Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LYSHEL_RS09360 Genome accession   NZ_AP024546
Coordinates   1923908..1925038 (+) Length   376 a.a.
NCBI ID   WP_213433758.1    Uniprot ID   -
Organism   Lysobacter helvus strain JCM32364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1918908..1930038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSHEL_RS09330 (LYSHEL_18370) pdxH 1919320..1919931 (+) 612 WP_213433749.1 pyridoxamine 5'-phosphate oxidase -
  LYSHEL_RS09335 (LYSHEL_18380) - 1919931..1920722 (+) 792 WP_213433751.1 cobalamin-binding protein -
  LYSHEL_RS09340 (LYSHEL_18390) - 1920726..1921202 (-) 477 WP_213433753.1 DUF4426 domain-containing protein -
  LYSHEL_RS09345 (LYSHEL_18400) proC 1921189..1922019 (-) 831 WP_213433754.1 pyrroline-5-carboxylate reductase -
  LYSHEL_RS09350 (LYSHEL_18410) - 1922030..1922701 (-) 672 WP_244858743.1 YggS family pyridoxal phosphate-dependent enzyme -
  LYSHEL_RS09355 (LYSHEL_18420) pilT 1922854..1923891 (+) 1038 WP_213433757.1 type IV pilus twitching motility protein PilT Machinery gene
  LYSHEL_RS09360 (LYSHEL_18430) pilU 1923908..1925038 (+) 1131 WP_213433758.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYSHEL_RS09365 (LYSHEL_18440) - 1925134..1925700 (+) 567 WP_213433759.1 YqgE/AlgH family protein -
  LYSHEL_RS09370 (LYSHEL_18450) ruvX 1925705..1926187 (+) 483 WP_213433760.1 Holliday junction resolvase RuvX -
  LYSHEL_RS09375 (LYSHEL_18460) - 1926184..1927131 (+) 948 WP_213433762.1 aspartate carbamoyltransferase catalytic subunit -
  LYSHEL_RS09380 (LYSHEL_18470) mgtE 1927243..1928604 (+) 1362 WP_213433764.1 magnesium transporter -
  LYSHEL_RS09385 (LYSHEL_18480) - 1928601..1929353 (+) 753 WP_213433766.1 tetratricopeptide repeat protein -
  LYSHEL_RS09390 (LYSHEL_18490) - 1929350..1929844 (+) 495 WP_213433768.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41878.15 Da        Isoelectric Point: 6.8286

>NTDB_id=85862 LYSHEL_RS09360 WP_213433758.1 1923908..1925038(+) (pilU) [Lysobacter helvus strain JCM32364]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKSRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEVAEAR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=85862 LYSHEL_RS09360 WP_213433758.1 1923908..1925038(+) (pilU) [Lysobacter helvus strain JCM32364]
ATGAGCAGCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACTTGTTCATCACGGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCCATCACCCAGAACCCGCTCACGCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGAACCCGCAGCAGCGGGAAGAGTTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTCGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CAGCGTCGAAGAGCTCAATTTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACGGGTACCGGTAAGTCCACGTCGCTGGCCGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACGCAGCGCGAAGTGGGCATCGACACCGACAG
CTGGGACGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACGCGCGAAGGCA
TGGACCACGCCATCGCCTTCGCCGAAACGGGCCACCTGGTGTTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGTCGCAACCAGTTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTCATCCCCACGCCCGACGGCAAGTCGCGCCGCGTGGCGATGGAAATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCTACGCCGACTCGGCGAACGA
AGTACGCCTGCGCATCAAGCTCGCCCAGGGCGGCGATGCGCGCACCTTGTCGCAGGGGCTGGATGGCGTCGAGGTCGCCG
AAGCCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

97.606

0.668
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.333

99.734

0.412
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Legionella pneumophila strain Lp02

40.896

89.096

0.364
  pilT Legionella pneumophila strain ERS1305867

40.896

89.096

0.364

Multiple sequence alignment