Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RA178_RS06835 Genome accession   NZ_CP132914
Coordinates   1504514..1505626 (+) Length   370 a.a.
NCBI ID   WP_028761282.1    Uniprot ID   -
Organism   Shewanella oncorhynchi strain Z-P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1499514..1510626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA178_RS06805 (RA178_06800) - 1500228..1500662 (-) 435 WP_105251872.1 DUF4426 domain-containing protein -
  RA178_RS06810 (RA178_06805) yggU 1500798..1501103 (-) 306 WP_168824592.1 DUF167 family protein YggU -
  RA178_RS06815 (RA178_06810) - 1501103..1501651 (-) 549 WP_306685006.1 YggT family protein -
  RA178_RS06820 (RA178_06815) proC 1501698..1502516 (-) 819 WP_168824572.1 pyrroline-5-carboxylate reductase -
  RA178_RS06825 (RA178_06820) - 1502733..1503431 (-) 699 WP_306685007.1 YggS family pyridoxal phosphate-dependent enzyme -
  RA178_RS06830 (RA178_06825) pilT 1503467..1504504 (+) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  RA178_RS06835 (RA178_06830) pilU 1504514..1505626 (+) 1113 WP_028761282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RA178_RS06840 (RA178_06835) - 1505814..1506353 (+) 540 WP_173243652.1 glutathione peroxidase -
  RA178_RS06845 (RA178_06840) hemH 1506386..1507399 (+) 1014 WP_263148734.1 ferrochelatase -
  RA178_RS06850 (RA178_06845) ruvX 1507487..1507912 (-) 426 WP_028761279.1 Holliday junction resolvase RuvX -
  RA178_RS06855 (RA178_06850) - 1507942..1508505 (-) 564 WP_173243658.1 YqgE/AlgH family protein -
  RA178_RS06860 (RA178_06855) yciH 1508572..1508901 (-) 330 WP_263165733.1 stress response translation initiation inhibitor YciH -
  RA178_RS06865 (RA178_06860) - 1509079..1509396 (-) 318 WP_105253083.1 hypothetical protein -
  RA178_RS06870 (RA178_06865) - 1509577..1510137 (+) 561 WP_263255231.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41029.16 Da        Isoelectric Point: 6.3720

>NTDB_id=870700 RA178_RS06835 WP_028761282.1 1504514..1505626(+) (pilU) [Shewanella oncorhynchi strain Z-P2]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=870700 RA178_RS06835 WP_028761282.1 1504514..1505626(+) (pilU) [Shewanella oncorhynchi strain Z-P2]
ATGGATGTTCGTCCGTTTTTAAAAGTGATAGTGGAACGCAAAGCCTCCGACTTGTTCATCACTGCGGGTTTTCCGCCGAG
TGCCAAGATTGACGGTGAATTGCGTCCCTTGGCAGAAAGTGCGTTTACACCAGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAGCAAGAGTTTCATTCGACTCGTGAGTGTAACTTCGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCCTTTTGGCAGCGTGAATCGCCAGGCTGTGTGATGCGCCGTATCGAAACTAAAATCCCTGACGTCGA
TGATTTAAAACTGCCACCCATTCTGAAAGATCTGGTGATGAGCAAACGGGGTCTCATTATTATGGTGGGCGGAACGGGTA
CAGGTAAGTCGACATCCCTAGCTGCCTTGGTGGGATATCGTAATGGTCATGCTCGTGGTCATATTCTTACTATCGAAGAC
CCAGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAATCTTTCGATGC
AGCGCTAAAAAGTTCGCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCTGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAACAGTTATTGTTCGATTTGTCATTGAATTTACGCGGGATTGTGGCGCAGCA
ATTGATCCCTAAAGTCGATGGCACTGGGCGTCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTACTCAAAGAAACCATGGCAAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAGGCC
TTGCTGAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCAAACGATTTACGTTT
GATGATTAAATTGCAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362