Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q3Y66_RS01640 Genome accession   NZ_CP130610
Coordinates   338532..339575 (+) Length   347 a.a.
NCBI ID   WP_008958822.1    Uniprot ID   G4F969
Organism   Halomonas sp. HAL1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333532..344575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q3Y66_RS01610 (Q3Y66_01610) - 333893..334342 (-) 450 WP_008958816.1 DUF4426 domain-containing protein -
  Q3Y66_RS01615 (Q3Y66_01615) metW 334382..334975 (-) 594 WP_008958817.1 methionine biosynthesis protein MetW -
  Q3Y66_RS01620 (Q3Y66_01620) - 334975..336171 (-) 1197 WP_008958818.1 homoserine O-acetyltransferase -
  Q3Y66_RS01625 (Q3Y66_01625) - 336241..336834 (-) 594 WP_008958819.1 YggT family protein -
  Q3Y66_RS01630 (Q3Y66_01630) proC 336904..337728 (-) 825 WP_008958820.1 pyrroline-5-carboxylate reductase -
  Q3Y66_RS01635 (Q3Y66_01635) - 337783..338499 (-) 717 WP_008958821.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q3Y66_RS01640 (Q3Y66_01640) pilT 338532..339575 (+) 1044 WP_008958822.1 type IV pilus twitching motility protein PilT Machinery gene
  Q3Y66_RS01645 (Q3Y66_01645) pilU 339568..340743 (+) 1176 WP_008958823.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q3Y66_RS01650 (Q3Y66_01650) - 340766..341905 (-) 1140 WP_008958824.1 anhydro-N-acetylmuramic acid kinase -
  Q3Y66_RS01655 (Q3Y66_01655) tyrS 342051..343250 (+) 1200 WP_008958825.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38180.63 Da        Isoelectric Point: 6.0078

>NTDB_id=860630 Q3Y66_RS01640 WP_008958822.1 338532..339575(+) (pilT) [Halomonas sp. HAL1]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYDIMNDRQRRDYEEHLEIDFSFEVPGVAR
FRVNAFTQARGAGAVFRTIPNQVLSMQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTVDRI
IDVFPGEEKAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQMYSSIQTGGSLGMQTLDAAL
SRLVKDGTVSLEEAQLSAKGTLSMKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=860630 Q3Y66_RS01640 WP_008958822.1 338532..339575(+) (pilT) [Halomonas sp. HAL1]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATACGTGTTGATGGCGACATTCGTCGGCTCAATGTGCCCGCTATCGATAATAACGATGTGCGCAAATTGATCTACGACA
TCATGAATGATCGACAGCGGCGCGACTACGAAGAGCACTTGGAAATTGATTTTTCCTTTGAAGTGCCGGGCGTTGCGCGC
TTTCGCGTGAATGCTTTCACTCAAGCGCGCGGTGCAGGGGCGGTGTTTCGCACCATCCCCAATCAAGTGCTCTCTATGCA
GACCTTGGGGCTTGGTGAGGTATTTGAGCGCTTAGCGATGTTGCCGCGTGGCTTGGTGCTGGTAACAGGCCCCACGGGGT
CGGGCAAGAGCACCACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTACGAGCACATTCTCACCATTGAAGAT
CCCGTTGAGTTTGTCCACGCCAGCAAGCGCTGTTTGATTAATCAACGCGAAGTGCACCGCGATACCCATAGCTTTGCCGA
TGCGCTGCGTAGCGCGCTGCGTGAAGACCCGGATGTGATTCTAGTCGGTGAGCTGCGTGATCTTGAAACTATCCGCTTGG
CGCTTACTGCGGCAGAGACGGGGCACCTGGTGCTTGGGACGCTACACACTACTTCGGCAGCTAAAACCGTTGACCGCATC
ATTGATGTCTTTCCCGGCGAAGAGAAAGCCATGGTGCGTTCGATGCTGTCCGAATCGCTCCAGGCGGTGGTGTCGCAATC
ACTGCTTAAACGCCAGGGAGGCGGACGTGTGGCGGCGCATGAAATCCTCATCGCCACTTCGGCAGTACGCAACTTGATTC
GCGAAGACAAAGTGGCGCAGATGTATTCGTCCATTCAAACCGGCGGCAGCCTGGGCATGCAAACCCTGGATGCCGCGCTG
TCACGGTTAGTGAAAGACGGTACGGTTAGTTTGGAAGAGGCGCAGCTGAGTGCGAAGGGAACGCTGTCGATGAAGGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4F969

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.876

97.695

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.876

97.695

0.761
  pilT Acinetobacter baylyi ADP1

74.419

99.135

0.738
  pilT Acinetobacter baumannii strain A118

75.516

97.695

0.738
  pilT Acinetobacter nosocomialis M2

75.516

97.695

0.738
  pilT Acinetobacter baumannii D1279779

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

72.189

97.406

0.703
  pilT Legionella pneumophila strain ERS1305867

72.189

97.406

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

96.542

0.677
  pilT Vibrio cholerae strain A1552

70.149

96.542

0.677
  pilT Neisseria meningitidis 8013

65.797

99.424

0.654
  pilT Neisseria gonorrhoeae MS11

65.507

99.424

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.521

93.948

0.522
  pilU Pseudomonas stutzeri DSM 10701

42.478

97.695

0.415
  pilU Vibrio cholerae strain A1552

41.593

97.695

0.406
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395