Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QVG61_RS01440 Genome accession   NZ_CP128988
Coordinates   294671..295708 (+) Length   345 a.a.
NCBI ID   WP_289931538.1    Uniprot ID   -
Organism   Thiohalobacter sp. IOR34     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 289671..300708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVG61_RS01420 (QVG61_01420) - 292119..292397 (-) 279 WP_354671174.1 DUF167 family protein -
  QVG61_RS01425 (QVG61_01425) - 292397..292960 (-) 564 WP_289931535.1 YggT family protein -
  QVG61_RS01430 (QVG61_01430) proC 292961..293788 (-) 828 WP_289931536.1 pyrroline-5-carboxylate reductase -
  QVG61_RS01435 (QVG61_01435) - 293868..294578 (-) 711 WP_289931537.1 YggS family pyridoxal phosphate-dependent enzyme -
  QVG61_RS01440 (QVG61_01440) pilT 294671..295708 (+) 1038 WP_289931538.1 type IV pilus twitching motility protein PilT Machinery gene
  QVG61_RS01445 (QVG61_01445) pilU 295786..296919 (+) 1134 WP_289932676.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QVG61_RS01450 (QVG61_01450) - 296930..297817 (-) 888 WP_289931539.1 dihydroorotate dehydrogenase electron transfer subunit -
  QVG61_RS01455 (QVG61_01455) - 297861..299162 (-) 1302 WP_289931540.1 dihydroorotase -
  QVG61_RS01460 (QVG61_01460) - 299159..300097 (-) 939 Protein_292 aspartate carbamoyltransferase catalytic subunit -
  QVG61_RS01465 (QVG61_01465) pyrR 300127..300633 (-) 507 WP_289931542.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38308.98 Da        Isoelectric Point: 6.5803

>NTDB_id=850214 QVG61_RS01440 WP_289931538.1 294671..295708(+) (pilT) [Thiohalobacter sp. IOR34]
MDIAELLAFGVKNNASDLHLSADLPPMIRVDGDIRRINVPALDHKQVHALVYDIMNDKQRKDFEEFLETDFSFEIPNLAR
FRVNAFNHNRGAGAVFRTIPSKVLTLEELGAPAVFKEIADQPRGIVLVTGPTGSGKSTTLAAMMNHVNETRFEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKTGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGQAVGMQTLDQNL
QDLVQRGLISRLDARSKAANKELFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=850214 QVG61_RS01440 WP_289931538.1 294671..295708(+) (pilT) [Thiohalobacter sp. IOR34]
ATGGATATCGCAGAGCTTCTCGCCTTCGGCGTCAAGAATAACGCCTCCGACCTGCATCTGTCTGCAGATCTGCCGCCGAT
GATCCGCGTCGATGGCGACATCCGCCGCATCAACGTGCCGGCGCTGGACCACAAGCAGGTCCATGCGCTGGTCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTGGAGACCGACTTCTCCTTCGAGATCCCCAACCTGGCGCGT
TTCCGCGTCAATGCCTTCAACCACAACCGCGGTGCCGGCGCGGTGTTCCGTACCATCCCCTCCAAGGTGCTGACCCTGGA
AGAGCTGGGGGCGCCGGCGGTATTCAAGGAGATCGCCGACCAGCCGCGCGGCATCGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATGAATCATGTCAACGAGACCCGCTTCGAGCACATCCTGACCATCGAGGAT
CCGATCGAGTTCGTGCATGAATCCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCCCTGCGATCGGCGTTGCGCGAGGATCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CATTGACCGCGGCCGAGACCGGCCATCTGGTGTTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTTCCCGCAGCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAGTCGCTGCGTGCGGTCATCTCCCAGAC
CCTGCTGAAGAAGACCGGCGGCGGGCGCATCGCGGCCCATGAGATCATGATCGGCACCCCGGCGATTCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCCGCCATCCAGACCGGCCAGGCGGTGGGCATGCAGACCCTGGACCAGAACCTG
CAGGATCTGGTCCAACGGGGGCTGATCAGTCGTCTCGATGCGCGCAGCAAGGCGGCCAACAAGGAGCTGTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Acinetobacter baumannii D1279779

81.105

99.71

0.809
  pilT Acinetobacter baumannii strain A118

81.105

99.71

0.809
  pilT Acinetobacter nosocomialis M2

81.105

99.71

0.809
  pilT Acinetobacter baylyi ADP1

79.07

99.71

0.788
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.681

0.696
  pilT Vibrio cholerae strain A1552

71.217

97.681

0.696
  pilT Neisseria meningitidis 8013

69.679

99.42

0.693
  pilT Neisseria gonorrhoeae MS11

69.388

99.42

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Vibrio cholerae strain A1552

42.388

97.101

0.412
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4