Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9L40_RS09770 Genome accession   NZ_CP132190
Coordinates   1012544..1013650 (-) Length   368 a.a.
NCBI ID   WP_057644481.1    Uniprot ID   -
Organism   Vibrio cholerae strain DRC187     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1007544..1018650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L40_RS09745 (Q9L40_09745) gshB 1008157..1009113 (+) 957 WP_000593310.1 glutathione synthase -
  Q9L40_RS09750 (Q9L40_09750) - 1009149..1009712 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  Q9L40_RS09755 (Q9L40_09755) ruvX 1009793..1010215 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  Q9L40_RS09760 (Q9L40_09760) tyrS 1010240..1011520 (-) 1281 WP_305799176.1 tyrosine--tRNA ligase -
  Q9L40_RS09765 (Q9L40_09765) - 1011629..1012426 (-) 798 WP_305799175.1 helix-turn-helix transcriptional regulator -
  Q9L40_RS09770 (Q9L40_09770) pilU 1012544..1013650 (-) 1107 WP_057644481.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9L40_RS09775 (Q9L40_09775) pilT 1013665..1014702 (-) 1038 WP_000350202.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9L40_RS09780 (Q9L40_09780) - 1014728..1015438 (+) 711 WP_001256658.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9L40_RS09785 (Q9L40_09785) proC 1015491..1016309 (+) 819 WP_113601002.1 pyrroline-5-carboxylate reductase -
  Q9L40_RS09790 (Q9L40_09790) - 1016363..1016920 (+) 558 WP_305799174.1 YggT family protein -
  Q9L40_RS09795 (Q9L40_09795) yggU 1016920..1017210 (+) 291 WP_305799173.1 DUF167 family protein YggU -
  Q9L40_RS09800 (Q9L40_09800) - 1017256..1017687 (+) 432 WP_305799172.1 DUF4426 domain-containing protein -
  Q9L40_RS09805 (Q9L40_09805) - 1017757..1018359 (+) 603 WP_113611152.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41365.35 Da        Isoelectric Point: 6.5076

>NTDB_id=867505 Q9L40_RS09770 WP_057644481.1 1012544..1013650(-) (pilU) [Vibrio cholerae strain DRC187]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEVLSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEILLNSPRISDLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=867505 Q9L40_RS09770 WP_057644481.1 1012544..1013650(-) (pilU) [Vibrio cholerae strain DRC187]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCGCCGATTTT
ATATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGTGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTACCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGTGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACGGGGTACCGAAACCAGCACCGTACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTACATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTTGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGCGAAATCCGTAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACTTTGCACGCCAATAACGCCAACCAAGCCTTAGAGCGGATTTTGCAT
CTGGTGCCCAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAATTCTGCTCAATAGCCCGCGCATTTCCGATTTGATCCGCC
GTGGCGAGCTACACGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACCTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCTGACTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.185

100

0.992
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

43.068

92.12

0.397
  pilT Legionella pneumophila strain ERS1305867

43.068

92.12

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.617

94.293

0.364