Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAM03_RS08125 Genome accession   NZ_CP132382
Coordinates   1798037..1798981 (-) Length   314 a.a.
NCBI ID   WP_110453237.1    Uniprot ID   -
Organism   Gilliamella apis strain ESL0169     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1793037..1803981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM03_RS08100 (RAM03_08100) - 1793166..1793648 (+) 483 WP_110453240.1 thermonuclease family protein -
  RAM03_RS08105 (RAM03_08105) cspE 1794076..1794285 (-) 210 WP_065579252.1 transcription antiterminator/RNA stability regulator CspE -
  RAM03_RS08110 (RAM03_08110) proC 1794580..1795389 (-) 810 WP_065579253.1 pyrroline-5-carboxylate reductase -
  RAM03_RS08115 (RAM03_08115) - 1795461..1796015 (-) 555 WP_146207911.1 hypothetical protein -
  RAM03_RS08120 (RAM03_08120) - 1796157..1797899 (-) 1743 WP_306241331.1 PT domain-containing protein -
  RAM03_RS08125 (RAM03_08125) pilT 1798037..1798981 (-) 945 WP_110453237.1 type IV pilus twitching motility protein PilT Machinery gene
  RAM03_RS08130 (RAM03_08130) - 1799018..1799719 (+) 702 WP_110453236.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAM03_RS08135 (RAM03_08135) - 1799756..1800313 (+) 558 WP_086320070.1 YggT family protein -
  RAM03_RS08140 (RAM03_08140) - 1800341..1800763 (-) 423 WP_086320071.1 YcgN family cysteine cluster protein -
  RAM03_RS08145 (RAM03_08145) - 1800760..1801008 (-) 249 WP_081298923.1 YcgL domain-containing protein -
  RAM03_RS08150 (RAM03_08150) - 1801031..1801990 (-) 960 WP_110453235.1 glucokinase -
  RAM03_RS08155 (RAM03_08155) corA 1802190..1803131 (-) 942 WP_065651845.1 magnesium/cobalt transporter CorA -
  RAM03_RS08160 (RAM03_08160) ssb 1803347..1803862 (+) 516 WP_065579262.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35104.73 Da        Isoelectric Point: 7.3996

>NTDB_id=868904 RAM03_RS08125 WP_110453237.1 1798037..1798981(-) (pilT) [Gilliamella apis strain ESL0169]
MLNSMLAFSVTQKASDLHLSSGEVPCIRINGKLKFISSDKLSPVKLKIDLLNLLDDDQKVTLQQHQQIDFAYYLENVGRF
RVNIFYQQRGISAVFRIINQQIPTLCEIEAPEILKKLVNVESGIILVTGATGSGKSTTLAALIQYINQIAAKHIITLEDP
IEFIYQNEKSLIQQREISHFGEAIDSLLRQDPDIIMFGELRNKTTIQTALIAAETGHLVFATLHTNSAIETINRIIDVFE
YGSRELIRTQLSSSLQAIICQKLIAYNDTRKAIFEVLINTPAVSNLISEGKIKQILSLMQTGQKSGMKLMSGEK

Nucleotide


Download         Length: 945 bp        

>NTDB_id=868904 RAM03_RS08125 WP_110453237.1 1798037..1798981(-) (pilT) [Gilliamella apis strain ESL0169]
ATGTTAAATTCAATGTTGGCCTTTAGTGTAACGCAAAAAGCCTCTGATTTGCATCTTTCTAGTGGTGAAGTACCGTGCAT
TCGCATTAATGGAAAATTGAAATTTATCTCAAGTGATAAATTATCACCAGTTAAGCTAAAAATTGATTTATTAAATTTAC
TCGATGATGATCAAAAAGTAACTTTACAACAACATCAGCAGATTGATTTTGCTTATTATCTTGAGAATGTGGGAAGATTT
AGGGTAAATATTTTCTATCAACAACGTGGAATTTCAGCTGTATTTAGAATTATTAACCAACAAATCCCAACTTTATGTGA
AATTGAGGCACCGGAAATATTGAAAAAACTGGTAAATGTAGAATCAGGAATTATTTTAGTTACTGGTGCAACTGGTAGTG
GGAAATCAACAACATTAGCGGCTTTGATTCAATATATTAATCAGATTGCCGCTAAACATATTATTACATTAGAAGATCCC
ATTGAGTTTATTTATCAAAATGAAAAGTCTCTAATCCAGCAAAGAGAAATCTCACATTTTGGTGAAGCAATTGATAGTTT
ATTAAGGCAAGACCCAGATATTATTATGTTCGGTGAATTACGTAATAAAACCACGATTCAAACCGCATTAATTGCTGCTG
AAACTGGGCATTTAGTTTTTGCTACTCTACATACTAATTCAGCAATTGAAACTATTAATCGTATAATTGATGTATTTGAA
TACGGATCACGTGAGTTAATACGAACTCAATTATCTAGTAGTTTACAAGCAATTATCTGTCAAAAACTAATCGCATATAA
TGATACTCGCAAAGCGATATTCGAAGTGCTTATTAATACACCTGCAGTGAGTAATTTAATTAGTGAAGGTAAAATAAAAC
AAATATTGTCGTTAATGCAGACTGGTCAAAAAAGTGGAATGAAATTGATGAGTGGGGAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

49.841

100

0.5
  pilT Neisseria meningitidis 8013

49.841

100

0.5
  pilT Vibrio cholerae strain A1552

49.518

99.045

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.518

99.045

0.49
  pilT Legionella pneumophila strain Lp02

47.937

100

0.481
  pilT Legionella pneumophila strain ERS1305867

47.937

100

0.481
  pilT Acinetobacter baumannii D1279779

47.267

99.045

0.468
  pilT Acinetobacter baylyi ADP1

47.267

99.045

0.468
  pilT Acinetobacter nosocomialis M2

47.267

99.045

0.468
  pilT Acinetobacter baumannii strain A118

47.267

99.045

0.468
  pilT Pseudomonas aeruginosa PAK

45.541

100

0.455
  pilT Pseudomonas stutzeri DSM 10701

45.223

100

0.452
  pilU Acinetobacter baylyi ADP1

40.446

100

0.404
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.317

100

0.404
  pilU Vibrio cholerae strain A1552

37.7

99.682

0.376
  pilU Pseudomonas stutzeri DSM 10701

37.742

98.726

0.373