Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q9L40_RS09775 Genome accession   NZ_CP132190
Coordinates   1013665..1014702 (-) Length   345 a.a.
NCBI ID   WP_000350202.1    Uniprot ID   -
Organism   Vibrio cholerae strain DRC187     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1008665..1019702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L40_RS09750 (Q9L40_09750) - 1009149..1009712 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  Q9L40_RS09755 (Q9L40_09755) ruvX 1009793..1010215 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  Q9L40_RS09760 (Q9L40_09760) tyrS 1010240..1011520 (-) 1281 WP_305799176.1 tyrosine--tRNA ligase -
  Q9L40_RS09765 (Q9L40_09765) - 1011629..1012426 (-) 798 WP_305799175.1 helix-turn-helix transcriptional regulator -
  Q9L40_RS09770 (Q9L40_09770) pilU 1012544..1013650 (-) 1107 WP_057644481.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9L40_RS09775 (Q9L40_09775) pilT 1013665..1014702 (-) 1038 WP_000350202.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9L40_RS09780 (Q9L40_09780) - 1014728..1015438 (+) 711 WP_001256658.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9L40_RS09785 (Q9L40_09785) proC 1015491..1016309 (+) 819 WP_113601002.1 pyrroline-5-carboxylate reductase -
  Q9L40_RS09790 (Q9L40_09790) - 1016363..1016920 (+) 558 WP_305799174.1 YggT family protein -
  Q9L40_RS09795 (Q9L40_09795) yggU 1016920..1017210 (+) 291 WP_305799173.1 DUF167 family protein YggU -
  Q9L40_RS09800 (Q9L40_09800) - 1017256..1017687 (+) 432 WP_305799172.1 DUF4426 domain-containing protein -
  Q9L40_RS09805 (Q9L40_09805) - 1017757..1018359 (+) 603 WP_113611152.1 XTP/dITP diphosphatase -
  Q9L40_RS09810 (Q9L40_09810) hemW 1018353..1019528 (+) 1176 WP_305799171.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38190.78 Da        Isoelectric Point: 6.6672

>NTDB_id=867506 Q9L40_RS09775 WP_000350202.1 1013665..1014702(-) (pilT) [Vibrio cholerae strain DRC187]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDGQRSEYEEKLEVDFSFELPSVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=867506 Q9L40_RS09775 WP_000350202.1 1013665..1014702(-) (pilT) [Vibrio cholerae strain DRC187]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACACCGTTTGATTTTTGAGA
TTATGAACGATGGTCAACGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAGTGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTTATCCCGACGTTAGA
GCAATTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CAGGAAAGTCCACAACTTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAGCGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGTTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
GCTCCTAAAACGCGTCGGGGGGGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACTATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.42

100

0.994
  pilT Vibrio cholerae strain A1552

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

71.429

97.391

0.696
  pilT Acinetobacter baumannii strain A118

71.429

97.391

0.696
  pilT Acinetobacter nosocomialis M2

71.131

97.391

0.693
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377