Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LYSHEL_RS09355 Genome accession   NZ_AP024546
Coordinates   1922854..1923891 (+) Length   345 a.a.
NCBI ID   WP_213433757.1    Uniprot ID   -
Organism   Lysobacter helvus strain JCM32364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1917854..1928891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSHEL_RS09325 (LYSHEL_18360) - 1918745..1919287 (-) 543 WP_213433747.1 shikimate kinase -
  LYSHEL_RS09330 (LYSHEL_18370) pdxH 1919320..1919931 (+) 612 WP_213433749.1 pyridoxamine 5'-phosphate oxidase -
  LYSHEL_RS09335 (LYSHEL_18380) - 1919931..1920722 (+) 792 WP_213433751.1 cobalamin-binding protein -
  LYSHEL_RS09340 (LYSHEL_18390) - 1920726..1921202 (-) 477 WP_213433753.1 DUF4426 domain-containing protein -
  LYSHEL_RS09345 (LYSHEL_18400) proC 1921189..1922019 (-) 831 WP_213433754.1 pyrroline-5-carboxylate reductase -
  LYSHEL_RS09350 (LYSHEL_18410) - 1922030..1922701 (-) 672 WP_244858743.1 YggS family pyridoxal phosphate-dependent enzyme -
  LYSHEL_RS09355 (LYSHEL_18420) pilT 1922854..1923891 (+) 1038 WP_213433757.1 type IV pilus twitching motility protein PilT Machinery gene
  LYSHEL_RS09360 (LYSHEL_18430) pilU 1923908..1925038 (+) 1131 WP_213433758.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYSHEL_RS09365 (LYSHEL_18440) - 1925134..1925700 (+) 567 WP_213433759.1 YqgE/AlgH family protein -
  LYSHEL_RS09370 (LYSHEL_18450) ruvX 1925705..1926187 (+) 483 WP_213433760.1 Holliday junction resolvase RuvX -
  LYSHEL_RS09375 (LYSHEL_18460) - 1926184..1927131 (+) 948 WP_213433762.1 aspartate carbamoyltransferase catalytic subunit -
  LYSHEL_RS09380 (LYSHEL_18470) mgtE 1927243..1928604 (+) 1362 WP_213433764.1 magnesium transporter -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38504.28 Da        Isoelectric Point: 6.8285

>NTDB_id=85861 LYSHEL_RS09355 WP_213433757.1 1922854..1923891(+) (pilT) [Lysobacter helvus strain JCM32364]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTVPSEVLTLEDLGCPRIFKELIDQPQGLILVTGPTGSGKSTTLAAMLDHVNKSEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGEMRDIETIRLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGGAHGMMTLDQHL
QDLVKRSMVTRQHAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=85861 LYSHEL_RS09355 WP_213433757.1 1922854..1923891(+) (pilT) [Lysobacter helvus strain JCM32364]
ATGGATATCGCCGAACTCCTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTCCGGCGCATCAACATCCCCGCGCTCGACCACAAGCAGGTCCACGCGCTCGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAAGAATTCCTCGAGGTCGACTTCTCCTTCGAGATCCCGGGCCTGGCGCGC
TTCCGCGTGAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGCACGGTGCCCTCGGAGGTGCTGACGCTCGA
AGACCTCGGCTGCCCGCGCATCTTCAAGGAACTCATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCCACTGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGCTCGACCACGTCAACAAGAGCGAGTACGCGCACATCCTCTCGGTGGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCCCTGCGTTCGGCACTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAAATGCGCGACATCGAAACCATCCGCCTGG
CGCTCACCGCCGCGGAAACCGGCCACCTCGTGTTCGCGACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAAGTGGGCGGCGGTCGTACCGCGGCGTGGGAAATCATGGTCGGCATCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCGCGCACGGGATGATGACCCTCGACCAGCACCTG
CAGGACCTCGTGAAGCGCAGCATGGTCACGCGCCAGCACGCCAAGGAATACGCGAAGGACAAGCGGCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.552

97.101

0.675
  pilT Vibrio cholerae strain A1552

69.552

97.101

0.675
  pilT Neisseria meningitidis 8013

63.93

98.841

0.632
  pilT Neisseria gonorrhoeae MS11

63.636

98.841

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.484
  pilU Vibrio cholerae strain A1552

39.08

100

0.394
  pilU Pseudomonas stutzeri DSM 10701

39.823

98.261

0.391
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388

Multiple sequence alignment