Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q2K57_RS06290 Genome accession   NZ_CP130701
Coordinates   1427020..1428051 (-) Length   343 a.a.
NCBI ID   WP_112055887.1    Uniprot ID   A0A328XSB2
Organism   Halomonas sp. I5-271120     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1422020..1433051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2K57_RS06275 tyrS 1423217..1424416 (-) 1200 WP_304526372.1 tyrosine--tRNA ligase -
  Q2K57_RS06280 - 1424553..1425710 (+) 1158 WP_304526373.1 anhydro-N-acetylmuramic acid kinase -
  Q2K57_RS06285 pilU 1425795..1426931 (-) 1137 WP_304526374.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q2K57_RS06290 pilT 1427020..1428051 (-) 1032 WP_112055887.1 type IV pilus twitching motility protein PilT Machinery gene
  Q2K57_RS06295 - 1428086..1428796 (+) 711 WP_304526375.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q2K57_RS06300 proC 1428935..1429759 (+) 825 WP_304526376.1 pyrroline-5-carboxylate reductase -
  Q2K57_RS06305 - 1429880..1430470 (+) 591 WP_304526377.1 YggT family protein -
  Q2K57_RS06310 - 1430573..1431724 (+) 1152 WP_304526378.1 homoserine O-acetyltransferase -
  Q2K57_RS06315 metW 1431724..1432326 (+) 603 WP_112055892.1 methionine biosynthesis protein MetW -
  Q2K57_RS06320 - 1432361..1432849 (+) 489 WP_304526379.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37409.98 Da        Isoelectric Point: 5.9357

>NTDB_id=861090 Q2K57_RS06290 WP_112055887.1 1427020..1428051(-) (pilT) [Halomonas sp. I5-271120]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDDREVRALIYDIMGDRQRRDFEEFLETDFSFEVPGVAR
FRVNAFQQGRGAAAVLRTVPAEVLTMEALGMGDVFRRMAQLPRGLVLVTGPTGSGKSTTLAAMIDFINDTRYDHILTIED
PVEFVHRSKRCLINQREVSRDTHGFAPALRSALREDPDVILVGELRDLDTIRLALTAAETGHLVLGTLHTTSAAKTIDRI
VDVFPGAEKDMVRSMLSESLQGVISQVLLKRAGGGRVAAHEILVATSAIRNLIREDKVAQIYSAIQTGGNLGMQTLDAAL
AKMVNDGVVVREEAQAVAKGVLS

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=861090 Q2K57_RS06290 WP_112055887.1 1427020..1428051(-) (pilT) [Halomonas sp. I5-271120]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAACGCATCGGACCTTCACCTTTCCGCCGGCTTGCCGCCGAT
GATTCGGGTCGATGGTGATATTCGCCGGCTCAACGTGCCAGCCATGGACGATCGTGAGGTGCGGGCCCTGATCTACGACA
TTATGGGCGACCGCCAGCGCCGCGACTTCGAAGAGTTCCTGGAAACGGACTTCTCCTTCGAGGTGCCGGGGGTGGCACGC
TTTCGCGTCAACGCATTCCAGCAGGGGCGCGGTGCCGCGGCGGTGCTGCGTACCGTGCCTGCGGAGGTGCTGACCATGGA
AGCGCTGGGCATGGGCGATGTCTTTCGCCGTATGGCGCAGCTTCCACGTGGCCTGGTACTGGTCACTGGCCCCACGGGGT
CGGGCAAGAGCACCACTCTGGCGGCGATGATCGACTTCATCAACGACACCCGCTACGACCATATCCTGACCATCGAAGAT
CCAGTGGAATTCGTGCACCGTAGCAAGCGTTGCCTGATCAACCAGCGTGAAGTGAGTCGCGATACTCACGGCTTCGCTCC
TGCGCTGCGCAGCGCCCTGCGCGAGGACCCGGACGTTATCCTGGTCGGCGAGCTTCGGGACCTGGATACCATCCGGCTGG
CGCTGACCGCCGCCGAGACCGGGCACCTAGTGCTGGGCACCCTGCACACCACGTCGGCAGCCAAGACCATCGACCGCATC
GTCGACGTCTTCCCCGGGGCGGAGAAGGATATGGTGCGCTCGATGCTCTCGGAGTCGCTGCAGGGCGTGATTTCGCAGGT
GCTGCTCAAGCGTGCCGGCGGTGGCCGGGTGGCGGCGCACGAGATCCTGGTGGCGACCTCGGCGATTCGCAACCTGATTC
GCGAGGACAAGGTGGCCCAAATCTATTCGGCCATCCAGACCGGCGGCAACCTGGGTATGCAGACGCTGGATGCGGCCCTG
GCCAAGATGGTCAACGATGGGGTTGTGGTGCGTGAAGAGGCGCAGGCCGTGGCCAAGGGCGTGCTCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A328XSB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.876

98.834

0.77
  pilT Pseudomonas aeruginosa PAK

77.876

98.834

0.77
  pilT Acinetobacter nosocomialis M2

74.041

98.834

0.732
  pilT Acinetobacter baumannii D1279779

74.041

98.834

0.732
  pilT Acinetobacter baumannii strain A118

74.041

98.834

0.732
  pilT Acinetobacter baylyi ADP1

73.746

98.834

0.729
  pilT Legionella pneumophila strain Lp02

68.343

98.542

0.673
  pilT Legionella pneumophila strain ERS1305867

68.343

98.542

0.673
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.262

97.959

0.659
  pilT Vibrio cholerae strain A1552

67.262

97.959

0.659
  pilT Neisseria meningitidis 8013

64.516

99.417

0.641
  pilT Neisseria gonorrhoeae MS11

64.223

99.417

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.321

94.461

0.513
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.668

0.394
  pilU Vibrio cholerae strain A1552

39.701

97.668

0.388
  pilU Acinetobacter baylyi ADP1

39.13

93.878

0.367