Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q4S45_RS01315 Genome accession   NZ_CP131935
Coordinates   290502..291545 (-) Length   347 a.a.
NCBI ID   WP_305508413.1    Uniprot ID   A0AAT9WGU7
Organism   Massilia sp. R2A-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 285502..296545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q4S45_RS01300 (Q4S45_01300) - 286723..288582 (-) 1860 WP_305508407.1 ATP-binding protein -
  Q4S45_RS01305 (Q4S45_01305) - 288720..289202 (-) 483 WP_305508409.1 glutathione peroxidase -
  Q4S45_RS01310 (Q4S45_01310) pilU 289350..290486 (-) 1137 WP_305508411.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q4S45_RS01315 (Q4S45_01315) pilT 290502..291545 (-) 1044 WP_305508413.1 type IV pilus twitching motility protein PilT Machinery gene
  Q4S45_RS01320 (Q4S45_01320) - 291637..292353 (+) 717 WP_305508415.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q4S45_RS01325 (Q4S45_01325) proC 292350..293168 (+) 819 WP_305508417.1 pyrroline-5-carboxylate reductase -
  Q4S45_RS01330 (Q4S45_01330) - 293252..293500 (-) 249 WP_305508419.1 hypothetical protein -
  Q4S45_RS01335 (Q4S45_01335) - 293487..293870 (-) 384 WP_305508421.1 phage holin family protein -
  Q4S45_RS01340 (Q4S45_01340) - 293874..294197 (-) 324 WP_305508423.1 YqjD family protein -
  Q4S45_RS01345 (Q4S45_01345) ubiA 294257..295111 (-) 855 WP_305508425.1 4-hydroxybenzoate octaprenyltransferase -
  Q4S45_RS01350 (Q4S45_01350) - 295185..295517 (-) 333 WP_305508427.1 nucleotidyltransferase family protein -
  Q4S45_RS01355 (Q4S45_01355) - 295517..295939 (-) 423 WP_305508429.1 nucleotidyltransferase substrate binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37988.66 Da        Isoelectric Point: 6.7238

>NTDB_id=866370 Q4S45_RS01315 WP_305508413.1 290502..291545(-) (pilT) [Massilia sp. R2A-15]
MDISELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKQYEEVLECDFSFAIPGLAR
FRVNAFNQERGAAAVLRTIPSKILSLEDLNAPKIFAELAMKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHDSKKCLINQREVGPHTLSFNNALRSALREDPDCILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPADEKEMVRAMLSESLQAVVSQTLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLVKRNIISLGAARNAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=866370 Q4S45_RS01315 WP_305508413.1 290502..291545(-) (pilT) [Massilia sp. R2A-15]
ATGGACATCTCCGAATTACTCGCTTTCTCCGTCAAGAACAAGGCTTCCGACCTGCACCTGTCGTCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTCGAGCATAAGGACGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGCAATACGAGGAAGTGCTCGAATGCGACTTCTCGTTCGCCATCCCGGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGGAACGCGGCGCCGCCGCCGTGCTGCGCACGATTCCGTCGAAGATCCTGTCGCTCGA
AGACCTGAACGCGCCGAAGATTTTCGCCGAACTGGCGATGAAGCCGCGTGGCCTGGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAATCGACCACGCTGGCGGCGATGGTCAATCACCTCAACGAAAACGAGTACGGCCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTGCACGACTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGACTGCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGGCTTG
CCCTGTCGGCCGCCGAAACCGGCCACCTGGTATTCGGCACGCTGCATACCTCGTCGGCGGCCAAGACCATCGATCGTATC
GTCGACGTGTTCCCGGCCGACGAAAAGGAAATGGTGCGCGCGATGCTGTCCGAATCGCTGCAGGCGGTGGTCTCGCAGAC
CCTGCTGAAGACCAAGGACGGCTCCGGCCGCGTGGCCGCGCACGAGATCATGATCGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAAGCGAAGATCGCGCAGATGTATTCGGCGATCCAGACCGGCAGCAACGTCGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTCAAGCGTAATATCATCTCGCTTGGCGCCGCGCGTAACGCCGCCAAGACGCCGGATAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

76.106

97.695

0.744
  pilT Legionella pneumophila strain ERS1305867

76.106

97.695

0.744
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.788

95.101

0.654
  pilT Vibrio cholerae strain A1552

68.788

95.101

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Acinetobacter baylyi ADP1

42.486

99.712

0.424
  pilU Pseudomonas stutzeri DSM 10701

43.21

93.372

0.403
  pilU Vibrio cholerae strain A1552

39.71

99.424

0.395