Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5621_RS14280 Genome accession   NZ_AP024618
Coordinates   3150983..3152095 (-) Length   370 a.a.
NCBI ID   WP_039034904.1    Uniprot ID   -
Organism   Shewanella carassii strain TUM17387     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3145983..3157095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5621_RS14260 (TUM17387_27830) - 3147109..3147843 (-) 735 WP_071237900.1 tryptophan 2,3-dioxygenase family protein -
  K5621_RS14265 (TUM17387_27840) hemH 3148015..3149028 (-) 1014 WP_044733989.1 ferrochelatase -
  K5621_RS14270 (TUM17387_27850) - 3149063..3149659 (-) 597 WP_221049562.1 glutathione peroxidase -
  K5621_RS14275 (TUM17387_27860) - 3149717..3150826 (-) 1110 WP_221049563.1 hypothetical protein -
  K5621_RS14280 (TUM17387_27870) pilU 3150983..3152095 (-) 1113 WP_039034904.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5621_RS14285 (TUM17387_27880) pilT 3152105..3153142 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  K5621_RS14290 (TUM17387_27890) - 3153179..3153874 (+) 696 WP_221049564.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5621_RS14295 (TUM17387_27900) proC 3154007..3154825 (+) 819 WP_221049565.1 pyrroline-5-carboxylate reductase -
  K5621_RS14300 (TUM17387_27910) - 3154849..3155400 (+) 552 WP_039034907.1 YggT family protein -
  K5621_RS14305 (TUM17387_27920) yggU 3155400..3155687 (+) 288 WP_219160885.1 DUF167 family protein YggU -
  K5621_RS14310 (TUM17387_27930) - 3155750..3156184 (+) 435 WP_221049566.1 DUF4426 domain-containing protein -
  K5621_RS14315 (TUM17387_27940) rdgB 3156444..3157046 (+) 603 WP_221049567.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41055.15 Da        Isoelectric Point: 6.3711

>NTDB_id=86684 K5621_RS14280 WP_039034904.1 3150983..3152095(-) (pilU) [Shewanella carassii strain TUM17387]
MDVRPFLKAMVERKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKETMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=86684 K5621_RS14280 WP_039034904.1 3150983..3152095(-) (pilU) [Shewanella carassii strain TUM17387]
ATGGACGTCAGACCTTTTCTCAAGGCTATGGTGGAGCGTAAGGCCTCGGATCTCTTTATTACCGCGGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGACCCTTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACGCCGGAGCAGAAAAAAGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAGGAGTTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGCATCGAAACCAAGATCCCTACGGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGACTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTAGGGGGAACCGGTA
CGGGTAAGTCTACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCCAATGCCAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACTCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCTGAGAGTAAGCACCAACAGCTCTTGTTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GCTGGTACCCAAGGTGGACGGCAGCGGTCGGCGCGCCGCCATCGAAGTGCTGATTAACACTCCCAGGGTGGCTAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTTAAGGAGACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGATCAGGCG
CTGTTCAAGCTCTACTGTGAAGGGGAGATCAGTTACGCCGATGCACTGCATCATGCTGATTCGCCAAACGACCTGCGACT
GATGATTAAACTCCACAGCAACGACACCGGTAATTCAGGTTTCATGGAGGGGGTCACCCTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

59.943

95.135

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Acinetobacter baumannii D1279779

40.643

92.432

0.376
  pilT Acinetobacter baumannii strain A118

40.643

92.432

0.376
  pilT Acinetobacter nosocomialis M2

40.643

92.432

0.376
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362

Multiple sequence alignment