Detailed information NTG0867650

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O5O45_RS28575
• Genome accession: NZ_CP132203
• Coordinates: 6430023..6431150 (+)
• Length: 375 a.a.
• NCBI ID: WP_216737532.1
• Uniprot ID: -
• Organism: Hahella sp. HNIBRBA332
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6425023..6436150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O5O45_RS28550 (O5O45_28550)	-	6426234..6426542 (-)	309	WP_305902692.1	DUF167 family protein	-
O5O45_RS28555 (O5O45_28555)	-	6426535..6427074 (-)	540	WP_305902693.1	YggT family protein	-
O5O45_RS28560 (O5O45_28560)	proC	6427149..6427979 (-)	831	WP_305902694.1	pyrroline-5-carboxylate reductase	-
O5O45_RS28565 (O5O45_28565)	-	6428085..6428768 (-)	684	WP_305902695.1	YggS family pyridoxal phosphate-dependent enzyme	-
O5O45_RS28570 (O5O45_28570)	pilT	6428926..6429960 (+)	1035	WP_011400052.1	type IV pilus twitching motility protein PilT	Machinery gene
O5O45_RS28575 (O5O45_28575)	pilU	6430023..6431150 (+)	1128	WP_216737532.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O5O45_RS28580 (O5O45_28580)	polA	6431234..6433969 (-)	2736	WP_305902696.1	DNA polymerase I	-
O5O45_RS28585 (O5O45_28585)	-	6434156..6434434 (+)	279	WP_216737530.1	DUF2782 domain-containing protein	-
O5O45_RS28590 (O5O45_28590)	-	6434519..6435484 (+)	966	WP_305902697.1	homoserine kinase	-
O5O45_RS28595 (O5O45_28595)	-	6435527..6436012 (-)	486	WP_305902698.1	fibronectin type III domain-containing protein	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 41478.64 Da
• Isoelectric Point: 6.8225

>NTDB_id=867650 O5O45_RS28575 WP_216737532.1 6430023..6431150(+) (pilU) [Hahella sp. HNIBRBA332]
MDFDKLLKIMVEKGASDLFITAGVPPSIKVHGRMVPITKSPLSPEQTRETVFSIMSEKQRADFEVAHECNFAISARGIGR
FRVSAFYQRNLCGMVLRRIETNIPKLDDLHLPEVIKSLSMTKRGLIIFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEYIHQHHGCIITQREVGLDTESFEVALKNTLRQAPDVIMIGEVRSADTMEYAVTFAETGHLCLATLHANNANQALDRI
INFFPPEQHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAIVEILINSPLAADLIRKGEVHKLKELMTKSSELGMQTFDQA
LYRAYAAGEITYEDALSHADSANDLRLMIKLGADSNISKLQGAASSLSLSDDQDY

Nucleotide

• Length: 1128 bp

>NTDB_id=867650 O5O45_RS28575 WP_216737532.1 6430023..6431150(+) (pilU) [Hahella sp. HNIBRBA332]
ATGGATTTCGACAAGCTGTTAAAAATTATGGTGGAAAAGGGGGCTTCGGACCTCTTTATCACAGCTGGGGTGCCGCCCAG
CATCAAAGTTCACGGCCGCATGGTGCCTATCACCAAGTCGCCGCTGTCCCCTGAACAGACACGGGAAACCGTGTTTTCCA
TAATGTCGGAAAAGCAGCGTGCGGATTTTGAAGTCGCGCATGAGTGCAACTTTGCAATCAGCGCCCGCGGCATTGGCCGT
TTCCGGGTCAGCGCCTTTTATCAGCGCAACCTGTGCGGGATGGTGTTGCGTCGAATTGAGACCAACATTCCGAAACTGGA
CGACCTGCATCTGCCGGAGGTTATCAAGTCTCTGTCTATGACCAAGCGGGGCCTGATTATCTTCGTCGGCGCCACCGGCA
CCGGTAAGTCCACGTCGTTGGCGGCGATGATCGGCCACCGGAATCGCAACAGCAAAGGCCACATCATTTCCATCGAGGAC
CCTATCGAATATATCCATCAGCACCACGGCTGCATTATTACGCAGCGGGAAGTGGGACTGGATACGGAGTCGTTCGAAGT
GGCTCTGAAGAACACGCTGCGGCAGGCTCCCGACGTTATCATGATCGGGGAGGTGCGCTCTGCCGATACCATGGAATATG
CGGTGACCTTCGCGGAAACCGGTCATTTGTGTTTGGCGACATTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGCCTGAGCAGCATAACCAGGTGTGGATGGACTTGTCCTTGAACTTGAAGGCCATCGTGGCGCAGCA
ATTGGTGCCGACTCCAGACGGTAAGGGGCGCCGGGCGATTGTTGAGATTCTCATCAACTCGCCGCTGGCCGCAGACTTGA
TTCGTAAAGGGGAAGTGCACAAGTTGAAAGAGCTGATGACCAAGTCGTCGGAACTGGGCATGCAGACTTTCGATCAGGCG
CTATACCGCGCTTACGCTGCGGGAGAGATTACCTACGAAGACGCGTTGTCACACGCGGACTCCGCAAACGATCTGCGTCT
GATGATCAAACTGGGCGCGGACAGCAATATTTCCAAGCTGCAAGGCGCCGCCAGCAGTCTGTCTTTGAGCGACGATCAGG
ATTATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	76.203	99.733	0.76
pilU	Acinetobacter baylyi ADP1	64.426	95.2	0.613
pilU	Vibrio cholerae strain A1552	55.923	96.8	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	90.4	0.387
pilT	Pseudomonas aeruginosa PAK	40.708	90.4	0.368
pilT	Acinetobacter baumannii D1279779	40.118	90.4	0.363
pilT	Acinetobacter baumannii strain A118	40.118	90.4	0.363