Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q5Z11_RS05990 Genome accession   NZ_CP130832
Coordinates   1328119..1329156 (+) Length   345 a.a.
NCBI ID   WP_296251652.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 610A2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1323119..1334156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z11_RS05965 - 1323634..1324041 (-) 408 WP_303749129.1 hypothetical protein -
  Q5Z11_RS05970 - 1324125..1325525 (-) 1401 WP_303749130.1 ATP-binding cassette domain-containing protein -
  Q5Z11_RS05975 - 1325808..1326269 (-) 462 WP_303749131.1 DUF4426 domain-containing protein -
  Q5Z11_RS05980 proC 1326496..1327329 (-) 834 WP_303749132.1 pyrroline-5-carboxylate reductase -
  Q5Z11_RS05985 - 1327361..1328032 (-) 672 WP_303749133.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5Z11_RS05990 pilT 1328119..1329156 (+) 1038 WP_296251652.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5Z11_RS05995 pilU 1329269..1330399 (+) 1131 WP_303749134.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5Z11_RS06000 - 1330716..1331252 (-) 537 WP_303749135.1 DNA-3-methyladenine glycosylase I -
  Q5Z11_RS06005 - 1331330..1331896 (+) 567 WP_303749136.1 YqgE/AlgH family protein -
  Q5Z11_RS06010 ruvX 1331889..1332356 (+) 468 WP_303749137.1 Holliday junction resolvase RuvX -
  Q5Z11_RS06015 - 1332374..1333324 (+) 951 WP_303749138.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38538.20 Da        Isoelectric Point: 6.5781

>NTDB_id=861492 Q5Z11_RS05990 WP_296251652.1 1328119..1329156(+) (pilT) [Stenotrophomonas sp. 610A2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPIFRELIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKRVGGGRVASHEIMVATPAIRNLIREDKVAQMYSSIQTGQQVGMQTLDQNL
QDLVKRSLVTRNQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=861492 Q5Z11_RS05990 WP_296251652.1 1328119..1329156(+) (pilT) [Stenotrophomonas sp. 610A2]
ATGGATATCGCTGAACTTTTGGCGTTCTCGGTCAAGAACAAGGCCTCGGACCTCCACCTCTCCGCTGGCCTGCCGCCGAT
GATCCGCGTGGACGGTGACGTCCGTCGCATCAACATCCCGGCCCTGGACCACAAACAAGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGTCGTGATTACGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCTCGCTGGCGCGC
TTCCGTGTCAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCGGAAGTGCTGACGCTGGA
AGACCTGGCCTGCCCGCCGATCTTCCGTGAGTTGATCGAACAGCCGCAGGGTCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGTCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
AGCACTGCGTTCGGCCTTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGTGACCTTGAGACCATCCGCCTGG
CACTGACCGCGGCAGAAACCGGTCACCTGGTGTTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTCATCTCGCAGGC
ACTGCTCAAGCGCGTTGGTGGTGGCCGTGTGGCCTCGCACGAAATCATGGTGGCAACACCGGCTATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCCATCCAGACCGGCCAGCAGGTGGGCATGCAGACGCTGGACCAGAACCTG
CAGGATCTGGTCAAGCGCAGTCTGGTCACGCGCAACCAGGCCAAGGAATACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.581

98.261

0.762
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.948

95.362

0.696
  pilT Vibrio cholerae strain A1552

72.948

95.362

0.696
  pilT Neisseria meningitidis 8013

66.862

98.841

0.661
  pilT Neisseria gonorrhoeae MS11

66.569

98.841

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397