Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O5O45_RS28570 Genome accession   NZ_CP132203
Coordinates   6428926..6429960 (+) Length   344 a.a.
NCBI ID   WP_011400052.1    Uniprot ID   A0AAE5XN00
Organism   Hahella sp. HNIBRBA332     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6423926..6434960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O45_RS28545 (O5O45_28545) - 6424090..6426048 (-) 1959 WP_305902691.1 dynamin family protein -
  O5O45_RS28550 (O5O45_28550) - 6426234..6426542 (-) 309 WP_305902692.1 DUF167 family protein -
  O5O45_RS28555 (O5O45_28555) - 6426535..6427074 (-) 540 WP_305902693.1 YggT family protein -
  O5O45_RS28560 (O5O45_28560) proC 6427149..6427979 (-) 831 WP_305902694.1 pyrroline-5-carboxylate reductase -
  O5O45_RS28565 (O5O45_28565) - 6428085..6428768 (-) 684 WP_305902695.1 YggS family pyridoxal phosphate-dependent enzyme -
  O5O45_RS28570 (O5O45_28570) pilT 6428926..6429960 (+) 1035 WP_011400052.1 type IV pilus twitching motility protein PilT Machinery gene
  O5O45_RS28575 (O5O45_28575) pilU 6430023..6431150 (+) 1128 WP_216737532.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O5O45_RS28580 (O5O45_28580) polA 6431234..6433969 (-) 2736 WP_305902696.1 DNA polymerase I -
  O5O45_RS28585 (O5O45_28585) - 6434156..6434434 (+) 279 WP_216737530.1 DUF2782 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38015.82 Da        Isoelectric Point: 6.3477

>NTDB_id=867649 O5O45_RS28570 WP_011400052.1 6428926..6429960(+) (pilT) [Hahella sp. HNIBRBA332]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGQVFKDIALKPRGLCLVTGPTGSGKSTTLAAMIDFINDTRYDHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGAALGMQTLDQCL
HNLISKGLISREAAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=867649 O5O45_RS28570 WP_011400052.1 6428926..6429960(+) (pilT) [Hahella sp. HNIBRBA332]
ATGGACATTACCGAGTTGCTTGCGTTTTCTGCTAAGCAGGGCGCTTCCGACTTACACCTATCTGCCGGGTTGCCTCCCAT
GATACGCGTCGACGGGGATGTCAGGCGAATTAACCTGCCCGCGATGGAGCATAAAGAAGTACACGCGCTGATTTACGATA
TTATGAACGATAAACAGCGTAAGGATTTCGAAGAGTTTCTGGAGACGGACTTCTCGTTCGAAGTTCCCGGTGTCGCCCGT
TTCCGGGTTAACGCCTTTAACCAGAACCGCGGCGCTGGCGCCGTATTCCGGACTATCCCCTCCAAAGTGCTGACAATGGA
AGACTTGGGAATGGGGCAGGTGTTCAAGGATATCGCCCTGAAGCCTCGCGGACTCTGTCTGGTCACCGGACCGACCGGTT
CCGGTAAGAGTACGACGCTGGCGGCGATGATCGACTTTATCAACGATACCCGTTACGACCACATCCTCACTATCGAAGAC
CCTATCGAGTTCGTGCATGAAAGTAAGAAATGTCTGGTGAACCAGCGTGAAGTACACCGTGACACGCTTGGGTTCAGTGA
AGCGCTGCGTTCAGCTTTGCGGGAAGATCCGGACATTATCCTGGTTGGTGAGATGCGGGACTTGGAAACCATCCGTTTGG
CGTTGACCGCTGCGGAAACCGGGCACTTGGTATTCGGCACCCTGCATACCACCTCCGCCGCGAAAACCATTGACCGGGTG
GTCGACGTGTTTCCTGCGGAAGAAAAAGCCATGGTAAGATCAATGCTCTCCGAATCGTTACAGGCGGTCGTCTCGCAGAC
TCTTCTGAAGAAGGTCGGCGGCGGTCGTGTGGCGGCCCATGAAATCATGATCGGCACGCCAGCGATTCGTAACCTGATAC
GCGAAGACAAAGTCGCCCAGATGTATTCGTCTATCCAGACCGGCGCCGCGTTGGGTATGCAGACACTTGACCAGTGTCTG
CACAATTTGATTTCTAAAGGGCTGATCAGCCGCGAAGCCGCGCGCGAAAAAGCCAAAATGCCGGAGAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

91.57

100

0.916
  pilT Pseudomonas aeruginosa PAK

90.988

100

0.91
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.837

0.672
  pilT Vibrio cholerae strain A1552

67.941

98.837

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.384

0.416
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398