Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QZJ86_RS20705 Genome accession   NZ_CP129884
Coordinates   4471262..4472404 (+) Length   380 a.a.
NCBI ID   WP_301935478.1    Uniprot ID   -
Organism   Methylomonas montana strain MW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4466262..4477404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZJ86_RS20685 (QZJ86_20685) - 4467741..4468691 (-) 951 WP_301935474.1 DUF58 domain-containing protein -
  QZJ86_RS20690 (QZJ86_20690) - 4468688..4469593 (-) 906 WP_301935475.1 MoxR family ATPase -
  QZJ86_RS20695 (QZJ86_20695) - 4469744..4470088 (+) 345 WP_301935476.1 TraR/DksA family transcriptional regulator -
  QZJ86_RS20700 (QZJ86_20700) pilT 4470211..4471248 (+) 1038 WP_301935477.1 type IV pilus twitching motility protein PilT Machinery gene
  QZJ86_RS20705 (QZJ86_20705) pilU 4471262..4472404 (+) 1143 WP_301935478.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QZJ86_RS20710 (QZJ86_20710) mltB 4472413..4473582 (+) 1170 WP_301935479.1 lytic murein transglycosylase B -
  QZJ86_RS20715 (QZJ86_20715) - 4474240..4474740 (+) 501 WP_301935480.1 hypothetical protein -
  QZJ86_RS20720 (QZJ86_20720) - 4474832..4475062 (-) 231 Protein_4092 transposase -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42676.95 Da        Isoelectric Point: 6.4438

>NTDB_id=856206 QZJ86_RS20705 WP_301935478.1 4471262..4472404(+) (pilU) [Methylomonas montana strain MW1]
MDFKALLALMVEKKASDLFITAGRPPSFKIDGRIVEVSKTLLTEEQTLALVNSIMSQRQRDEFENTKECQFALSVHELGR
FRVSAFTQRDAAGMVLRRIETTIPTSEDLHLPPVLKELIMQKRGLVIFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFMHPHKGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRETMQHAITFAETGHLCVCTLHANNANQALDRI
LHFFPDEMHGQLFMDLSLNLRGIVAQQLIKRADGKGRYPAIEILLNTPLVGDLIRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYVAGKISYDDALQSADSRNEVRLMIKLGAENAQFDNSEMKLTDDPDEGTNLYNTPR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=856206 QZJ86_RS20705 WP_301935478.1 4471262..4472404(+) (pilU) [Methylomonas montana strain MW1]
ATGGACTTTAAAGCCCTACTGGCCCTGATGGTTGAAAAGAAAGCATCCGACCTGTTCATTACCGCCGGCCGGCCGCCTTC
CTTTAAAATCGATGGCAGAATCGTCGAGGTCTCGAAAACGCTACTGACCGAGGAGCAGACCTTGGCTCTGGTGAATAGCA
TCATGTCGCAACGGCAACGCGACGAATTTGAGAATACCAAGGAATGTCAGTTTGCCTTGAGCGTCCATGAGCTGGGCCGG
TTCCGGGTCAGCGCGTTCACCCAGCGCGATGCGGCCGGCATGGTGCTGAGACGCATCGAAACCACTATTCCCACGTCTGA
AGATCTGCACTTGCCGCCGGTATTGAAAGAACTGATCATGCAAAAACGCGGCCTAGTCATCTTTGTCGGTGCGACCGGCA
CCGGTAAATCGACCTCGCTGGCTGCGTTGATCCGCCACCGCAACGAAAACAGTAGCGGCCATATCATCTCCATCGAAGAC
CCGATCGAGTTCATGCATCCGCACAAGGGTTGCATCATCACCCAGCGTGAAGTCGGACTGGACACCGAGTCGTTCGAAGT
CGCGCTGAAAAACACCCTGCGGCAAGCGCCGGACGTGATTCTGATCGGTGAGGTTAGAAGCCGGGAAACCATGCAGCACG
CCATTACCTTTGCCGAAACCGGCCATCTGTGCGTTTGCACCTTGCATGCCAATAACGCCAACCAGGCCTTGGACAGGATT
CTGCATTTCTTCCCGGACGAGATGCACGGCCAGTTGTTCATGGATTTATCGTTAAACCTGCGCGGCATCGTCGCGCAACA
GCTGATCAAGCGCGCCGACGGCAAGGGCCGCTATCCCGCCATCGAAATTCTGCTGAATACTCCCCTGGTAGGGGATTTGA
TCCGCAAGGGCGAAGTGCATAAGCTGAAAGAGTTGATGAAAAACTCCCGTGAGCACGGCATGCAGACTTTCGATCAGGCG
CTTTACGATCTGTATGTGGCCGGCAAAATCAGTTACGATGATGCGCTACAATCTGCCGATTCTCGTAACGAAGTCCGGTT
GATGATCAAACTTGGCGCCGAGAACGCTCAGTTCGACAACTCCGAAATGAAATTGACCGACGATCCTGACGAGGGCACCA
ATCTGTATAACACGCCGAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.774

93.158

0.65
  pilU Acinetobacter baylyi ADP1

64.835

95.789

0.621
  pilU Vibrio cholerae strain A1552

58.857

92.105

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.828

91.579

0.411
  pilT Legionella pneumophila strain Lp02

42.478

89.211

0.379
  pilT Legionella pneumophila strain ERS1305867

42.478

89.211

0.379
  pilT Pseudomonas aeruginosa PAK

41.298

89.211

0.368
  pilT Acinetobacter baumannii D1279779

41.298

89.211

0.368
  pilT Acinetobacter baumannii strain A118

41.298

89.211

0.368
  pilT Acinetobacter nosocomialis M2

41.298

89.211

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.708

89.211

0.363
  pilT Vibrio cholerae strain A1552

41.818

86.842

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

86.842

0.363
  pilT Acinetobacter baylyi ADP1

40.176

89.737

0.361