Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q5H80_RS12160 Genome accession   NZ_CP130748
Coordinates   2780135..2781244 (-) Length   369 a.a.
NCBI ID   WP_304564902.1    Uniprot ID   -
Organism   Vibrio sp. SNU_ST1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2775135..2786244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5H80_RS12140 (Q5H80_12140) rsmE 2777213..2777944 (+) 732 WP_304564901.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q5H80_RS12145 (Q5H80_12145) gshB 2777954..2778904 (+) 951 WP_017629553.1 glutathione synthase -
  Q5H80_RS12150 (Q5H80_12150) - 2779021..2779584 (+) 564 WP_102340779.1 YqgE/AlgH family protein -
  Q5H80_RS12155 (Q5H80_12155) ruvX 2779631..2780053 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  Q5H80_RS12160 (Q5H80_12160) pilU 2780135..2781244 (-) 1110 WP_304564902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5H80_RS12165 (Q5H80_12165) pilT 2781259..2782296 (-) 1038 WP_304564903.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5H80_RS12170 (Q5H80_12170) - 2782323..2783039 (+) 717 WP_304564904.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5H80_RS12175 (Q5H80_12175) proC 2783109..2783927 (+) 819 WP_304564905.1 pyrroline-5-carboxylate reductase -
  Q5H80_RS12180 (Q5H80_12180) - 2784004..2784561 (+) 558 WP_012604836.1 YggT family protein -
  Q5H80_RS12185 (Q5H80_12185) yggU 2784591..2784881 (+) 291 WP_304564906.1 DUF167 family protein YggU -
  Q5H80_RS12190 (Q5H80_12190) - 2784910..2785341 (+) 432 WP_304564907.1 DUF4426 domain-containing protein -
  Q5H80_RS12195 (Q5H80_12195) - 2785390..2785989 (+) 600 WP_304564908.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41454.32 Da        Isoelectric Point: 5.6693

>NTDB_id=861309 Q5H80_RS12160 WP_304564902.1 2780135..2781244(-) (pilU) [Vibrio sp. SNU_ST1]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVLADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=861309 Q5H80_RS12160 WP_304564902.1 2780135..2781244(-) (pilU) [Vibrio sp. SNU_ST1]
ATGGAATTGAATCAAATTCTTGAAGGGATGCTTTCTCAAAAGGCGTCGGATCTTTACATCACTGTCGATGCGCCAATCCT
ATTTCGTGTGGATGGTGAACTGCGACCTCAGGGAGAGAAGTTGAATGCTGATCAAGTATCTCAGTTACTTGATGCAATGA
TGGATCAAGATCGACGCGATGAATACAAACAAACGCGTGAGGCTAATTTTGCCATCGTGCGTGATTTTGGTCGTTTTCGT
GTCAGTGCGTTCTTTCAGCGAGAGCTACCAGGAGCAGTAATTCGACGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGACGTACTACAAGACCTTTCAATCGCTAAGCGCGGGCTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGCA
AATCGACCTCGATGGCTGCGATGACAGGCTATCGCAATACCAATCGTTCAGGACATATCTTAACGGTTGAAGACCCAATT
GAATTCGTACACGAACATAAAAAGTGCATCGTGACTCAACGTGAGGTTGGACTCGACACCGAAAGCTATGAAGTCGCGCT
TAAAAACTCGTTACGCCAAGCCCCTGATATGATTTTGATTGGCGAAATCCGTAGTCGTGAAACCATGGAATACGCAATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCAACCCTGCATGCCAATAACGCTAACCAGGCGCTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAGCAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATCTGCGTGGTGTGGTTGCTCAGCAGTTAAT
ACGCGATAAAAATGGCAATGGTCGTCATGGTGTCTTCGAAATTCTGCTGAATAGCCCTCGTATATCTGACTTGATTCGTC
GTGGTGACTTGCATGAGTTAAAAGCGACTATGGCCAAATCGAAAGAGATTGGTATGCAGACTTTTGACCAAGCTTTGTAT
GATTTAGTGCTTGCGGACAAGATTAGCGAGGATGATGCGTTCCATAGTGCAGATTCTGCCAATGACTTGCGCTTAATGCT
TAAGACTAAACGTGGCGATGATGACTACGGAACTGGTGCGTTGGCTGGCGTGAAGATTGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.054

99.729

0.848
  pilU Pseudomonas stutzeri DSM 10701

55.256

100

0.556
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Legionella pneumophila strain ERS1305867

42.982

92.683

0.398
  pilT Legionella pneumophila strain Lp02

42.982

92.683

0.398
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

91.87

0.385
  pilT Pseudomonas aeruginosa PAK

41.298

91.87

0.379
  pilT Acinetobacter baylyi ADP1

42.638

88.347

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.597

90.786

0.369
  pilT Vibrio cholerae strain A1552

41.538

88.076

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.076

0.366