Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QZJ86_RS20700 Genome accession   NZ_CP129884
Coordinates   4470211..4471248 (+) Length   345 a.a.
NCBI ID   WP_301935477.1    Uniprot ID   -
Organism   Methylomonas montana strain MW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4465211..4476248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZJ86_RS20675 (QZJ86_20675) - 4465512..4465772 (+) 261 WP_301935472.1 hypothetical protein -
  QZJ86_RS20680 (QZJ86_20680) - 4465786..4467738 (-) 1953 WP_301935473.1 DUF3488 and DUF4129 domain-containing transglutaminase family protein -
  QZJ86_RS20685 (QZJ86_20685) - 4467741..4468691 (-) 951 WP_301935474.1 DUF58 domain-containing protein -
  QZJ86_RS20690 (QZJ86_20690) - 4468688..4469593 (-) 906 WP_301935475.1 MoxR family ATPase -
  QZJ86_RS20695 (QZJ86_20695) - 4469744..4470088 (+) 345 WP_301935476.1 TraR/DksA family transcriptional regulator -
  QZJ86_RS20700 (QZJ86_20700) pilT 4470211..4471248 (+) 1038 WP_301935477.1 type IV pilus twitching motility protein PilT Machinery gene
  QZJ86_RS20705 (QZJ86_20705) pilU 4471262..4472404 (+) 1143 WP_301935478.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QZJ86_RS20710 (QZJ86_20710) mltB 4472413..4473582 (+) 1170 WP_301935479.1 lytic murein transglycosylase B -
  QZJ86_RS20715 (QZJ86_20715) - 4474240..4474740 (+) 501 WP_301935480.1 hypothetical protein -
  QZJ86_RS20720 (QZJ86_20720) - 4474832..4475062 (-) 231 Protein_4092 transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.13 Da        Isoelectric Point: 7.2653

>NTDB_id=856205 QZJ86_RS20700 WP_301935477.1 4470211..4471248(+) (pilT) [Methylomonas montana strain MW1]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEDLNAPKFFAELCTKPRGLILVTGPTGSGKSTTLAAMVNHINNNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDRGQITAKAAMTRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=856205 QZJ86_RS20700 WP_301935477.1 4470211..4471248(+) (pilT) [Methylomonas montana strain MW1]
ATGGATATTGCCGAACTGTTGACCTTTTCCGTCAAAAACAAAGCCTCAGACTTGCATCTTTCCGCCGGCCTGCCACCGAT
GATTCGGGTGGATGGCGACATCCGCCGCATTAATATCCCGGCCTTGAATCACAAGGAAGTCCACGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGCCGTGATTACGAGGAATTTCTGGAAACCGACTTTTCCTTCGCCTTGCCCGGCGTGGCCCGT
TTCCGGGTGAACGCTTTCAATCAGGATAGAGGCGCCGGCGCGGTGTTCAGGACCATTCCCTCGAAAGTGCTGACGCTGGA
AGACCTGAACGCCCCCAAGTTTTTTGCGGAACTTTGCACAAAACCGCGCGGACTGATTCTGGTCACCGGCCCGACCGGTT
CCGGTAAATCGACCACACTGGCGGCGATGGTGAATCATATCAACAACAATGATTACGCCCATATTCTCACCGTCGAAGAT
CCCATCGAGTTTGTACACGAAAGCCAAAAATGCCTGATCAACCAGCGTGAAGTGCATAGAGACACGCTGGGCTTCAACGA
AGCCTTGCGCTCGGCTTTGCGGGAAGATCCTGACATCATTCTGGTCGGCGAGATGCGTGACCTGGAAACTATCCGGTTGG
CCCTGACCGCCGCAGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCACCTCGGCCGCGAAAACCATAGACCGGATC
ATCGACGTGTTTCCGGCCGCCGAAAAAGACATGATTCGGGCTATGCTGTCCGAGTCCCTGCAAGCGGTTATCTCGCAAAC
GCTTTTGAAAAAAGTCGGCGGTGGCCGGATCGCCGCGCACGAAATCATGGTCGGCACCCCGGCGATTCGCAACTTGATCC
GCGAAGCCAAGGTCGCGCAAATGTATTCGGCTATCCAAACCGGCCGCAAGGACGGTATGCAGACCCTGGATCAAAACCTG
AAGGAATTGGTCGATAGAGGCCAGATCACCGCTAAAGCCGCGATGACCAGAGCTGTCAACAAAGATATGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.105

99.71

0.809
  pilT Acinetobacter baumannii D1279779

81.105

99.71

0.809
  pilT Acinetobacter baumannii strain A118

81.105

99.71

0.809
  pilT Acinetobacter baylyi ADP1

80.938

98.841

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

79.882

97.971

0.783
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria gonorrhoeae MS11

66.181

99.42

0.658
  pilT Neisseria meningitidis 8013

66.181

99.42

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Acinetobacter baylyi ADP1

40.634

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.403
  pilU Vibrio cholerae strain A1552

40.413

98.261

0.397