Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q5Z10_RS05055 Genome accession   NZ_CP130831
Coordinates   1106433..1107527 (+) Length   364 a.a.
NCBI ID   WP_303639139.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 704A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1101433..1112527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5Z10_RS05025 soxR 1101534..1101968 (+) 435 WP_303638166.1 redox-sensitive transcriptional activator SoxR -
  Q5Z10_RS05030 - 1102008..1102610 (-) 603 WP_303638167.1 short chain dehydrogenase -
  Q5Z10_RS05035 - 1102709..1103584 (+) 876 WP_303638168.1 LysR family transcriptional regulator -
  Q5Z10_RS05040 proC 1103656..1104477 (-) 822 WP_303638169.1 pyrroline-5-carboxylate reductase -
  Q5Z10_RS05045 - 1104498..1105175 (-) 678 WP_303638170.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5Z10_RS05050 pilT 1105263..1106300 (+) 1038 WP_303638171.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5Z10_RS05055 pilU 1106433..1107527 (+) 1095 WP_303639139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5Z10_RS05060 - 1107713..1108696 (+) 984 WP_303638172.1 aldo/keto reductase -
  Q5Z10_RS05065 - 1108752..1109651 (-) 900 WP_303638173.1 LysR family transcriptional regulator -
  Q5Z10_RS05070 - 1109801..1110010 (+) 210 WP_303638174.1 DUF4287 domain-containing protein -
  Q5Z10_RS05075 - 1110138..1110845 (+) 708 WP_303638175.1 YitT family protein -
  Q5Z10_RS05080 - 1110918..1111463 (-) 546 WP_303638176.1 DNA-3-methyladenine glycosylase I -
  Q5Z10_RS05085 - 1111593..1112159 (+) 567 WP_303638177.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40583.64 Da        Isoelectric Point: 6.7682

>NTDB_id=861471 Q5Z10_RS05055 WP_303639139.1 1106433..1107527(+) (pilU) [Stenotrophomonas sp. 704A1]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=861471 Q5Z10_RS05055 WP_303639139.1 1106433..1107527(+) (pilU) [Stenotrophomonas sp. 704A1]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACCGCAGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATTTCCCC
GATCACCCAGACGCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTGCTGAACGTGATGACGCCGGCACAGCGCGAAGAGT
TCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCTTGTCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGC
AACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAA
GACGCTGGCAATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCGACCGGCACCGGCAAATCGACTTCGCTGGCGGCGA
TGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGTGAAGTCGGCATCGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGC
CCCGGACGTGATCATGATCGGCGAAGTGCGCACCCGCGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACGCTGCATGCCAACAATGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGTTGTTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTTGCACAGCAGCTGGTGCCGTCCCCGGACGGGCGTTC
GCGCAAGGTGGCGATGGAAATCCTGCTGGGCACCCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGAATCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAG
ATCAGCTACGAAGACGCGCTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
TGCGCGCACCCTGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363