Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RA178_RS06830 Genome accession   NZ_CP132914
Coordinates   1503467..1504504 (+) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella oncorhynchi strain Z-P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1498467..1509504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA178_RS06800 (RA178_06795) rdgB 1499488..1500105 (-) 618 WP_131523655.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  RA178_RS06805 (RA178_06800) - 1500228..1500662 (-) 435 WP_105251872.1 DUF4426 domain-containing protein -
  RA178_RS06810 (RA178_06805) yggU 1500798..1501103 (-) 306 WP_168824592.1 DUF167 family protein YggU -
  RA178_RS06815 (RA178_06810) - 1501103..1501651 (-) 549 WP_306685006.1 YggT family protein -
  RA178_RS06820 (RA178_06815) proC 1501698..1502516 (-) 819 WP_168824572.1 pyrroline-5-carboxylate reductase -
  RA178_RS06825 (RA178_06820) - 1502733..1503431 (-) 699 WP_306685007.1 YggS family pyridoxal phosphate-dependent enzyme -
  RA178_RS06830 (RA178_06825) pilT 1503467..1504504 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  RA178_RS06835 (RA178_06830) pilU 1504514..1505626 (+) 1113 WP_028761282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RA178_RS06840 (RA178_06835) - 1505814..1506353 (+) 540 WP_173243652.1 glutathione peroxidase -
  RA178_RS06845 (RA178_06840) hemH 1506386..1507399 (+) 1014 WP_263148734.1 ferrochelatase -
  RA178_RS06850 (RA178_06845) ruvX 1507487..1507912 (-) 426 WP_028761279.1 Holliday junction resolvase RuvX -
  RA178_RS06855 (RA178_06850) - 1507942..1508505 (-) 564 WP_173243658.1 YqgE/AlgH family protein -
  RA178_RS06860 (RA178_06855) yciH 1508572..1508901 (-) 330 WP_263165733.1 stress response translation initiation inhibitor YciH -
  RA178_RS06865 (RA178_06860) - 1509079..1509396 (-) 318 WP_105253083.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=870699 RA178_RS06830 WP_006082517.1 1503467..1504504(+) (pilT) [Shewanella oncorhynchi strain Z-P2]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=870699 RA178_RS06830 WP_006082517.1 1503467..1504504(+) (pilT) [Shewanella oncorhynchi strain Z-P2]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTTGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAAGGTGTACACAGTTTAGTGTACGACA
TAATGAATGATAAGCAGCGTAAGGACTACGAAGAACATTTAGAAATAGATTTCTCGTTTGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCAGCGGCTGTGTTTCGTACCATTCCAAGTGACATTTTGTCACTTGA
GCAATTAGGTGCGCCGGAAATTTTTAAAAAAATTGCCAGTTTCCCTCGCGGATTGGTGCTAGTGACTGGACCTACGGGGT
CAGGTAAGAGTACCACACTTGCCGCCATGGTGGATTACGTGAATGAAAATCGACATGATCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAAAATAAGCAATGTTTGATTAACCAACGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTACGTGAAGACCCAGACGTGATTTTAGTCGGTGAGATGCGTGACCTTGAGACCATCCGTTTGG
CTATGACCGCCGCTGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACTACTTCTGCCGCGAAAACGATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTGCGCACTATGTTGTCTGAATCGCTGCAAGCCGTTATTTCGCAAAC
CCTGATTAAAAAGGTGGGAGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACGCCCGCTATCCGCAACTTAATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCAATTCAAACAGGTATGGCCCATGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACTCGTGAAGATGCAATGGCGAAAAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388