Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QYQ96_RS11430 Genome accession   NZ_CP129421
Coordinates   2517190..2518227 (-) Length   345 a.a.
NCBI ID   WP_055029008.1    Uniprot ID   A0A0Q0QI52
Organism   Vibrio metoecus strain ZF102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512190..2523227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ96_RS11405 (QYQ96_11405) - 2512685..2513248 (+) 564 WP_001054772.1 YqgE/AlgH family protein -
  QYQ96_RS11410 (QYQ96_11410) ruvX 2513316..2513738 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  QYQ96_RS11415 (QYQ96_11415) tyrS 2513764..2515044 (-) 1281 WP_304572214.1 tyrosine--tRNA ligase -
  QYQ96_RS11420 (QYQ96_11420) - 2515153..2515950 (-) 798 WP_304572215.1 helix-turn-helix transcriptional regulator -
  QYQ96_RS11425 (QYQ96_11425) pilU 2516069..2517175 (-) 1107 WP_304572216.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QYQ96_RS11430 (QYQ96_11430) pilT 2517190..2518227 (-) 1038 WP_055029008.1 type IV pilus twitching motility protein PilT Machinery gene
  QYQ96_RS11435 (QYQ96_11435) - 2518253..2518963 (+) 711 WP_055065167.1 YggS family pyridoxal phosphate-dependent enzyme -
  QYQ96_RS11440 (QYQ96_11440) proC 2519017..2519835 (+) 819 WP_304572217.1 pyrroline-5-carboxylate reductase -
  QYQ96_RS11445 (QYQ96_11445) - 2519890..2520447 (+) 558 WP_055065169.1 YggT family protein -
  QYQ96_RS11450 (QYQ96_11450) yggU 2520447..2520740 (+) 294 WP_055033223.1 DUF167 family protein YggU -
  QYQ96_RS11455 (QYQ96_11455) - 2520834..2521265 (+) 432 WP_001233671.1 DUF4426 domain-containing protein -
  QYQ96_RS11460 (QYQ96_11460) - 2521335..2521937 (+) 603 WP_304572218.1 XTP/dITP diphosphatase -
  QYQ96_RS11465 (QYQ96_11465) hemW 2521931..2523106 (+) 1176 WP_304572219.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38278.90 Da        Isoelectric Point: 6.5572

>NTDB_id=853163 QYQ96_RS11430 WP_055029008.1 2517190..2518227(-) (pilT) [Vibrio metoecus strain ZF102]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMVRGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=853163 QYQ96_RS11430 WP_055029008.1 2517190..2518227(-) (pilT) [Vibrio metoecus strain ZF102]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCCGCCGGTGTTCCGCCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGACGTACATCGCTTGGTATTTGAAA
TAATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGC
TTCCGGGTTAATGCGTTTCATCAAGCGCGCGGATGCTCTGCGGTGTTTCGTACCATTCCGACCGTGATCCCAACCTTAGA
GCAATTAGATGCACCTGAGATTTTTACCAAGATCGCCAATTATGAAAAAGGCTTAGTGTTGGTCACTGGACCTACCGGTT
CGGGTAAATCGACCACGCTCGCGGCGATGGTCGATTACGTGAATGCTCATCACAACAAGCATATCTTGACCATTGAAGAT
CCGATTGAATTTGTACACAGCAACAATAAGTGTTTGATCAACCAACGTGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGTTCGGCACTGCGTGAAGACCCCGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCGGAAACCGGTCACTTAGTGTTTGGTACACTGCACACCAGCTCGGCGGCAAAAACCATTGACCGGATT
ATCGATGTGTTTCCGGGCAGCGATAAAGACATGGTGCGTTCAATGCTGTCTGAATCGCTGCGTGCGGTTATCGCGCAAAA
GCTCTTAAAACGCGTGGGTGGCGGTCGTGTCGCGTGCCATGAAATCATGCTGGCGACTCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAATGCC
AAACAGCTGATGGTGCGTGGTGTGGTCGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0QI52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

98.551

100

0.986
  pilT Vibrio cholerae strain A1552

98.551

100

0.986
  pilT Acinetobacter nosocomialis M2

72.024

97.391

0.701
  pilT Acinetobacter baumannii D1279779

72.024

97.391

0.701
  pilT Acinetobacter baumannii strain A118

72.024

97.391

0.701
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

42.515

96.812

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374