Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QYQ96_RS11425 Genome accession   NZ_CP129421
Coordinates   2516069..2517175 (-) Length   368 a.a.
NCBI ID   WP_304572216.1    Uniprot ID   -
Organism   Vibrio metoecus strain ZF102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2511069..2522175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ96_RS11400 (QYQ96_11400) gshB 2511693..2512649 (+) 957 WP_304572211.1 glutathione synthase -
  QYQ96_RS11405 (QYQ96_11405) - 2512685..2513248 (+) 564 WP_001054772.1 YqgE/AlgH family protein -
  QYQ96_RS11410 (QYQ96_11410) ruvX 2513316..2513738 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  QYQ96_RS11415 (QYQ96_11415) tyrS 2513764..2515044 (-) 1281 WP_304572214.1 tyrosine--tRNA ligase -
  QYQ96_RS11420 (QYQ96_11420) - 2515153..2515950 (-) 798 WP_304572215.1 helix-turn-helix transcriptional regulator -
  QYQ96_RS11425 (QYQ96_11425) pilU 2516069..2517175 (-) 1107 WP_304572216.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QYQ96_RS11430 (QYQ96_11430) pilT 2517190..2518227 (-) 1038 WP_055029008.1 type IV pilus twitching motility protein PilT Machinery gene
  QYQ96_RS11435 (QYQ96_11435) - 2518253..2518963 (+) 711 WP_055065167.1 YggS family pyridoxal phosphate-dependent enzyme -
  QYQ96_RS11440 (QYQ96_11440) proC 2519017..2519835 (+) 819 WP_304572217.1 pyrroline-5-carboxylate reductase -
  QYQ96_RS11445 (QYQ96_11445) - 2519890..2520447 (+) 558 WP_055065169.1 YggT family protein -
  QYQ96_RS11450 (QYQ96_11450) yggU 2520447..2520740 (+) 294 WP_055033223.1 DUF167 family protein YggU -
  QYQ96_RS11455 (QYQ96_11455) - 2520834..2521265 (+) 432 WP_001233671.1 DUF4426 domain-containing protein -
  QYQ96_RS11460 (QYQ96_11460) - 2521335..2521937 (+) 603 WP_304572218.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41442.48 Da        Isoelectric Point: 6.8901

>NTDB_id=853162 QYQ96_RS11425 WP_304572216.1 2516069..2517175(-) (pilU) [Vibrio metoecus strain ZF102]
MELNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLADVTTLLNAMMDDARQAEFKQTREANFAVVRDRGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKNGKGRHGVFEVLLNSPRVSDLIRRGELHELKTTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=853162 QYQ96_RS11425 WP_304572216.1 2516069..2517175(-) (pilU) [Vibrio metoecus strain ZF102]
ATGGAGTTAAATCAATATCTGGATGGGATGCTCAGCCATAAAGCATCGGATCTTTACATCACCGTGGGAGCGCCGATTTT
ATATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGTACTCAGTTTGGCGGATGTCACCACCTTGTTGAATGCGATGA
TGGATGACGCGCGGCAAGCAGAATTTAAGCAGACGCGTGAAGCGAACTTTGCGGTGGTGCGTGACCGTGGCCGTTTTCGG
GTCAGCGCGTTTTTCCAACGTGAATTGCCGGGCGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTGCCTGAAGTGCTGCAAAATTTAGCGATCGCGAAACGCGGTTTAGTCTTAGTAGTGGGTGCTACGGGGTCAGGTA
AGTCGACCACTATGGCGGCAATGACGGGGTATCGAAATCAGCACCGTACCGGACATATCTTGACAGTTGAAGATCCGATT
GAGTTTGTGCATGAACACAAGCGCTGCATCGTGACTCAACGAGAAGTCGGGCTCGATACTGAAAGCTATGAAATCGCGCT
GAAAAACTCGCTGCGCCAAGCGCCGGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACTGGTCATTTGTGTATGGCCACTTTGCACGCCAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTCTTGCTCGACCTGTCACTCAACCTAAAAGGGGTGATTGGTCAGCAACTGTT
GCGCGATAAAAACGGCAAAGGACGACATGGAGTGTTTGAAGTGTTGCTCAATAGTCCCCGAGTCTCGGATTTGATTCGCC
GTGGCGAACTGCATGAGCTGAAAACGACCATGGCGCGCTCTAAAGAAGTCGGCATGCAGACTTTCGACCAAGCTTTGTAT
CAACTGGTGGTGGACGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCGGCTAATGATCTGCGCTTGATGCT
CAAAACTAAGCGTGGTGATAACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

96.467

100

0.965
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361