Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5625_RS15325 Genome accession   NZ_AP024609
Coordinates   3394651..3395763 (-) Length   370 a.a.
NCBI ID   WP_025887558.1    Uniprot ID   A0A379YLK5
Organism   Shewanella algae strain ATCC 49138     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3389651..3400763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5625_RS15300 (TUM3811_30260) - 3389701..3390831 (-) 1131 WP_107110946.1 aminotransferase class V-fold PLP-dependent enzyme -
  K5625_RS15305 (TUM3811_30270) - 3390842..3391576 (-) 735 WP_096141503.1 tryptophan 2,3-dioxygenase family protein -
  K5625_RS15310 (TUM3811_30280) hemH 3391748..3392761 (-) 1014 WP_107005680.1 ferrochelatase -
  K5625_RS15315 (TUM3811_30290) - 3392796..3393377 (-) 582 WP_083390266.1 glutathione peroxidase -
  K5625_RS15320 (TUM3811_30300) - 3393417..3394526 (-) 1110 WP_117200436.1 hypothetical protein -
  K5625_RS15325 (TUM3811_30310) pilU 3394651..3395763 (-) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5625_RS15330 (TUM3811_30320) pilT 3395773..3396810 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  K5625_RS15335 (TUM3811_30330) - 3396847..3397542 (+) 696 WP_025009462.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5625_RS15340 (TUM3811_30340) proC 3397675..3398493 (+) 819 WP_044733986.1 pyrroline-5-carboxylate reductase -
  K5625_RS15345 (TUM3811_30350) - 3398517..3399068 (+) 552 WP_044733985.1 YggT family protein -
  K5625_RS15350 (TUM3811_30360) yggU 3399068..3399355 (+) 288 WP_037467122.1 DUF167 family protein YggU -
  K5625_RS15355 (TUM3811_30370) - 3399418..3399852 (+) 435 WP_025887551.1 DUF4426 domain-containing protein -
  K5625_RS15360 (TUM3811_30380) rdgB 3400072..3400674 (+) 603 WP_025009467.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3709

>NTDB_id=86491 K5625_RS15325 WP_025887558.1 3394651..3395763(-) (pilU) [Shewanella algae strain ATCC 49138]
MEVRPFLKAMVDRKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKDTMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=86491 K5625_RS15325 WP_025887558.1 3394651..3395763(-) (pilU) [Shewanella algae strain ATCC 49138]
ATGGAAGTCAGACCTTTTCTCAAAGCCATGGTGGATCGCAAGGCCTCGGATCTCTTTATTACCGCCGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGCTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAAGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGTATCGAAACCAAGATCCCTACTGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGACTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTAGGGGGAACCGGTA
CGGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCTAATGCCAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCACCAACAACTGCTATTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCAGCGGCCGGCGCGCCGCCATCGAAGTGCTGATCAACACCCCCAGGGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGACACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
TTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCCGACTCGCCAAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAATTCAGGTTTCATGGAGGGAGTTACCCTGGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379YLK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

59.375

95.135

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362

Multiple sequence alignment