Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q4S45_RS01310 Genome accession   NZ_CP131935
Coordinates   289350..290486 (-) Length   378 a.a.
NCBI ID   WP_305508411.1    Uniprot ID   A0AAT9WGH6
Organism   Massilia sp. R2A-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 284350..295486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q4S45_RS01290 (Q4S45_01290) glcE 284410..285462 (+) 1053 WP_305508399.1 glycolate oxidase subunit GlcE -
  Q4S45_RS01295 (Q4S45_01295) glcF 285462..286682 (+) 1221 WP_305508405.1 glycolate oxidase subunit GlcF -
  Q4S45_RS01300 (Q4S45_01300) - 286723..288582 (-) 1860 WP_305508407.1 ATP-binding protein -
  Q4S45_RS01305 (Q4S45_01305) - 288720..289202 (-) 483 WP_305508409.1 glutathione peroxidase -
  Q4S45_RS01310 (Q4S45_01310) pilU 289350..290486 (-) 1137 WP_305508411.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q4S45_RS01315 (Q4S45_01315) pilT 290502..291545 (-) 1044 WP_305508413.1 type IV pilus twitching motility protein PilT Machinery gene
  Q4S45_RS01320 (Q4S45_01320) - 291637..292353 (+) 717 WP_305508415.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q4S45_RS01325 (Q4S45_01325) proC 292350..293168 (+) 819 WP_305508417.1 pyrroline-5-carboxylate reductase -
  Q4S45_RS01330 (Q4S45_01330) - 293252..293500 (-) 249 WP_305508419.1 hypothetical protein -
  Q4S45_RS01335 (Q4S45_01335) - 293487..293870 (-) 384 WP_305508421.1 phage holin family protein -
  Q4S45_RS01340 (Q4S45_01340) - 293874..294197 (-) 324 WP_305508423.1 YqjD family protein -
  Q4S45_RS01345 (Q4S45_01345) ubiA 294257..295111 (-) 855 WP_305508425.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41650.04 Da        Isoelectric Point: 6.5773

>NTDB_id=866369 Q4S45_RS01310 WP_305508411.1 289350..290486(-) (pilU) [Massilia sp. R2A-15]
MERDQASKFMFDLLRLMTSKGGSDLFITAGFPPAIKIDGKVTPVSSQALTAAHTADLARSIMNDKQTAGFELTKEANFAI
SPGDLGRFRVSAFVQMSCVGMVLRVITTTIPKLEDLELPPVLADIIMAKRGLVIMVGATGSGKSTTMAAMVGYRNENSHG
HIITIEDPVEYVHPHKNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSS
NQAIDRIINFFPEERRAQLLMDLSLNLKGMVSQRLIPRKESKGRVVAIEIMLNSPLISDLIFKGQVHEIKEIMKKSRELG
MQTFDQALFDLHEADKISYEDALRNADSVNDLRLAIKLEGKDAKNRDLSAGTQHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=866369 Q4S45_RS01310 WP_305508411.1 289350..290486(-) (pilU) [Massilia sp. R2A-15]
ATGGAACGCGATCAGGCTTCAAAATTCATGTTCGACCTGCTCCGGCTGATGACCAGCAAGGGCGGCTCGGACCTGTTCAT
CACCGCGGGCTTCCCGCCGGCGATCAAGATCGACGGCAAAGTGACGCCGGTGTCGAGCCAGGCGCTGACCGCGGCGCACA
CCGCCGACCTGGCCCGCTCGATCATGAACGACAAGCAGACCGCCGGCTTCGAACTGACCAAGGAAGCGAATTTCGCCATC
AGCCCGGGCGACCTGGGACGCTTCCGCGTTTCGGCCTTCGTGCAAATGAGCTGCGTGGGCATGGTGCTGCGCGTGATCAC
CACCACGATCCCGAAACTCGAAGACCTCGAGCTGCCGCCGGTGCTGGCCGACATTATCATGGCCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCGACCGGCTCGGGCAAATCGACGACGATGGCGGCGATGGTCGGCTACCGCAACGAGAACAGCCACGGC
CACATCATCACCATCGAAGACCCGGTCGAATACGTCCACCCGCACAAGAACTGCGTGATCACGCAGCGCGAAGTGGGCGT
GGACACCGACAGCTTCGAGGCGGCGCTGAAAAACTCGCTGCGCCAGGCGCCCGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAAACGATGGAGCATGCGATCGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCTCT
AACCAGGCAATCGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGCGCGCAGCTGCTGATGGATTTGTCGCTGAACCT
GAAGGGCATGGTGTCGCAGCGCCTGATTCCGCGCAAGGAGTCCAAGGGCCGCGTGGTGGCGATCGAAATCATGCTCAATT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGACCTGCACGAGGCCGACAAGATCAGCTATGAAGACGCGCTGCGCAATGCCGA
CTCGGTCAACGATTTACGCCTTGCGATAAAATTGGAAGGCAAGGACGCGAAAAACCGCGACCTGTCCGCCGGCACACAAC
ACCTGGGGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(84-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.157

96.032

0.587
  pilU Acinetobacter baylyi ADP1

58.333

92.063

0.537
  pilU Vibrio cholerae strain A1552

57.514

91.534

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.624

0.399
  pilT Pseudomonas aeruginosa PAK

42.09

88.624

0.373
  pilT Acinetobacter nosocomialis M2

42.598

87.566

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Acinetobacter baylyi ADP1

43.125

84.656

0.365
  pilT Acinetobacter baumannii D1279779

43.218

83.862

0.362
  pilT Acinetobacter baumannii strain A118

43.218

83.862

0.362
  pilT Legionella pneumophila strain Lp02

41.39

87.566

0.362
  pilT Legionella pneumophila strain ERS1305867

41.39

87.566

0.362
  pilT Vibrio cholerae strain A1552

43.492

83.333

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.492

83.333

0.362