Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5625_RS15330 Genome accession   NZ_AP024609
Coordinates   3395773..3396810 (-) Length   345 a.a.
NCBI ID   WP_025009461.1    Uniprot ID   A0A1S2TIJ8
Organism   Shewanella algae strain ATCC 49138     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3390773..3401810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5625_RS15305 (TUM3811_30270) - 3390842..3391576 (-) 735 WP_096141503.1 tryptophan 2,3-dioxygenase family protein -
  K5625_RS15310 (TUM3811_30280) hemH 3391748..3392761 (-) 1014 WP_107005680.1 ferrochelatase -
  K5625_RS15315 (TUM3811_30290) - 3392796..3393377 (-) 582 WP_083390266.1 glutathione peroxidase -
  K5625_RS15320 (TUM3811_30300) - 3393417..3394526 (-) 1110 WP_117200436.1 hypothetical protein -
  K5625_RS15325 (TUM3811_30310) pilU 3394651..3395763 (-) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5625_RS15330 (TUM3811_30320) pilT 3395773..3396810 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  K5625_RS15335 (TUM3811_30330) - 3396847..3397542 (+) 696 WP_025009462.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5625_RS15340 (TUM3811_30340) proC 3397675..3398493 (+) 819 WP_044733986.1 pyrroline-5-carboxylate reductase -
  K5625_RS15345 (TUM3811_30350) - 3398517..3399068 (+) 552 WP_044733985.1 YggT family protein -
  K5625_RS15350 (TUM3811_30360) yggU 3399068..3399355 (+) 288 WP_037467122.1 DUF167 family protein YggU -
  K5625_RS15355 (TUM3811_30370) - 3399418..3399852 (+) 435 WP_025887551.1 DUF4426 domain-containing protein -
  K5625_RS15360 (TUM3811_30380) rdgB 3400072..3400674 (+) 603 WP_025009467.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K5625_RS15365 (TUM3811_30390) hemW 3400674..3401810 (+) 1137 WP_096141507.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.97 Da        Isoelectric Point: 6.8739

>NTDB_id=86492 K5625_RS15330 WP_025009461.1 3395773..3396810(-) (pilT) [Shewanella algae strain ATCC 49138]
MEITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINVPALDHQGVHRLVYDIMNDKQRKDYEEFLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILTLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QNLVNRGLISREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=86492 K5625_RS15330 WP_025009461.1 3395773..3396810(-) (pilT) [Shewanella algae strain ATCC 49138]
ATGGAAATCACTGAATTATTGGCCTTTAGTGTAAAGCATAACGCTTCGGATCTGCACCTGTCGGCCGGTGTGCCTCCCAT
GATACGCGTCGACGGCGAGGTCAGAAAGATTAATGTCCCTGCGCTTGATCACCAAGGGGTTCACAGACTTGTCTACGACA
TAATGAATGACAAGCAGCGTAAGGATTATGAAGAGTTTCTGGAAATCGACTTTTCCTTTGAAGTTCCCAATTTGGCGCGT
TTTCGTGTCAATGCCTTCAATCAGTCCCGCGGCGCCGCCGCGGTTTTTCGTACTATTCCCAGCGACATTTTGACTTTGGA
ACAACTGGGCGCGCCGGAAATCTTCAAGAAAATCGCTTCATATCCTCGCGGCCTGGTGCTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACCACCCTGGCGGCGATGATAGATTACGTCAATGAGAACCGTCACGATCATATTCTCACCATTGAAGAC
CCTATCGAATTCGTGCACCAAAACAAGCAATGCCTTATCAACCAGCGGGAAGTGCATCGCCATACCCACAGCTTTAATGC
CGCCCTCAGAAGCGCCCTGCGTGAAGACCCGGATGTCATCCTGGTCGGTGAGATGCGGGATTTGGAAACCATACGCCTGG
CGATGACCGCGGCAGAAACCGGCCACTTGGTGTTCGGTACCTTGCACACCACCTCGGCGGCCAAGACCATTGACCGTGTG
GTTGACGTATTCCCCGCAGGCGAGAAGGACATGGTGCGTACCATGTTGTCTGAGTCGTTGCAGGCGGTTATCTCCCAGAC
CCTGATCAAGAAAGTTGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGATGGGAACCCCGGCTATCCGAAACCTTATCC
GCGAGGACAAGGTGGCACAGATGTATTCTGCTATCCAAACCGGTATGGCCCATGGTATGCAAACCTTGGAGCAGAGCCTG
CAGAACCTGGTGAATCGCGGCTTGATCAGTCGGGAAGATGCCATGGCCAAGAGCTCCAACAAACAAGCGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2TIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.871

99.13

0.812
  pilT Acinetobacter baumannii D1279779

81.871

99.13

0.812
  pilT Acinetobacter baumannii strain A118

81.871

99.13

0.812
  pilT Acinetobacter baylyi ADP1

78.426

99.42

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment