Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q5Z10_RS05050 Genome accession   NZ_CP130831
Coordinates   1105263..1106300 (+) Length   345 a.a.
NCBI ID   WP_303638171.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 704A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1100263..1111300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z10_RS05025 soxR 1101534..1101968 (+) 435 WP_303638166.1 redox-sensitive transcriptional activator SoxR -
  Q5Z10_RS05030 - 1102008..1102610 (-) 603 WP_303638167.1 short chain dehydrogenase -
  Q5Z10_RS05035 - 1102709..1103584 (+) 876 WP_303638168.1 LysR family transcriptional regulator -
  Q5Z10_RS05040 proC 1103656..1104477 (-) 822 WP_303638169.1 pyrroline-5-carboxylate reductase -
  Q5Z10_RS05045 - 1104498..1105175 (-) 678 WP_303638170.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5Z10_RS05050 pilT 1105263..1106300 (+) 1038 WP_303638171.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5Z10_RS05055 pilU 1106433..1107527 (+) 1095 WP_303639139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5Z10_RS05060 - 1107713..1108696 (+) 984 WP_303638172.1 aldo/keto reductase -
  Q5Z10_RS05065 - 1108752..1109651 (-) 900 WP_303638173.1 LysR family transcriptional regulator -
  Q5Z10_RS05070 - 1109801..1110010 (+) 210 WP_303638174.1 DUF4287 domain-containing protein -
  Q5Z10_RS05075 - 1110138..1110845 (+) 708 WP_303638175.1 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38623.37 Da        Isoelectric Point: 6.6654

>NTDB_id=861470 Q5Z10_RS05050 WP_303638171.1 1105263..1106300(+) (pilT) [Stenotrophomonas sp. 704A1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQQGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=861470 Q5Z10_RS05050 WP_303638171.1 1105263..1106300(+) (pilT) [Stenotrophomonas sp. 704A1]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCATCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGATGTTCGCCGGATCAACATTCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGTGTCAATGCGTTCAACCAGAACCGTGGCGCGGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCAGCGATGATCGACTACATCAACAAGAACGAATACGGCCATATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
AGCGCTGCGGTCGGCGCTGCGCGAAGACCCCGACATCATCCTGGTCGGCGAACTGCGTGACCTGGAAACCATCCGTCTGG
CGCTGACCGCTGCCGAAACCGGCCATCTGGTGTTCGGTACGCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAACCAATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGTGGCCGTACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGCAGGGCATGATGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391