Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WHU57_RS17990 Genome accession   NZ_CP148323
Coordinates   3688364..3689344 (-) Length   326 a.a.
NCBI ID   WP_001305314.1    Uniprot ID   A0A0H2Z2A2
Organism   Escherichia coli strain Z1323HEC0477     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3683364..3694344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU57_RS17960 (WHU57_17960) yggI 3684029..3684526 (+) 498 WP_112793873.1 SprT family zinc-dependent metalloprotease -
  WHU57_RS17965 (WHU57_17965) endA 3684621..3685328 (+) 708 WP_001305312.1 deoxyribonuclease I -
  WHU57_RS17970 (WHU57_17970) rsmE 3685408..3686139 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WHU57_RS17975 (WHU57_17975) gshB 3686152..3687102 (+) 951 WP_000593259.1 glutathione synthase -
  WHU57_RS17980 (WHU57_17980) yqgE 3687211..3687774 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  WHU57_RS17985 (WHU57_17985) ruvX 3687774..3688190 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  WHU57_RS17990 (WHU57_17990) pilT 3688364..3689344 (-) 981 WP_001305314.1 type IV pilus twitching motility protein PilT Machinery gene
  WHU57_RS17995 (WHU57_17995) yggS 3689362..3690066 (+) 705 WP_000997793.1 pyridoxal phosphate homeostasis protein -
  WHU57_RS18000 (WHU57_18000) yggT 3690084..3690650 (+) 567 WP_338902050.1 osmotic shock tolerance protein YggT -
  WHU57_RS18005 (WHU57_18005) yggU 3690647..3690937 (+) 291 WP_338902052.1 DUF167 family protein YggU -
  WHU57_RS18010 (WHU57_18010) rdgB 3690945..3691538 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  WHU57_RS18015 (WHU57_18015) hemW 3691531..3692667 (+) 1137 WP_000239951.1 radical SAM family heme chaperone HemW -
  WHU57_RS18020 (WHU57_18020) yggM 3692732..3693739 (-) 1008 WP_033553367.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36043.27 Da        Isoelectric Point: 5.9975

>NTDB_id=870174 WHU57_RS17990 WP_001305314.1 3688364..3689344(-) (pilT) [Escherichia coli strain Z1323HEC0477]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=870174 WHU57_RS17990 WP_001305314.1 3688364..3689344(-) (pilT) [Escherichia coli strain Z1323HEC0477]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z2A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362