Detailed information NTG0860631

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q3Y66_RS01645
• Genome accession: NZ_CP130610
• Coordinates: 339568..340743 (+)
• Length: 391 a.a.
• NCBI ID: WP_008958823.1
• Uniprot ID: G4F970
• Organism: Halomonas sp. HAL1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 334568..345743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q3Y66_RS01620 (Q3Y66_01620)	-	334975..336171 (-)	1197	WP_008958818.1	homoserine O-acetyltransferase	-
Q3Y66_RS01625 (Q3Y66_01625)	-	336241..336834 (-)	594	WP_008958819.1	YggT family protein	-
Q3Y66_RS01630 (Q3Y66_01630)	proC	336904..337728 (-)	825	WP_008958820.1	pyrroline-5-carboxylate reductase	-
Q3Y66_RS01635 (Q3Y66_01635)	-	337783..338499 (-)	717	WP_008958821.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q3Y66_RS01640 (Q3Y66_01640)	pilT	338532..339575 (+)	1044	WP_008958822.1	type IV pilus twitching motility protein PilT	Machinery gene
Q3Y66_RS01645 (Q3Y66_01645)	pilU	339568..340743 (+)	1176	WP_008958823.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q3Y66_RS01650 (Q3Y66_01650)	-	340766..341905 (-)	1140	WP_008958824.1	anhydro-N-acetylmuramic acid kinase	-
Q3Y66_RS01655 (Q3Y66_01655)	tyrS	342051..343250 (+)	1200	WP_008958825.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43297.42 Da
• Isoelectric Point: 6.4624

>NTDB_id=860631 Q3Y66_RS01645 WP_008958823.1 339568..340743(+) (pilU) [Halomonas sp. HAL1]
MNKEHSVPDAQLSPTQWLHQLLDIMVEQNASDLLISVGAPPSLKMPDGLVPLGQQRLTAHQVNELVTHTLPEGLRERFAS
EHEANFALSREGKGRFRVSAFQQRNQMAMVVRSIAIEIPRLEALGVPEQLAELAQAKRGLVLVVGGTGTGKSTTLAAMIQ
QRNETVGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCL
ATLHANNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPTLAGGRRAAIEIMLQSPRIADLIRKGEVAEIKAAM
AKSRDAGMQTFDQALHDLHQAGHISREVAMAHADSANDLRMLLNVSDARARNLSLDEQVPSHLSLRDGDDY

Nucleotide

• Length: 1176 bp

>NTDB_id=860631 Q3Y66_RS01645 WP_008958823.1 339568..340743(+) (pilU) [Halomonas sp. HAL1]
ATGAATAAGGAACATAGCGTGCCAGATGCCCAACTTTCGCCCACCCAGTGGCTGCATCAACTACTCGATATTATGGTTGA
GCAGAACGCTTCTGATTTGCTCATCTCCGTGGGTGCGCCGCCAAGCCTGAAAATGCCCGATGGGCTGGTGCCGTTGGGTC
AGCAGCGTTTAACTGCCCATCAGGTCAACGAACTGGTCACCCACACGCTGCCGGAAGGGTTGCGCGAACGGTTTGCCAGT
GAGCATGAAGCGAACTTCGCGCTGAGTCGCGAGGGCAAAGGGCGTTTTCGGGTCAGCGCGTTTCAGCAGCGCAACCAGAT
GGCGATGGTGGTACGCAGCATCGCGATTGAAATTCCTCGCCTGGAGGCGCTAGGCGTGCCGGAGCAGTTGGCTGAATTAG
CTCAGGCCAAACGCGGGCTGGTACTGGTGGTAGGCGGTACGGGAACCGGCAAATCAACGACTCTGGCGGCGATGATTCAG
CAGCGCAACGAAACCGTCGGCGGTCATATCATCAGCATCGAAGACCCCATTGAGTACCTGCACCCGCACAAGCGTGCGAT
TGTGAATCAGCGGGAAGTGGGGATTGATACCGAGTCGTTTGAGGTGGCGCTTAAAAATACCCTGCGTCAGGCGCCGGATG
TGATTCTGATTGGTGAAATCCGCACCCGGGAAACCATGGAGCACGCGCTCACCTTCGCCGAAACCGGCCACCTCTGTTTA
GCCACCCTACATGCCAATAACGCCAATCAGGCGCTAGAGCGCATCATTCACTTCTTCCCTCATGAACGCCATGAGCAGAT
ATGGATGGATCTTTCGTTGAATCTGCGTGCGGTCGTTGCTCAGCAACTGCTGCCCACGCTTGCGGGTGGGCGCCGTGCAG
CCATTGAGATCATGCTGCAGTCGCCGCGCATTGCCGACTTGATCCGTAAAGGGGAAGTCGCCGAGATTAAAGCAGCGATG
GCCAAGTCCCGCGATGCTGGCATGCAGACCTTTGATCAGGCCCTGCATGACCTGCATCAGGCGGGGCATATCAGTAGAGA
GGTGGCCATGGCCCACGCCGACTCGGCTAACGACCTGCGCATGCTGCTCAACGTCAGTGACGCCAGAGCGCGTAACCTCT
CGCTGGACGAACAGGTGCCGAGTCATTTAAGCCTGCGCGATGGCGATGATTACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (83-285)

3D structure

• AlphaFold DB: G4F970

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.667	95.908	0.563
pilU	Vibrio cholerae strain A1552	58	89.514	0.519
pilU	Acinetobacter baylyi ADP1	55.966	90.026	0.504
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	93.095	0.379