Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KI216_RS03910 Genome accession   NZ_AP024595
Coordinates   806559..807596 (-) Length   345 a.a.
NCBI ID   WP_005011739.1    Uniprot ID   N9C916
Organism   Acinetobacter bouvetii strain JCM 18991     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801559..812596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI216_RS03880 (ACBO_07430) - 802333..802788 (+) 456 WP_005011758.1 bacteriohemerythrin -
  KI216_RS03885 (ACBO_07440) - 802933..804015 (+) 1083 WP_005011755.1 hypothetical protein -
  KI216_RS03890 (ACBO_07450) - 804015..804341 (+) 327 WP_005011748.1 RnfH family protein -
  KI216_RS03895 (ACBO_07460) - 804384..804782 (-) 399 WP_005011745.1 outer membrane protein assembly factor BamE -
  KI216_RS03900 (ACBO_07470) fur 804895..805332 (+) 438 WP_005011743.1 ferric iron uptake transcriptional regulator -
  KI216_RS03905 (ACBO_07480) pilU 805407..806528 (-) 1122 WP_005011741.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI216_RS03910 (ACBO_07490) pilT 806559..807596 (-) 1038 WP_005011739.1 type IV pilus twitching motility protein PilT Machinery gene
  KI216_RS03915 (ACBO_07500) - 807720..808412 (+) 693 WP_005011736.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI216_RS03920 (ACBO_07510) - 808700..809497 (+) 798 WP_005011733.1 thioesterase family protein -
  KI216_RS03925 (ACBO_07520) - 809661..810467 (+) 807 WP_005011728.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38228.89 Da        Isoelectric Point: 6.5062

>NTDB_id=86303 KI216_RS03910 WP_005011739.1 806559..807596(-) (pilT) [Acinetobacter bouvetii strain JCM 18991]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMMDYINDNRYDHILTVED
PIEFVHESKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KGLVSKGIISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=86303 KI216_RS03910 WP_005011739.1 806559..807596(-) (pilT) [Acinetobacter bouvetii strain JCM 18991]
ATGGACATTACAGAATTACTGGCATTTTCGGCCAAAAATGGTGCATCCGATTTGCACTTATCGGCAGGCATGCCGCCGCT
GATCCGTGTGGATGGCGAAGTCCGCCGCATTAATTTACCGGCTTTGGAACATAAAGAAGTTCACAAACTGGTCTACGACA
TTATGAATGATAAACAGCGCCGGGACTTTGAAGAAAAGCTGGAAACAGACTTTTCTTTTGAAGTGCCCGGTGTTGCCCGT
TTCCGTGTCAACGCATTCAACCAAAACCGCGGCGCAGGCGCGGTATTCCGGACCATTCCATCAAAAGTCCTTTCTATGGA
AGACTTAGGCATGGGCCAAATCTTTAAGGATATCTGTGAATATCCGCGCGGCATTGTGCTGGTTACAGGGCCAACGGGTT
CCGGTAAATCAACCACCCTGGCAGCAATGATGGACTATATCAACGACAACCGTTATGACCATATTCTAACCGTCGAAGAT
CCTATCGAATTTGTGCATGAATCAAAAAAATGCCTGATCAACCAGCGTGAAGTCCATCGCGATACCCATGGGTTTAACGA
AGCGCTCCGTTCAGCATTGCGTGAAGACCCCGATATTATCCTGGTCGGCGAGATGCGTGACCTTGAAACGATCCGTTTGG
CTTTAACAGCCGCAGAAACAGGCCACTTGGTCTTCGGCACCCTGCATACCACCTCTGCTGCCAAGACCATTGACCGTGTG
ATTGACGTTTTCCCAGCTGAAGAAAAAGATATGGTTCGCGCCATGCTGTCAGAATCCTTACAGGCGGTTATTTCACAAAC
ACTGCTGAAGAAAAATGGCGGCGGGCGTGTCGCTGCGCATGAAATCATGATCGGTATTCCAGCCATCCGCAACTTGATTC
GTGAAAACAAAGTGGCGCAAATGTATTCGGCCATTCAGACAGGTGCAAACTACGGCATGACGACGCTGGATCAAAGCTTA
AAAGGCTTAGTCTCTAAAGGCATCATCAGCCCGCAAGTTGCGCGTACAGCTGCAAAACAGCCTGAATCTTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9C916

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.362

100

0.954
  pilT Acinetobacter baumannii D1279779

95.072

100

0.951
  pilT Acinetobacter baumannii strain A118

95.072

100

0.951
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

84.593

99.71

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.302

99.71

0.841
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.277

96.232

0.667
  pilT Vibrio cholerae strain A1552

69.277

96.232

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.181

95.942

0.386

Multiple sequence alignment