHLEIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=871686 RBH92_RS01120 WP_307932901.1 215802..216938(-) (pilU) [Nitrosomonas sp. sh817]
ATGGATAAAGACCAAGCCGCCAAATTCATGTCCGATCTGCTGCGCTTGATGCTCAGTAAAAAAGCCTCAGATCTGTTCAT
CACCGCTGGTTTCCCGCCAGCGATGAAAATCGATGGAAAAATGACCCCGGTATCCCAGCAATCGCTATCGCTGCAGCATA
CCGAAATGCTCACCAAATCGATCATGAACGACAAGCAAGCGGCGGAATTCGATGCCACCAAAGAATGCAACTTTGCGATC
AACCCAACCGGTATCGGGCGTTTCCGTGTCAATGCATTCGTGCAGCAACAGCGTGTCGGCATGGTATTGCGGACGATTAC
CACCAAAATCCCTGACTTCGACGATTTGGGTTTGCCGCAAGTCTTGAAAGAAGTTGTGATGAGCAAACGCGGCCTGGTGA
TTTTTGTCGGCGGCACCGGTTCGGGAAAATCGACCTCGATGGCGGCATTGATCGGACATCGCAATAAAAACAGCTACGGC
CATATCATCACCATCGAGGATCCGGTGGAATATGTGCATGAACATATCAACTGCGTCATCACCCAGCGTGAAGTCGGTGT
CGATACCGAATCGTGGGAAGCGGCGCTGAAAAATACCCTGCGGCAAGCACCGGACGTCATCCTGATCGGTGAAATCCGCG
ACCGCGAAACCATGGAACATGCGATTGCCTTCGCCGAAACCGGCCATTTGTGCATGGGCACATTGCACGCCAACAGCGCC
AATCAAGCGCTCGACCGGATCATCAATTTCTTCCCGGAAGAACGCCGCCCGCAATTGCTGATGGATTTGTCACTGAATTT
ACGCTCCATCGTGGCGCAACGTTTAATTCCACTGAAAGACGCCAAAGGACGCGCCGCCGCCATTGAGGTTATGCTCAACT
CGCCGCTGATCGCAGACTTGATCTTCAAAGGCCACGTGCACGAAATCAAGGACATCATGCGCAAATCCAAGGAATTGGGC
ATGCAAACCTTCGACATGGCGCTGTTCGAACTATATGAAGCTGGAAAAATCAGCTACGAAGACGCGCTGCGCAACGCCGA
CTCAGTAAACGAACTGCGTCTGAAAATCAAACTGGAAAGCAACGAAGCCAAATCGCAGGATCTCAATTCTGGCATTGCGC
ATTTGGAAATCACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.966

94.709

0.606
  pilU Acinetobacter baylyi ADP1

58.424

97.354

0.569
  pilU Vibrio cholerae strain A1552

55.493

93.915

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

89.153

0.384
  pilT Pseudomonas aeruginosa PAK

42.433

89.153

0.378
  pilT Legionella pneumophila strain Lp02

41.742

88.095

0.368
  pilT Legionella pneumophila strain ERS1305867

41.742

88.095

0.368