Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KI216_RS03905 Genome accession   NZ_AP024595
Coordinates   805407..806528 (-) Length   373 a.a.
NCBI ID   WP_005011741.1    Uniprot ID   N9C832
Organism   Acinetobacter bouvetii strain JCM 18991     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 800407..811528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI216_RS03875 (ACBO_07420) - 801196..802044 (+) 849 WP_005011760.1 class II glutamine amidotransferase -
  KI216_RS03880 (ACBO_07430) - 802333..802788 (+) 456 WP_005011758.1 bacteriohemerythrin -
  KI216_RS03885 (ACBO_07440) - 802933..804015 (+) 1083 WP_005011755.1 hypothetical protein -
  KI216_RS03890 (ACBO_07450) - 804015..804341 (+) 327 WP_005011748.1 RnfH family protein -
  KI216_RS03895 (ACBO_07460) - 804384..804782 (-) 399 WP_005011745.1 outer membrane protein assembly factor BamE -
  KI216_RS03900 (ACBO_07470) fur 804895..805332 (+) 438 WP_005011743.1 ferric iron uptake transcriptional regulator -
  KI216_RS03905 (ACBO_07480) pilU 805407..806528 (-) 1122 WP_005011741.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KI216_RS03910 (ACBO_07490) pilT 806559..807596 (-) 1038 WP_005011739.1 type IV pilus twitching motility protein PilT Machinery gene
  KI216_RS03915 (ACBO_07500) - 807720..808412 (+) 693 WP_005011736.1 YggS family pyridoxal phosphate-dependent enzyme -
  KI216_RS03920 (ACBO_07510) - 808700..809497 (+) 798 WP_005011733.1 thioesterase family protein -
  KI216_RS03925 (ACBO_07520) - 809661..810467 (+) 807 WP_005011728.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41674.83 Da        Isoelectric Point: 6.5791

>NTDB_id=86302 KI216_RS03905 WP_005011741.1 805407..806528(-) (pilU) [Acinetobacter bouvetii strain JCM 18991]
MDFNGLLDYMVEKKASDLFITADVEPSIKINGTIMPIGSTKLPGATVGQLLNSIMSDKQRKEFADTRECNFAISNKDKSS
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASIIGFRNENSHGHIITIE
DPIEFIHEHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLKAMVAQQLIPTPDGSSRRAAIEILINSPLISDLIRKGEVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=86302 KI216_RS03905 WP_005011741.1 805407..806528(-) (pilU) [Acinetobacter bouvetii strain JCM 18991]
ATGGACTTTAACGGCTTGCTCGACTACATGGTCGAGAAAAAAGCATCTGACTTATTTATCACCGCTGATGTAGAGCCCTC
AATTAAAATCAACGGCACCATTATGCCGATTGGCTCTACCAAGCTGCCAGGCGCAACAGTCGGCCAGCTGCTCAACTCCA
TCATGTCAGACAAGCAGCGCAAGGAGTTTGCGGATACACGCGAATGCAACTTTGCCATCAGCAACAAAGACAAATCCTCC
CGTTTCCGTGTCAGCGCATTCCAGCAGCGTGACCAGCCGGGCATGGTGCTGCGCCGCATTGAAACGGTCATTCCGACCAT
GGATGAATTGAAACTGCCGCCAATTTTAAAAGAATTGGCCATGACCAAACGCGGCATTATCATTTTTGTTGGCGCAACCG
GTACTGGTAAATCCACGTCACTGGCTTCAATCATTGGCTTCCGCAATGAAAATTCACATGGCCATATCATTACTATTGAA
GATCCAATTGAATTTATTCATGAGCATAAAGGCTGCATCATTACGCAGCGTGAAGTCGGCATTGATACAGATTCATTTGA
AATCGCGCTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATTGGCGAGATCCGTTCCCGTGAAACCATGGATT
ACGCAATTGCCTTTGCCGAGACCGGCCACTTGGTTTTTGCCACGCTGCATGCCAACAATGCCAACCAAGCGATTGACCGG
ATTATTCACTTCTTTGAAGCCGACCGCCATGGCCAATTATTCATGGATTTATCGCTGAATTTGAAAGCGATGGTGGCACA
GCAGCTGATTCCAACGCCAGACGGCAGCTCACGCCGCGCAGCCATTGAAATCCTCATCAACTCCCCGCTGATTTCAGACC
TGATCCGCAAGGGTGAAGTGCACGAAATTAAAGATTTGATGAAACGTTCGCGTGAATTGGGAATGCAAACGTTTGACCAA
GCGTTGTTTGATTTGTATAAAGCCAAGCAAATTACCTACAAAGATGCGCTCAAACATGCAGACTCACCCAACGACTTGCG
TCTACAAATCAAACTGTCTGAAGAAGGCGGTGACCGCCTGCTGCATGCCAGCTCAAATATTACGTTTGATGGTCAGTCCT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9C832

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

74.339

100

0.753
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

55.405

99.196

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

42.012

90.617

0.381
  pilT Legionella pneumophila strain ERS1305867

42.012

90.617

0.381
  pilT Acinetobacter nosocomialis M2

40.525

91.957

0.373
  pilT Acinetobacter baumannii strain A118

40.525

91.957

0.373
  pilT Acinetobacter baumannii D1279779

40.525

91.957

0.373
  pilT Acinetobacter baylyi ADP1

39.942

91.957

0.367
  pilT Vibrio cholerae strain A1552

39.031

94.102

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.102

0.367
  pilT Pseudomonas aeruginosa PAK

39.595

92.761

0.367

Multiple sequence alignment