Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WCU01_RS02030 Genome accession   NZ_CP147587
Coordinates   415657..416334 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain GN06667     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410657..421334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU01_RS02015 (WCU01_02015) chiA 412015..414717 (+) 2703 WP_021538187.1 bifunctional chitinase/lysozyme -
  WCU01_RS02020 (WCU01_02020) bfd 414886..415080 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WCU01_RS02025 (WCU01_02025) bfr 415152..415628 (+) 477 WP_000675504.1 bacterioferritin -
  WCU01_RS02030 (WCU01_02030) pilD 415657..416334 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  WCU01_RS02035 (WCU01_02035) gspM 416334..416795 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  WCU01_RS02040 (WCU01_02040) gspL 416792..417955 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  WCU01_RS02045 (WCU01_02045) gspK 417970..418953 (-) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  WCU01_RS02050 (WCU01_02050) gspJ 418946..419533 (-) 588 WP_021524559.1 type II secretion system minor pseudopilin GspJ -
  WCU01_RS02055 (WCU01_02055) gspI 419526..419903 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  WCU01_RS02060 (WCU01_02060) gspH 419900..420409 (-) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  WCU01_RS02065 (WCU01_02065) gspG 420417..420854 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=861471 WCU01_RS02030 WP_000178175.1 415657..416334(-) (pilD) [Escherichia coli strain GN06667]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=861471 WCU01_RS02030 WP_000178175.1 415657..416334(-) (pilD) [Escherichia coli strain GN06667]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387