Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5621_RS14285 Genome accession   NZ_AP024618
Coordinates   3152105..3153142 (-) Length   345 a.a.
NCBI ID   WP_025009461.1    Uniprot ID   A0A1S2TIJ8
Organism   Shewanella carassii strain TUM17387     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3147105..3158142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5621_RS14260 (TUM17387_27830) - 3147109..3147843 (-) 735 WP_071237900.1 tryptophan 2,3-dioxygenase family protein -
  K5621_RS14265 (TUM17387_27840) hemH 3148015..3149028 (-) 1014 WP_044733989.1 ferrochelatase -
  K5621_RS14270 (TUM17387_27850) - 3149063..3149659 (-) 597 WP_221049562.1 glutathione peroxidase -
  K5621_RS14275 (TUM17387_27860) - 3149717..3150826 (-) 1110 WP_221049563.1 hypothetical protein -
  K5621_RS14280 (TUM17387_27870) pilU 3150983..3152095 (-) 1113 WP_039034904.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5621_RS14285 (TUM17387_27880) pilT 3152105..3153142 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  K5621_RS14290 (TUM17387_27890) - 3153179..3153874 (+) 696 WP_221049564.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5621_RS14295 (TUM17387_27900) proC 3154007..3154825 (+) 819 WP_221049565.1 pyrroline-5-carboxylate reductase -
  K5621_RS14300 (TUM17387_27910) - 3154849..3155400 (+) 552 WP_039034907.1 YggT family protein -
  K5621_RS14305 (TUM17387_27920) yggU 3155400..3155687 (+) 288 WP_219160885.1 DUF167 family protein YggU -
  K5621_RS14310 (TUM17387_27930) - 3155750..3156184 (+) 435 WP_221049566.1 DUF4426 domain-containing protein -
  K5621_RS14315 (TUM17387_27940) rdgB 3156444..3157046 (+) 603 WP_221049567.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.97 Da        Isoelectric Point: 6.8739

>NTDB_id=86685 K5621_RS14285 WP_025009461.1 3152105..3153142(-) (pilT) [Shewanella carassii strain TUM17387]
MEITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINVPALDHQGVHRLVYDIMNDKQRKDYEEFLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILTLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QNLVNRGLISREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=86685 K5621_RS14285 WP_025009461.1 3152105..3153142(-) (pilT) [Shewanella carassii strain TUM17387]
ATGGAAATCACTGAATTATTGGCCTTTAGTGTAAAGCATAACGCTTCGGATCTGCACCTGTCGGCCGGTGTGCCTCCCAT
GATACGCGTCGATGGCGAGGTCAGAAAGATTAATGTCCCTGCGCTTGATCACCAAGGGGTTCACAGACTTGTCTACGACA
TAATGAATGACAAGCAGCGTAAGGATTATGAAGAGTTTCTGGAAATCGACTTTTCCTTTGAAGTTCCCAATCTGGCGCGT
TTTCGTGTCAATGCCTTCAATCAGTCCCGCGGCGCCGCCGCGGTTTTTCGTACTATTCCCAGCGACATTTTGACTTTGGA
ACAACTGGGCGCGCCGGAAATCTTCAAGAAAATCGCTTCATATCCTCGCGGCCTGGTGCTGGTAACCGGCCCGACAGGTT
CGGGTAAGAGTACCACCCTGGCGGCGATGATAGATTACGTCAATGAGAACCGCCACGATCATATTCTCACCATTGAAGAC
CCTATCGAATTCGTGCACCAAAACAAGCAATGCCTTATCAACCAGCGGGAAGTGCACCGCCATACCCACAGCTTTAACGC
CGCCCTCAGAAGCGCCTTGCGTGAAGACCCGGATGTCATCCTGGTCGGTGAGATGCGGGATTTGGAAACCATACGCCTGG
CGATGACCGCAGCAGAAACCGGCCACTTGGTGTTCGGTACCTTGCACACCACCTCGGCGGCCAAGACCATTGACCGTGTG
GTTGACGTATTCCCCGCAGGCGAGAAGGACATGGTGCGTACCATGTTGTCTGAGTCGTTGCAGGCGGTTATCTCCCAGAC
CCTGATCAAGAAGGTTGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGATGGGTACCCCGGCTATCCGAAACCTTATCC
GTGAGGACAAGGTGGCACAGATGTATTCTGCTATCCAAACCGGTATGGCTCATGGCATGCAAACCTTGGAGCAGAGCCTG
CAGAACCTGGTGAATCGCGGCTTGATCAGTCGGGAAGATGCCATGGCCAAGAGTTCCAACAAACAAGCGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2TIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.871

99.13

0.812
  pilT Acinetobacter baumannii D1279779

81.871

99.13

0.812
  pilT Acinetobacter baumannii strain A118

81.871

99.13

0.812
  pilT Acinetobacter baylyi ADP1

78.426

99.42

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment