Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JUNP479_RS09560 Genome accession   NZ_AP024466
Coordinates   2127977..2129086 (-) Length   369 a.a.
NCBI ID   WP_203977777.1    Uniprot ID   -
Organism   Aeromonas jandaei strain JUNP479     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2122977..2134086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JUNP479_RS09530 (JUNP479_1936) - 2123548..2124555 (-) 1008 WP_203977776.1 Fe(3+) ABC transporter substrate-binding protein -
  JUNP479_RS09535 (JUNP479_1937) glnK 2124749..2125087 (-) 339 WP_033114048.1 P-II family nitrogen regulator -
  JUNP479_RS09540 (JUNP479_1938) arfB 2125289..2125705 (+) 417 WP_033114047.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  JUNP479_RS09545 (JUNP479_1939) - 2125727..2126218 (+) 492 WP_203977566.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  JUNP479_RS09550 (JUNP479_1940) - 2126533..2126961 (-) 429 WP_033114045.1 hypothetical protein -
  JUNP479_RS09555 (JUNP479_1941) yaaA 2127156..2127929 (-) 774 WP_203977568.1 peroxide stress protein YaaA -
  JUNP479_RS09560 (JUNP479_1942) pilU 2127977..2129086 (-) 1110 WP_203977777.1 type IVa pilus ATPase TapU Machinery gene
  JUNP479_RS09565 (JUNP479_1943) pilT 2129115..2130149 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  JUNP479_RS09570 (JUNP479_1945) - 2130189..2130890 (+) 702 WP_041209073.1 YggS family pyridoxal phosphate-dependent enzyme -
  JUNP479_RS09575 (JUNP479_1946) proC 2130957..2131784 (+) 828 WP_203977570.1 pyrroline-5-carboxylate reductase -
  JUNP479_RS09580 (JUNP479_1947) - 2131796..2132347 (+) 552 WP_041209071.1 YggT family protein -
  JUNP479_RS09585 (JUNP479_1948) yggU 2132347..2132646 (+) 300 WP_033114039.1 DUF167 family protein YggU -
  JUNP479_RS09590 (JUNP479_1949) - 2132676..2133095 (+) 420 WP_203977572.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41128.22 Da        Isoelectric Point: 6.4419

>NTDB_id=85238 JUNP479_RS09560 WP_203977777.1 2127977..2129086(-) (pilU) [Aeromonas jandaei strain JUNP479]
MNLDELLGQLVEKKGSDLFVTVGTPPTLKVNGHLLSLGGEVLDKKAALMLVRETLSPDHFERYIRTREANYAIHRETLGR
FRVSAFWQQELPGMVVRRIETRIPTFDDLQLPVILQEVALAKRGLVLFVGATGAGKSTTQATMIGYRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSSETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLKAIVAQQLVPSVDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQS
LFNLFCSGQIGYSEALAHADSANDLRLLIKLSGRDQLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=85238 JUNP479_RS09560 WP_203977777.1 2127977..2129086(-) (pilU) [Aeromonas jandaei strain JUNP479]
ATGAATCTGGATGAACTGTTGGGCCAGCTGGTGGAGAAAAAAGGGTCGGATCTCTTTGTGACGGTGGGGACGCCCCCCAC
CCTCAAGGTGAACGGCCATCTGCTCTCTTTGGGGGGCGAAGTGCTCGATAAAAAGGCTGCTCTCATGCTGGTGAGGGAGA
CCCTGAGCCCCGACCATTTCGAGCGCTATATCCGCACCAGGGAGGCCAACTACGCCATTCACCGCGAAACTTTGGGTCGG
TTTCGGGTCAGCGCTTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGGATCCCTACCTTCGA
TGACCTGCAACTGCCCGTGATCCTGCAGGAGGTCGCATTGGCCAAGCGCGGACTGGTGCTGTTTGTCGGCGCCACTGGGG
CGGGCAAATCGACCACCCAGGCGACCATGATTGGTTATCGCAACCAGCATGCCGACGGCCATATTCTGACGGTGGAAGAT
CCGGTGGAGTTTGTCCATCAGCATGGCCGCAGCCTGATCACCCAGCGGGAGGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGTTGAAAAGCTCGCTGCGTCAGGCGCCGGACGTCATTCTGATCGGCGAGATCAGAAGCTCGGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACCGGCCACCTCTGCCTCGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATT
CTGCATCTGGTGCCGCAGGAGAAGCATCGTCAGTTCCTGTTCGATCTCTCCTTCAACCTCAAGGCCATTGTCGCCCAGCA
ACTGGTGCCAAGCGTGGATGGCAAGCGGCGCTGCGCCGCATTCGAGATCCTGCTCAACACCCCGCTTATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAATCGACCGAGCTTGGCATGCAAACCTTCGATCAATCG
CTCTTCAACCTGTTCTGCTCCGGCCAGATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTTTCCGGCCGTGATCAGCTTGGAACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.783

99.729

0.596
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.708

91.87

0.374
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment