Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I0P84_RS11820 Genome accession   NZ_AP024524
Coordinates   2464142..2465263 (+) Length   373 a.a.
NCBI ID   WP_004783234.1    Uniprot ID   N9NI25
Organism   Acinetobacter variabilis strain RYU24     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2459142..2470263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0P84_RS11805 (RYU24_23490) - 2461124..2462230 (+) 1107 WP_214001513.1 acyltransferase family protein -
  I0P84_RS11810 (RYU24_23500) - 2462262..2462951 (-) 690 WP_214001514.1 YggS family pyridoxal phosphate-dependent enzyme -
  I0P84_RS11815 (RYU24_23510) pilT 2463075..2464112 (+) 1038 WP_004783232.1 type IV pilus twitching motility protein PilT Machinery gene
  I0P84_RS11820 (RYU24_23520) pilU 2464142..2465263 (+) 1122 WP_004783234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I0P84_RS11825 (RYU24_23530) fur 2465331..2465768 (-) 438 WP_004783237.1 ferric iron uptake transcriptional regulator -
  I0P84_RS11830 (RYU24_23540) - 2465881..2466279 (+) 399 WP_004783238.1 outer membrane protein assembly factor BamE -
  I0P84_RS11835 (RYU24_23550) - 2466383..2466712 (-) 330 WP_214001515.1 RnfH family protein -
  I0P84_RS11840 (RYU24_23560) - 2466712..2467791 (-) 1080 WP_214001516.1 hypothetical protein -
  I0P84_RS11845 (RYU24_23570) - 2467970..2468425 (-) 456 WP_214001517.1 bacteriohemerythrin -
  I0P84_RS11850 (RYU24_23580) - 2468721..2469569 (-) 849 WP_214002321.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41688.94 Da        Isoelectric Point: 6.8892

>NTDB_id=85815 I0P84_RS11820 WP_004783234.1 2464142..2465263(+) (pilU) [Acinetobacter variabilis strain RYU24]
MDFNGLLNYMVEKKASDLFITADVEPSIKINGQIVPIGSVKLPGAAVGQLLNSIMTEKQRKEFADTRECNFAISNPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGEHLLRAHSNITFDGQT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=85815 I0P84_RS11820 WP_004783234.1 2464142..2465263(+) (pilU) [Acinetobacter variabilis strain RYU24]
ATGGACTTTAACGGCTTACTCAACTACATGGTAGAGAAAAAGGCCTCGGATCTGTTCATTACAGCCGACGTTGAACCCTC
TATCAAAATTAACGGTCAGATTGTACCGATTGGCTCGGTGAAATTACCGGGCGCGGCTGTGGGGCAGTTACTTAACTCAA
TCATGACCGAGAAGCAGCGCAAGGAATTTGCCGACACACGCGAATGTAACTTCGCCATCAGCAACCCGGACAAATCAGCA
CGTTTCCGTGTCAGCGCATTCCAGCAACGCGATCAGCCAGGCATGGTACTGCGCCGTATTGAAACCGTCATTCCGACCAT
GGATGAGCTGAAGTTACCGCCAATTCTAAAAGAACTGGCAATGACCAAACGCGGAATCATTATTTTTGTGGGTGCAACCG
GTACTGGTAAATCGACTTCCCTCGCCTCTTTGATTGGTTACCGTAATGAAAATTCCAAAGGTCATATCATTACTATTGAA
GACCCGATCGAATTTGTGCACCAGCATAAGGGTTGTATCATTACTCAGCGCGAAGTCGGTATCGATACTGACTCCTTTGA
AGTCGCATTGAAAAACACCCTGCGTCAGGCACCAGATGTGATTCTGATCGGTGAGATCCGTTCTCGTGAAACCATGGACT
ATGCGATTGCCTTTGCGGAAACCGGTCACCTGGTTTTTGCGACCCTGCACGCCAACAACGCCAACCAGGCCATCGACCGT
ATCATTCACTTCTTCGAAGCCGACCGTCACAGCCAGTTGTTTATGGATCTGTCCCTGAACATGAAAGCCATTGTGGCACA
GCAGCTAATTCCAACTATCGATGGCAATGGCCGCCGCGCAGCGATCGAAATCCTGATCAATTCGCCATTGATTGCGGATC
TGATCCGTAAAGGCGATGTGCATGAAATCAAGGATTTGATGAAACGTTCACGTGAACTGGGCATGCAAACCTTCGACCAG
GCATTATATGACCTGTATAAGGCCAAGCAGATTAGTTATAAAGATGCACTAAAACATGCTGACTCACCAAACGACCTGCG
TCTGCAAATCAAACTATCAGAAGAAGGTGGCGAGCATTTATTACGTGCCCATTCCAATATTACTTTTGATGGTCAAACCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9NI25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.016

100

0.74
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.464

0.684
  pilU Vibrio cholerae strain A1552

57.835

94.102

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.775

92.761

0.397
  pilT Legionella pneumophila strain Lp02

43.155

90.08

0.389
  pilT Legionella pneumophila strain ERS1305867

43.155

90.08

0.389
  pilT Acinetobacter nosocomialis M2

41.983

91.957

0.386
  pilT Acinetobacter baumannii D1279779

41.983

91.957

0.386
  pilT Acinetobacter baumannii strain A118

41.983

91.957

0.386
  pilT Pseudomonas aeruginosa PAK

40.751

92.761

0.378
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Vibrio cholerae strain A1552

39.601

94.102

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.601

94.102

0.373
  pilT Neisseria meningitidis 8013

40.173

92.761

0.373
  pilT Neisseria gonorrhoeae MS11

40.173

92.761

0.373
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.761

0.367

Multiple sequence alignment