Detailed information NTG0851161

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QWG60_RS15575
• Genome accession: NZ_CP129121
• Coordinates: 3308170..3309312 (-)
• Length: 380 a.a.
• NCBI ID: WP_146908808.1
• Uniprot ID: -
• Organism: Halomonas halophila strain CCM 3662
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3303170..3314312

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QWG60_RS15565 (QWG60_15565)	tyrS	3305564..3306763 (-)	1200	WP_046079382.1	tyrosine--tRNA ligase	-
QWG60_RS15570 (QWG60_15570)	-	3306875..3308005 (+)	1131	WP_146908806.1	anhydro-N-acetylmuramic acid kinase	-
QWG60_RS15575 (QWG60_15575)	pilU	3308170..3309312 (-)	1143	WP_146908808.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QWG60_RS15580 (QWG60_15580)	pilT	3309339..3310376 (-)	1038	WP_107180913.1	type IV pilus twitching motility protein PilT	Machinery gene
QWG60_RS15585 (QWG60_15585)	-	3310431..3311138 (+)	708	WP_107180914.1	YggS family pyridoxal phosphate-dependent enzyme	-
QWG60_RS15590 (QWG60_15590)	proC	3311200..3312024 (+)	825	WP_146908810.1	pyrroline-5-carboxylate reductase	-
QWG60_RS15595 (QWG60_15595)	-	3312071..3312664 (+)	594	WP_035593026.1	YggT family protein	-
QWG60_RS15600 (QWG60_15600)	-	3312848..3314005 (+)	1158	WP_146908812.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 41991.01 Da
• Isoelectric Point: 5.4306

>NTDB_id=851161 QWG60_RS15575 WP_146908808.1 3308170..3309312(-) (pilU) [Halomonas halophila strain CCM 3662]
MTAKEWLYQLLDIMIEKEASDLLISVGAPPTLKMAGQLTPMGEEGLSVAQVGELVAAAMPENHRARFDDEQEANFALSLS
GRGRFRVSAFRQRNQPAMVLRRIAFEIPHLEELSLPEVLGELANIKRGLVFVVGGTGTGKSTTLASMIQQRNETAGGHII
SIEDPIEYVHPHRRAIINQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALAFAETGHLCLATLHANNANQA
LERIIHFFPHERHDQVWLDLSLNLQGIVAQQLLPTQAGGRCPAIEVLLKSPLIADLIRKGEVADVKNVMARSRDMGMQTF
DQALYDLYKRGAISEEVALVHADSANDLRMMIKYGDEQGVGLAQAKEAASHLSLRGEDDY

Nucleotide

• Length: 1143 bp

>NTDB_id=851161 QWG60_RS15575 WP_146908808.1 3308170..3309312(-) (pilU) [Halomonas halophila strain CCM 3662]
ATGACCGCGAAGGAATGGCTGTATCAGCTGCTCGACATCATGATCGAGAAGGAAGCCTCGGACCTGCTGATCTCGGTGGG
CGCGCCGCCGACGCTGAAGATGGCCGGTCAGCTGACGCCCATGGGCGAAGAAGGGCTGTCGGTGGCGCAGGTCGGCGAGC
TGGTGGCGGCGGCAATGCCCGAGAATCACCGGGCGCGCTTCGACGATGAGCAGGAGGCCAATTTCGCGCTCAGCCTGTCG
GGGCGGGGGCGCTTCCGCGTCAGTGCCTTCCGCCAGCGTAACCAGCCGGCCATGGTGCTCCGTCGCATCGCCTTCGAGAT
TCCCCATCTCGAGGAACTGTCGCTGCCGGAGGTGCTCGGCGAGCTGGCCAACATCAAGCGCGGGCTGGTGTTCGTGGTCG
GCGGCACCGGCACCGGCAAGTCGACGACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCGCCGGCGGCCACATCATC
AGCATCGAGGATCCGATCGAGTACGTGCACCCCCACCGCCGAGCGATCATCAACCAGCGCGAGGTCGGCATCGACACCGA
GTCGTTCGAGGTGGCGCTAAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAACATGCGCTGGCTTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCATGCCAACAACGCCAACCAGGCG
CTGGAACGCATCATCCACTTCTTCCCCCACGAGCGGCACGACCAGGTCTGGCTCGACCTGTCGCTCAACCTGCAGGGCAT
CGTCGCCCAGCAGCTGCTGCCGACCCAGGCCGGTGGCCGCTGCCCGGCCATCGAGGTCCTGCTGAAGTCGCCGCTGATCG
CCGACCTGATCCGCAAGGGGGAGGTGGCGGACGTCAAGAACGTCATGGCCCGCTCCCGGGACATGGGCATGCAGACCTTC
GACCAGGCGCTCTATGACCTCTACAAGAGGGGGGCGATCAGCGAAGAGGTCGCGCTGGTGCATGCCGACTCGGCCAACGA
TCTGCGCATGATGATCAAGTACGGCGACGAGCAGGGGGTGGGGCTGGCGCAGGCCAAGGAGGCGGCCAGTCATCTGTCCC
TGCGCGGCGAGGACGACTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (70-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64.286	92.105	0.592
pilU	Vibrio cholerae strain A1552	60.665	95	0.576
pilU	Acinetobacter baylyi ADP1	57.143	92.105	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.144	96.579	0.397