Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QYS50_RS12240 Genome accession   NZ_CP129415
Coordinates   2520805..2521995 (+) Length   396 a.a.
NCBI ID   WP_407569512.1    Uniprot ID   -
Organism   Deinococcus altitudinis strain LMG 24022     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2515805..2526995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYS50_RS12225 (QYS50_12220) - 2516456..2517274 (+) 819 WP_407569509.1 polyphenol oxidase family protein -
  QYS50_RS12230 (QYS50_12225) - 2517271..2517996 (+) 726 WP_407569510.1 YqeG family HAD IIIA-type phosphatase -
  QYS50_RS12235 (QYS50_12230) pilB 2517996..2520662 (+) 2667 WP_407569511.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  QYS50_RS12240 (QYS50_12235) pilT 2520805..2521995 (+) 1191 WP_407569512.1 type IV pilus twitching motility protein PilT Machinery gene
  QYS50_RS12245 (QYS50_12240) - 2522116..2522331 (+) 216 WP_407569513.1 hypothetical protein -
  QYS50_RS12250 (QYS50_12245) - 2522601..2523551 (+) 951 WP_407569514.1 GNAT family N-acetyltransferase -
  QYS50_RS12255 (QYS50_12250) - 2523535..2524233 (-) 699 WP_407569515.1 DsbA family protein -
  QYS50_RS12260 (QYS50_12255) - 2524524..2524835 (-) 312 WP_407569516.1 hypothetical protein -
  QYS50_RS12265 (QYS50_12260) - 2524922..2525584 (+) 663 WP_407569517.1 trimeric intracellular cation channel family protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43182.65 Da        Isoelectric Point: 7.2034

>NTDB_id=853126 QYS50_RS12240 WP_407569512.1 2520805..2521995(+) (pilT) [Deinococcus altitudinis strain LMG 24022]
MHVAIDITDILRIAAEKGASDVILTVGMPPQFKIHGVYGGQDFSELTATDTRKLMYSMMNEKQQRTFEEKRELDFSFALG
EKARFRVNTFMQKGNVGGVMRLIPTIIKSVSDLGLPPNVVDVANAPRGLVLVTGPTGSGKSTTLAAMLDYINVNKKMHIM
TIEDPIEFMHTHKNSIVNQREVGADTIDFQSALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRIDGGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMITM
DAYLAGLFRKRLITHEVGMERSVDSKEFARLASDPAAANALSPTLSGSMGMGGTPARPTANTATPASPSGNNYGRR

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=853126 QYS50_RS12240 WP_407569512.1 2520805..2521995(+) (pilT) [Deinococcus altitudinis strain LMG 24022]
ATGCACGTAGCCATCGACATCACCGACATCCTGCGAATTGCCGCCGAGAAGGGCGCCAGCGACGTGATCCTGACCGTCGG
CATGCCGCCCCAGTTCAAGATTCACGGCGTGTACGGCGGCCAGGATTTCTCCGAGCTGACCGCCACCGACACCCGCAAAC
TGATGTACAGCATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAAAAGCGCGAACTCGACTTCTCGTTCGCGCTCGGC
GAAAAGGCCCGCTTCCGTGTGAACACCTTCATGCAGAAGGGCAACGTGGGCGGCGTGATGCGCCTGATTCCCACCATCAT
CAAGTCGGTGAGCGACCTGGGCCTGCCGCCGAACGTGGTCGACGTCGCCAACGCGCCGCGCGGACTGGTGCTGGTGACCG
GGCCGACCGGATCGGGCAAATCGACCACCCTGGCCGCCATGCTCGACTACATCAACGTCAACAAGAAGATGCACATCATG
ACCATCGAAGACCCGATCGAGTTCATGCACACGCACAAGAACAGCATCGTCAACCAGCGTGAAGTAGGGGCCGACACCAT
CGATTTCCAGAGTGCGCTGCGAGCGGTGCTCAGGCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGACTACGAGA
CCATCAAGGCTGCCGTGACCGCCGCCGAGACCGGCCACCTGGTGATGGGCACGCTGCACACCAACAGCGCCCCCGAGAGC
ATCGACCGCATCGTGGACGTGTTTCCCGAAGAGCAGCAGGAGCAGATCCGGGTGCAGCTCGCCAACAACCTGGTGGCGGT
CATGACCCAGCAGCTGCTGCCGCGCATCGACGGCGGGCGCATCCTGGCCTACGAACTGCTGATCGCCAACCCGGCGGTGC
GCGCCCTGATCCGCGAGGGCAAGACCTACCAGATCACCAGCGTCATGCAGACCGGGGCGCGTGAGGGCATGATCACCATG
GACGCCTACCTCGCGGGGCTGTTCCGCAAGCGCCTGATCACGCACGAGGTCGGCATGGAGCGCTCGGTGGACAGCAAGGA
ATTCGCGCGCCTGGCCTCCGATCCGGCGGCGGCGAACGCCCTCAGCCCGACGCTGAGCGGCAGCATGGGCATGGGCGGCA
CGCCCGCACGGCCCACCGCCAACACCGCCACGCCCGCCTCCCCGAGCGGCAACAACTACGGACGGCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

74.559

100

0.747
  pilT Pseudomonas stutzeri DSM 10701

50.872

86.869

0.442
  pilT Pseudomonas aeruginosa PAK

51.775

85.354

0.442
  pilT Neisseria meningitidis 8013

50.289

87.374

0.439
  pilT Acinetobacter baumannii strain A118

52.727

83.333

0.439
  pilT Acinetobacter baumannii D1279779

52.727

83.333

0.439
  pilT Acinetobacter nosocomialis M2

52.727

83.333

0.439
  pilT Neisseria gonorrhoeae MS11

49.711

87.374

0.434
  pilT Acinetobacter baylyi ADP1

51.976

83.081

0.432
  pilT Legionella pneumophila strain ERS1305867

48.98

86.616

0.424
  pilT Legionella pneumophila strain Lp02

48.98

86.616

0.424
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.116

87.121

0.419
  pilT Vibrio cholerae strain A1552

48.116

87.121

0.419
  pilU Pseudomonas stutzeri DSM 10701

44.444

84.091

0.374
  pilU Acinetobacter baylyi ADP1

41.547

88.131

0.366
  pilU Vibrio cholerae strain A1552

43.114

84.343

0.364