Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QWG60_RS15580 Genome accession   NZ_CP129121
Coordinates   3309339..3310376 (-) Length   345 a.a.
NCBI ID   WP_107180913.1    Uniprot ID   -
Organism   Halomonas halophila strain CCM 3662     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3304339..3315376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWG60_RS15565 (QWG60_15565) tyrS 3305564..3306763 (-) 1200 WP_046079382.1 tyrosine--tRNA ligase -
  QWG60_RS15570 (QWG60_15570) - 3306875..3308005 (+) 1131 WP_146908806.1 anhydro-N-acetylmuramic acid kinase -
  QWG60_RS15575 (QWG60_15575) pilU 3308170..3309312 (-) 1143 WP_146908808.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QWG60_RS15580 (QWG60_15580) pilT 3309339..3310376 (-) 1038 WP_107180913.1 type IV pilus twitching motility protein PilT Machinery gene
  QWG60_RS15585 (QWG60_15585) - 3310431..3311138 (+) 708 WP_107180914.1 YggS family pyridoxal phosphate-dependent enzyme -
  QWG60_RS15590 (QWG60_15590) proC 3311200..3312024 (+) 825 WP_146908810.1 pyrroline-5-carboxylate reductase -
  QWG60_RS15595 (QWG60_15595) - 3312071..3312664 (+) 594 WP_035593026.1 YggT family protein -
  QWG60_RS15600 (QWG60_15600) - 3312848..3314005 (+) 1158 WP_146908812.1 homoserine O-acetyltransferase -
  QWG60_RS15605 (QWG60_15605) metW 3314005..3314598 (+) 594 WP_035593045.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38237.74 Da        Isoelectric Point: 6.4218

>NTDB_id=851162 QWG60_RS15580 WP_107180913.1 3309339..3310376(-) (pilT) [Halomonas halophila strain CCM 3662]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDDREVRKLIYDILTDRQRRDFEEYLETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPSEVLTMEDLGMGETFRRLAMLPRGLVLVTGPTGSGKSTTLAAMIDHINDNRFEHILTIED
PVEFVHRSKRCLINQREVHRDTQGFAPALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKAMVRSMLSESLQAVISQTLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSALQTGGNLGMQTLDTAL
SRLVADNVVAREEAQTKARATLAHQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=851162 QWG60_RS15580 WP_107180913.1 3309339..3310376(-) (pilT) [Halomonas halophila strain CCM 3662]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAACGCCTCGGACCTTCACCTTTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGACGGCGACATTCGGCGGCTCAACGTGCCGGCCATGGACGATCGTGAGGTCCGCAAGCTGATCTACGATA
TCCTCACCGACCGTCAGCGGCGCGACTTCGAGGAGTACCTGGAAACCGACTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTTCGCGTCAACGTCTTCAACCAGGCCCGCGGCGCCGGGGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGACCATGGA
GGATCTGGGCATGGGCGAGACGTTCCGCCGCCTGGCCATGCTGCCGCGCGGCCTGGTGCTGGTGACCGGGCCTACCGGCT
CGGGCAAGAGCACCACCCTGGCGGCGATGATCGACCACATCAACGACAACCGCTTCGAGCATATCCTGACCATCGAGGAT
CCGGTGGAATTCGTGCATCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCAGGGCTTCGCCCC
GGCGCTGCGCAGTGCGCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAGATGCGCGATCTGGAGACCATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGCCATCTGGTGTTCGGCACCCTGCATACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGATGTCTTCCCGGGGGACGAGAAGGCCATGGTGCGCTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTCAAGCGCCAGGGCGGCGGCCGGGTGGCGGCCCACGAGATCCTGATCGCCACCTCGGCCGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGCTGCAGACCGGCGGCAACCTGGGCATGCAGACCCTGGATACGGCGCTG
TCGCGGCTGGTGGCGGACAACGTGGTCGCCCGTGAGGAAGCCCAGACCAAGGCGCGAGCCACGCTCGCGCATCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.056

98.261

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Acinetobacter baumannii D1279779

76.106

98.261

0.748
  pilT Acinetobacter baumannii strain A118

76.106

98.261

0.748
  pilT Acinetobacter nosocomialis M2

75.811

98.261

0.745
  pilT Acinetobacter baylyi ADP1

75

98.551

0.739
  pilT Legionella pneumophila strain Lp02

69.231

97.971

0.678
  pilT Legionella pneumophila strain ERS1305867

69.231

97.971

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.681

0.667
  pilT Vibrio cholerae strain A1552

68.249

97.681

0.667
  pilT Neisseria meningitidis 8013

66.471

98.551

0.655
  pilT Neisseria gonorrhoeae MS11

66.176

98.551

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.802

98.261

0.519
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Vibrio cholerae strain A1552

39.701

97.101

0.386