Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q5Z11_RS05995 Genome accession   NZ_CP130832
Coordinates   1329269..1330399 (+) Length   376 a.a.
NCBI ID   WP_303749134.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 610A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1324269..1335399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z11_RS05975 - 1325808..1326269 (-) 462 WP_303749131.1 DUF4426 domain-containing protein -
  Q5Z11_RS05980 proC 1326496..1327329 (-) 834 WP_303749132.1 pyrroline-5-carboxylate reductase -
  Q5Z11_RS05985 - 1327361..1328032 (-) 672 WP_303749133.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5Z11_RS05990 pilT 1328119..1329156 (+) 1038 WP_296251652.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5Z11_RS05995 pilU 1329269..1330399 (+) 1131 WP_303749134.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5Z11_RS06000 - 1330716..1331252 (-) 537 WP_303749135.1 DNA-3-methyladenine glycosylase I -
  Q5Z11_RS06005 - 1331330..1331896 (+) 567 WP_303749136.1 YqgE/AlgH family protein -
  Q5Z11_RS06010 ruvX 1331889..1332356 (+) 468 WP_303749137.1 Holliday junction resolvase RuvX -
  Q5Z11_RS06015 - 1332374..1333324 (+) 951 WP_303749138.1 aspartate carbamoyltransferase catalytic subunit -
  Q5Z11_RS06020 - 1333613..1334350 (-) 738 WP_303749139.1 tetratricopeptide repeat protein -
  Q5Z11_RS06025 - 1334428..1334691 (-) 264 WP_303749140.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41845.09 Da        Isoelectric Point: 6.7715

>NTDB_id=861493 Q5Z11_RS05995 WP_303749134.1 1329269..1330399(+) (pilU) [Stenotrophomonas sp. 610A2]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVNGKLSPITQTPLTAQQSRDMVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPVIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIVTQREVGIDTDSWEAALKNTLRQAPDVIMIGEIRTREGMDHAVAFAETGHLVLATLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPTPDGKSRRVAMEILLGTPLVQDYIRDGEVHKLKEVMKDSVQLGMKTF
DQSLFELYQAGEISYDDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=861493 Q5Z11_RS05995 WP_303749134.1 1329269..1330399(+) (pilU) [Stenotrophomonas sp. 610A2]
ATGAGCACTATTGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTTTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCAACGGCAAGCTGTCACCGATCACCCAGACACCGCTGACGGCGCAGCAAAGCCGCGACATGG
TGCTCAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAGAAAACCCACGAATGCAACTTCGCCATCGGCGTCTCCGGC
GTGGGTCGCTTCCGTGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAACCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCG
CCACCGGTACCGGCAAGTCGACCTCGCTGGCAGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCATGAAGGCTGCATCGTGACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAGCGGCGTTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCATGATCGGCGAAATCCGTACCCGCGAGGGCA
TGGATCACGCGGTTGCGTTCGCTGAAACCGGTCACCTGGTGCTGGCCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GATCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGTTGATGGATCTGTCGCTGAACCTGAAGGGTGTGGT
CGCGCAGCAGCTGGTGCCGACCCCGGACGGCAAGAGCCGCCGCGTGGCGATGGAAATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGATGGCGAAGTGCACAAGCTGAAGGAAGTGATGAAGGATTCCGTGCAGTTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGACGACGCCTTGCGTTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGTGATGCCAAGACCTTGTCGCAGGGTCTGGACGGCGTGGAGATTGCCG
AGGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.482

97.606

0.678
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

55.056

94.681

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

94.947

0.407
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362