Detailed information NTG0857774

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: WAZ52_RS18820
• Genome accession: NZ_CP147038
• Coordinates: 3782488..3783468 (+)
• Length: 326 a.a.
• NCBI ID: WP_001696140.1
• Uniprot ID: -
• Organism: Escherichia coli strain 21F61
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3777488..3788468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WAZ52_RS18790 (WAZ52_18790)	-	3777862..3778848 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
WAZ52_RS18795 (WAZ52_18795)	hemW	3779165..3780301 (-)	1137	WP_000239950.1	radical SAM family heme chaperone HemW	-
WAZ52_RS18800 (WAZ52_18800)	rdgB	3780294..3780887 (-)	594	WP_001174747.1	XTP/dITP diphosphatase	-
WAZ52_RS18805 (WAZ52_18805)	yggU	3780895..3781185 (-)	291	WP_001277194.1	DUF167 family protein YggU	-
WAZ52_RS18810 (WAZ52_18810)	yggT	3781182..3781748 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
WAZ52_RS18815 (WAZ52_18815)	yggS	3781766..3782470 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
WAZ52_RS18820 (WAZ52_18820)	pilT	3782488..3783468 (+)	981	WP_001696140.1	type IV pilus twitching motility protein PilT	Machinery gene
WAZ52_RS18825 (WAZ52_18825)	ruvX	3783642..3784058 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
WAZ52_RS18830 (WAZ52_18830)	yqgE	3784058..3784621 (-)	564	WP_001318029.1	YqgE/AlgH family protein	-
WAZ52_RS18835 (WAZ52_18835)	gshB	3784730..3785680 (-)	951	WP_042104644.1	glutathione synthase	-
WAZ52_RS18840 (WAZ52_18840)	rsmE	3785693..3786424 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
WAZ52_RS18845 (WAZ52_18845)	endA	3786504..3787211 (-)	708	WP_000286497.1	deoxyribonuclease I	-
WAZ52_RS18850 (WAZ52_18850)	yggI	3787306..3787803 (-)	498	WP_001696137.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35987.21 Da
• Isoelectric Point: 5.9975

>NTDB_id=857774 WAZ52_RS18820 WP_001696140.1 3782488..3783468(+) (pilT) [Escherichia coli strain 21F61]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPAVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=857774 WAZ52_RS18820 WP_001696140.1 3782488..3783468(+) (pilT) [Escherichia coli strain 21F61]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAGCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCAAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362