Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I0P84_RS11815 Genome accession   NZ_AP024524
Coordinates   2463075..2464112 (+) Length   345 a.a.
NCBI ID   WP_004783232.1    Uniprot ID   A0A2L0I6Y4
Organism   Acinetobacter variabilis strain RYU24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2458075..2469112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0P84_RS11805 (RYU24_23490) - 2461124..2462230 (+) 1107 WP_214001513.1 acyltransferase family protein -
  I0P84_RS11810 (RYU24_23500) - 2462262..2462951 (-) 690 WP_214001514.1 YggS family pyridoxal phosphate-dependent enzyme -
  I0P84_RS11815 (RYU24_23510) pilT 2463075..2464112 (+) 1038 WP_004783232.1 type IV pilus twitching motility protein PilT Machinery gene
  I0P84_RS11820 (RYU24_23520) pilU 2464142..2465263 (+) 1122 WP_004783234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I0P84_RS11825 (RYU24_23530) fur 2465331..2465768 (-) 438 WP_004783237.1 ferric iron uptake transcriptional regulator -
  I0P84_RS11830 (RYU24_23540) - 2465881..2466279 (+) 399 WP_004783238.1 outer membrane protein assembly factor BamE -
  I0P84_RS11835 (RYU24_23550) - 2466383..2466712 (-) 330 WP_214001515.1 RnfH family protein -
  I0P84_RS11840 (RYU24_23560) - 2466712..2467791 (-) 1080 WP_214001516.1 hypothetical protein -
  I0P84_RS11845 (RYU24_23570) - 2467970..2468425 (-) 456 WP_214001517.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38251.85 Da        Isoelectric Point: 6.5056

>NTDB_id=85814 I0P84_RS11815 WP_004783232.1 2463075..2464112(+) (pilT) [Acinetobacter variabilis strain RYU24]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGIISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=85814 I0P84_RS11815 WP_004783232.1 2463075..2464112(+) (pilT) [Acinetobacter variabilis strain RYU24]
ATGGATATTACAGAACTGTTAGCCTTTTCTGCTAAAAATGGTGCGTCGGATTTACACTTATCAGCAGGTTTACCACCCAT
GATTCGTGTGGACGGGGAAGTACGTCGCATCAACCTGCCTGCACTGGAACATAAAGAAGTACACAAACTGGTGTATGACA
TCATGAACGACAAGCAACGCCGTGATTATGAAGAAAATCTGGAAACTGACTTCTCTTTTGAAGTTCCAGGCGTGGCCCGT
TTCCGTGTGAACGCATTTAACCAGAACCGCGGTGCAGGCGCAGTATTCCGTACCATTCCATCTAAAGTACTGACCATTGA
AGATCTAGGTATGGGGCAGATCTTTAAAGATATCTGTGAATATCCACGGGGTATTGTACTGGTAACAGGACCAACCGGTT
CGGGTAAATCAACGACGCTTGCGGCAATGCTGGACTATATCAATGACAACCGCTATGACCATATCCTGACCGTCGAAGAT
CCAATCGAATTTGTACACCAATCGAAAAAATGTCTGATCAACCAGCGTGAAGTGCATCGTGACACGCATGGATTTAATGA
AGCATTACGTTCTGCATTACGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATCCGTCTGG
CACTCACTGCTGCGGAAACCGGTCATTTGGTCTTCGGTACCCTGCATACCACCTCAGCGGCAAAAACCATTGACCGTGTG
ATTGACGTGTTTCCTGCTGAAGAAAAAGACATGGTCCGTGCCATGTTGTCAGAATCTCTACAAGCCGTTATTTCACAAAC
CCTATTGAAGAAAAATGGGGGTGGTCGTGTGGCTGCACACGAAATCATGATCGGTATTCCGGCGATCCGTAACCTGATCC
GTGAAAATAAAGTGGCACAGATGTACTCTGCGATTCAAACCGGTGCCAACTATGGCATGACCACGCTGGATCAAAGCCTG
AAAACGCTGGTATCAAAAGGGATTATCAGTCCGCAAGTCGCCCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0I6Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.362

100

0.954
  pilT Acinetobacter baumannii strain A118

95.362

100

0.954
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

84.593

99.71

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.302

99.71

0.841
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.986

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment