Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAM11_RS08885 Genome accession   NZ_CP132383
Coordinates   1949768..1950712 (-) Length   314 a.a.
NCBI ID   WP_306243133.1    Uniprot ID   -
Organism   Gilliamella apis strain K-MP18     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1944768..1955712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM11_RS08855 (RAM11_08855) - 1945032..1945514 (+) 483 WP_306243124.1 thermonuclease family protein -
  RAM11_RS08860 (RAM11_08860) - 1945814..1946023 (-) 210 WP_065579252.1 cold shock domain-containing protein -
  RAM11_RS08865 (RAM11_08865) proC 1946315..1947124 (-) 810 WP_306243126.1 pyrroline-5-carboxylate reductase -
  RAM11_RS08870 (RAM11_08870) - 1947196..1947750 (-) 555 WP_306243128.1 hypothetical protein -
  RAM11_RS08875 (RAM11_08875) - 1947892..1948581 (-) 690 WP_306243130.1 hypothetical protein -
  RAM11_RS08880 (RAM11_08880) - 1948664..1949302 (-) 639 WP_306243131.1 hypothetical protein -
  RAM11_RS08885 (RAM11_08885) pilT 1949768..1950712 (-) 945 WP_306243133.1 type IV pilus twitching motility protein PilT Machinery gene
  RAM11_RS08890 (RAM11_08890) - 1950749..1951450 (+) 702 WP_306243135.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAM11_RS08895 (RAM11_08895) - 1951487..1952044 (+) 558 WP_198220561.1 YggT family protein -
  RAM11_RS08900 (RAM11_08900) - 1952072..1952494 (-) 423 WP_086363791.1 YcgN family cysteine cluster protein -
  RAM11_RS08905 (RAM11_08905) - 1952491..1952739 (-) 249 WP_086363790.1 YcgL domain-containing protein -
  RAM11_RS08910 (RAM11_08910) - 1952762..1953721 (-) 960 WP_086363789.1 glucokinase -
  RAM11_RS08915 (RAM11_08915) corA 1953920..1954861 (-) 942 WP_110447549.1 magnesium/cobalt transporter CorA -
  RAM11_RS08920 (RAM11_08920) ssb 1955078..1955593 (+) 516 WP_110447548.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35101.76 Da        Isoelectric Point: 8.6341

>NTDB_id=868922 RAM11_RS08885 WP_306243133.1 1949768..1950712(-) (pilT) [Gilliamella apis strain K-MP18]
MLNSMLAFSLTQKASDLHLSSGEAPCIRINGKLKFISSDKLSPIKLKIELLNLLDDEQKVTLQRNQQIDFAYYLKNVGRF
RVNIFYQQRGISAAFRIINQQIPTLCEIEAPEILKKLVNVESGIILVTGATGSGKSTTLASLIQYINQVAAKHIITLEDP
IEFIYQNEKSLIQQREISHFGEAIDSLLRQDPDIIILGELRNKITIQAALTAAETGHLVFATLHTNSAIQTINRIIDVFE
YGSRELIRTQLSSSLQAIICQKLISYNDTRKAIFEVLINTPAVSNLISEGKIKQILSLMQTGQKSGMKVMSEEK

Nucleotide


Download         Length: 945 bp        

>NTDB_id=868922 RAM11_RS08885 WP_306243133.1 1949768..1950712(-) (pilT) [Gilliamella apis strain K-MP18]
ATGTTAAATTCAATGTTGGCCTTTAGTTTAACGCAAAAAGCCTCTGATTTGCATCTTTCTAGTGGTGAAGCTCCGTGCAT
TCGCATTAATGGAAAATTGAAATTTATCTCAAGTGATAAATTATCACCAATTAAGCTAAAAATTGAGTTATTAAATCTAC
TTGATGATGAGCAAAAAGTAACTTTACAACGAAATCAACAGATTGATTTTGCTTATTATCTTAAGAATGTGGGAAGATTT
AGGGTAAACATTTTCTATCAACAACGAGGAATTTCAGCTGCATTTAGAATTATTAACCAACAAATCCCAACTTTATGTGA
AATTGAGGCACCAGAAATATTGAAAAAACTGGTAAATGTAGAATCAGGAATTATTTTAGTTACTGGTGCAACTGGTAGTG
GGAAATCAACAACATTAGCGTCTTTAATTCAATATATTAATCAAGTTGCAGCTAAACATATTATTACATTAGAAGATCCT
ATTGAGTTTATTTATCAAAATGAAAAATCTTTAATCCAGCAAAGAGAAATCTCACATTTTGGTGAAGCTATTGATAGCTT
ATTAAGACAAGATCCGGATATTATTATATTAGGTGAATTACGTAATAAAATCACTATTCAAGCCGCATTAACTGCTGCCG
AAACTGGGCATTTAGTTTTTGCTACTCTACATACTAATTCAGCAATTCAAACGATTAATCGAATAATTGATGTATTTGAA
TACGGATCACGTGAGTTAATTCGAACACAATTGTCTAGTAGTTTACAAGCAATTATCTGTCAAAAACTAATCTCATATAA
TGATACTCGTAAAGCGATATTTGAAGTACTTATCAATACACCAGCAGTGAGTAATTTAATTAGTGAAGGAAAAATAAAGC
AAATATTGTCGTTAATGCAGACAGGTCAAAAAAGTGGAATGAAAGTGATGAGTGAAGAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

49.211

100

0.497
  pilT Neisseria meningitidis 8013

49.211

100

0.497
  pilT Vibrio cholerae strain A1552

48.882

99.682

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.882

99.682

0.487
  pilT Legionella pneumophila strain ERS1305867

47.152

100

0.475
  pilT Legionella pneumophila strain Lp02

47.152

100

0.475
  pilT Acinetobacter baumannii D1279779

45.886

100

0.462
  pilT Acinetobacter baylyi ADP1

45.886

100

0.462
  pilT Acinetobacter nosocomialis M2

45.886

100

0.462
  pilT Acinetobacter baumannii strain A118

45.886

100

0.462
  pilT Pseudomonas aeruginosa PAK

45.223

100

0.452
  pilT Pseudomonas stutzeri DSM 10701

44.904

100

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.635

100

0.408
  pilU Acinetobacter baylyi ADP1

39.937

100

0.404
  pilU Vibrio cholerae strain A1552

37.224

100

0.376
  pilU Pseudomonas stutzeri DSM 10701

36.943

100

0.369