Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RAM17_RS09100 Genome accession   NZ_CP132381
Coordinates   1980497..1981441 (-) Length   314 a.a.
NCBI ID   WP_110447553.1    Uniprot ID   A0A2V4DNH5
Organism   Gilliamella apis strain ESL0172     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1975497..1986441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM17_RS09075 (RAM17_09075) - 1975645..1976127 (+) 483 WP_110447557.1 thermonuclease family protein -
  RAM17_RS09080 (RAM17_09080) - 1976545..1976754 (-) 210 WP_065579252.1 cold shock domain-containing protein -
  RAM17_RS09085 (RAM17_09085) proC 1977046..1977855 (-) 810 WP_110447556.1 pyrroline-5-carboxylate reductase -
  RAM17_RS09090 (RAM17_09090) - 1977927..1978481 (-) 555 WP_110447555.1 hypothetical protein -
  RAM17_RS09095 (RAM17_09095) - 1978623..1980032 (-) 1410 WP_110447554.1 hypothetical protein -
  RAM17_RS09100 (RAM17_09100) pilT 1980497..1981441 (-) 945 WP_110447553.1 type IV pilus twitching motility protein PilT Machinery gene
  RAM17_RS09105 (RAM17_09105) - 1981478..1982179 (+) 702 WP_110447552.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAM17_RS09110 (RAM17_09110) - 1982216..1982773 (+) 558 WP_110447551.1 YggT family protein -
  RAM17_RS09115 (RAM17_09115) - 1982801..1983223 (-) 423 WP_086363791.1 YcgN family cysteine cluster protein -
  RAM17_RS09120 (RAM17_09120) - 1983220..1983468 (-) 249 WP_086363790.1 YcgL domain-containing protein -
  RAM17_RS09125 (RAM17_09125) - 1983491..1984450 (-) 960 Protein_1775 glucokinase -
  RAM17_RS09130 (RAM17_09130) corA 1984649..1985590 (-) 942 WP_110447549.1 magnesium/cobalt transporter CorA -
  RAM17_RS09135 (RAM17_09135) ssb 1985807..1986322 (+) 516 WP_110447548.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35119.79 Da        Isoelectric Point: 8.2513

>NTDB_id=868885 RAM17_RS09100 WP_110447553.1 1980497..1981441(-) (pilT) [Gilliamella apis strain ESL0172]
MLNSMLAFSLTQKASDLHLSSGEAPCIRINGKLKFISSDKLSPIKLKIELLNLLDDEQKVTLQQNQQIDFAYYLKNVGRF
RVNIFYQQRGISAAFRIINQQIPTLCEIEAPEILKKLVNVESGIILVTGATGSGKSTTLASLIQYINQVAAKHIITLEDP
IEFIYQNEKSLIQQREISHFGEVIDSLLRQDPDIIMLGELRNKITIQAALTAAETGHLVFATLHTNSAIQTINRIIDVFE
YGSRELIRTQLSSSLQAIICQKLISYNDTRKAIFEVLINTPAVSNLISEGKIKQILSLMQTGQKSGMKVMSEEK

Nucleotide


Download         Length: 945 bp        

>NTDB_id=868885 RAM17_RS09100 WP_110447553.1 1980497..1981441(-) (pilT) [Gilliamella apis strain ESL0172]
ATGTTAAATTCAATGTTGGCCTTTAGTTTAACGCAAAAAGCCTCTGATTTGCATCTTTCTAGTGGTGAAGCTCCGTGCAT
TCGCATTAATGGAAAATTGAAATTTATCTCAAGTGATAAATTATCACCAATTAAGCTAAAAATTGAGTTATTAAATCTAC
TTGATGATGAGCAAAAAGTAACTTTACAACAAAATCAACAGATTGATTTTGCTTATTATCTTAAGAATGTGGGAAGATTT
AGGGTAAATATTTTCTATCAACAACGTGGAATTTCAGCTGCATTTAGAATTATTAACCAACAAATCCCAACTTTATGTGA
AATTGAGGCACCAGAAATATTGAAAAAACTGGTAAATGTAGAATCAGGAATTATTTTAGTTACTGGTGCAACTGGTAGTG
GGAAATCAACAACATTAGCGTCTTTAATTCAATATATTAATCAGGTTGCAGCTAAACATATTATCACATTAGAAGATCCT
ATTGAGTTTATTTATCAAAATGAAAAATCTCTAATCCAGCAAAGAGAAATCTCACATTTTGGTGAAGTTATTGATAGCTT
ATTAAGACAAGATCCGGATATTATTATGTTAGGTGAATTACGTAATAAAATCACTATTCAAGCCGCATTAACTGCTGCCG
AAACTGGGCATTTAGTTTTTGCTACTCTACATACTAATTCAGCAATTCAAACGATTAATCGAATAATTGATGTATTTGAA
TACGGATCACGTGAGTTAATTCGAACACAATTGTCTAGTAGTTTACAAGCAATTATCTGTCAAAAACTAATCTCATATAA
TGATACTCGTAAAGCGATATTTGAAGTACTTATCAATACACCTGCAGTGAGTAATTTAATTAGTGAAGGAAAAATAAAGC
AAATATTGTCATTAATGCAGACTGGTCAAAAAAGTGGAATGAAAGTGATGAGTGAAGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V4DNH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

49.211

100

0.497
  pilT Neisseria meningitidis 8013

49.211

100

0.497
  pilT Vibrio cholerae strain A1552

48.562

99.682

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.562

99.682

0.484
  pilT Legionella pneumophila strain ERS1305867

46.835

100

0.471
  pilT Legionella pneumophila strain Lp02

46.835

100

0.471
  pilT Acinetobacter baumannii strain A118

45.57

100

0.459
  pilT Acinetobacter baylyi ADP1

45.57

100

0.459
  pilT Acinetobacter baumannii D1279779

45.57

100

0.459
  pilT Acinetobacter nosocomialis M2

45.57

100

0.459
  pilT Pseudomonas aeruginosa PAK

44.904

100

0.449
  pilT Pseudomonas stutzeri DSM 10701

44.586

100

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.317

100

0.404
  pilU Acinetobacter baylyi ADP1

39.623

100

0.401
  pilU Vibrio cholerae strain A1552

37.224

100

0.376
  pilU Pseudomonas stutzeri DSM 10701

36.624

100

0.366