Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q5H80_RS12165 Genome accession   NZ_CP130748
Coordinates   2781259..2782296 (-) Length   345 a.a.
NCBI ID   WP_304564903.1    Uniprot ID   -
Organism   Vibrio sp. SNU_ST1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2776259..2787296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5H80_RS12140 (Q5H80_12140) rsmE 2777213..2777944 (+) 732 WP_304564901.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q5H80_RS12145 (Q5H80_12145) gshB 2777954..2778904 (+) 951 WP_017629553.1 glutathione synthase -
  Q5H80_RS12150 (Q5H80_12150) - 2779021..2779584 (+) 564 WP_102340779.1 YqgE/AlgH family protein -
  Q5H80_RS12155 (Q5H80_12155) ruvX 2779631..2780053 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  Q5H80_RS12160 (Q5H80_12160) pilU 2780135..2781244 (-) 1110 WP_304564902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5H80_RS12165 (Q5H80_12165) pilT 2781259..2782296 (-) 1038 WP_304564903.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5H80_RS12170 (Q5H80_12170) - 2782323..2783039 (+) 717 WP_304564904.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5H80_RS12175 (Q5H80_12175) proC 2783109..2783927 (+) 819 WP_304564905.1 pyrroline-5-carboxylate reductase -
  Q5H80_RS12180 (Q5H80_12180) - 2784004..2784561 (+) 558 WP_012604836.1 YggT family protein -
  Q5H80_RS12185 (Q5H80_12185) yggU 2784591..2784881 (+) 291 WP_304564906.1 DUF167 family protein YggU -
  Q5H80_RS12190 (Q5H80_12190) - 2784910..2785341 (+) 432 WP_304564907.1 DUF4426 domain-containing protein -
  Q5H80_RS12195 (Q5H80_12195) - 2785390..2785989 (+) 600 WP_304564908.1 XTP/dITP diphosphatase -
  Q5H80_RS12200 (Q5H80_12200) hemW 2785989..2787176 (+) 1188 WP_304564909.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38215.74 Da        Isoelectric Point: 6.0507

>NTDB_id=861310 Q5H80_RS12165 WP_304564903.1 2781259..2782296(-) (pilT) [Vibrio sp. SNU_ST1]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMSQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=861310 Q5H80_RS12165 WP_304564903.1 2781259..2782296(-) (pilT) [Vibrio sp. SNU_ST1]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCAGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTCCATCGTTTGGTTTTTGAGA
TCATGAGCGATTCACAGCGTGGTGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGA
TTCCGTGTTAATGCTTTTAACCAAGCTCGTGGGTGCGCAGCGGTCTTTCGAACCATCCCTGTAGAAATCCCAACGTTAGA
GCAGTTGGGCGCACCTGATATTTTTGAAAAAATTGCCAATTATGAAAAAGGCTTAGTGCTGGTGACGGGCCCTACGGGGT
CTGGTAAATCGACAACTTTGGCGGCGATGGTTGATTATGTTAACCGTAACCACAATAAGCACATCCTAACGATTGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTTGTTAACCAACGAGAGGTCCATCGTGATACCCACAGCTTTAAAGC
TGCGCTGCGCAGCGCGTTACGTGAAGACCCAGACGTAATTCTTGTAGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACGGCCGCAGAAACTGGTCACCTAGTTTTTGGTACTTTGCATACCAGCTCTGCAGCAAAAACCGTAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGATATGGTTCGTTCGATGCTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTCGGTGGTGGTCGTGTCGCTTGTCACGAAATCATGATGGCGACACCTGCAATCAGAAACTTGATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATTATTCAAACGGGCGCGGCACATGGTATGCAGACCATGGAGCAAAACGCG
AAACAGCTGATGTCGCAAGGCTTGGTTGACTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

88.116

100

0.881
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.116

100

0.881
  pilT Acinetobacter nosocomialis M2

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

68.235

98.551

0.672
  pilT Legionella pneumophila strain Lp02

65.797

100

0.658
  pilT Legionella pneumophila strain ERS1305867

65.797

100

0.658
  pilT Neisseria meningitidis 8013

63.478

100

0.635
  pilT Neisseria gonorrhoeae MS11

63.188

100

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.841

0.493
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368