Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JUNP479_RS09565 Genome accession   NZ_AP024466
Coordinates   2129115..2130149 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas jandaei strain JUNP479     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2124115..2135149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JUNP479_RS09535 (JUNP479_1937) glnK 2124749..2125087 (-) 339 WP_033114048.1 P-II family nitrogen regulator -
  JUNP479_RS09540 (JUNP479_1938) arfB 2125289..2125705 (+) 417 WP_033114047.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  JUNP479_RS09545 (JUNP479_1939) - 2125727..2126218 (+) 492 WP_203977566.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  JUNP479_RS09550 (JUNP479_1940) - 2126533..2126961 (-) 429 WP_033114045.1 hypothetical protein -
  JUNP479_RS09555 (JUNP479_1941) yaaA 2127156..2127929 (-) 774 WP_203977568.1 peroxide stress protein YaaA -
  JUNP479_RS09560 (JUNP479_1942) pilU 2127977..2129086 (-) 1110 WP_203977777.1 type IVa pilus ATPase TapU Machinery gene
  JUNP479_RS09565 (JUNP479_1943) pilT 2129115..2130149 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  JUNP479_RS09570 (JUNP479_1945) - 2130189..2130890 (+) 702 WP_041209073.1 YggS family pyridoxal phosphate-dependent enzyme -
  JUNP479_RS09575 (JUNP479_1946) proC 2130957..2131784 (+) 828 WP_203977570.1 pyrroline-5-carboxylate reductase -
  JUNP479_RS09580 (JUNP479_1947) - 2131796..2132347 (+) 552 WP_041209071.1 YggT family protein -
  JUNP479_RS09585 (JUNP479_1948) yggU 2132347..2132646 (+) 300 WP_033114039.1 DUF167 family protein YggU -
  JUNP479_RS09590 (JUNP479_1949) - 2132676..2133095 (+) 420 WP_203977572.1 DUF4426 domain-containing protein -
  JUNP479_RS09595 (JUNP479_1950) - 2133198..2134394 (-) 1197 WP_104016015.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=85239 JUNP479_RS09565 WP_005340354.1 2129115..2130149(-) (pilT) [Aeromonas jandaei strain JUNP479]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=85239 JUNP479_RS09565 WP_005340354.1 2129115..2130149(-) (pilT) [Aeromonas jandaei strain JUNP479]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCGTCGGATCTACACCTGTCCGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACATCGGGAAGTGCACGCCCTCATTTACGACA
TCATGAACGATCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCTTTCCAGCAAGCGCGTGGTTCAGGCGCTGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGCGGTCTGGTACTGGTGACCGGGCCGACCGGTT
CCGGTAAGTCGACCACGCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATTCTCACCATCGAAGAC
CCCATCGAATTTGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
TGCCCTGCGCTCGGCACTGCGGGAAGACCCCGACATCATTCTGGTGGGTGAGATGCGCGATCTTGAAACCATTCGCCTCG
CCATGACCGCAGCCGAAACCGGCCACTTGGTATTTGGCACCCTGCACACCTCGTCGGCAGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCAGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGCGGTCGGGTGGCAGCTCACGAGATCATGATGGGGATCCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGCGGCGTGGTGGCATCTCTCGATGCCAAGGCCAAGGCGGTTGACCCCAACAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment