Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QVG61_RS01445 Genome accession   NZ_CP128988
Coordinates   295786..296919 (+) Length   377 a.a.
NCBI ID   WP_289932676.1    Uniprot ID   -
Organism   Thiohalobacter sp. IOR34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 290786..301919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVG61_RS01420 (QVG61_01420) - 292119..292397 (-) 279 WP_354671174.1 DUF167 family protein -
  QVG61_RS01425 (QVG61_01425) - 292397..292960 (-) 564 WP_289931535.1 YggT family protein -
  QVG61_RS01430 (QVG61_01430) proC 292961..293788 (-) 828 WP_289931536.1 pyrroline-5-carboxylate reductase -
  QVG61_RS01435 (QVG61_01435) - 293868..294578 (-) 711 WP_289931537.1 YggS family pyridoxal phosphate-dependent enzyme -
  QVG61_RS01440 (QVG61_01440) pilT 294671..295708 (+) 1038 WP_289931538.1 type IV pilus twitching motility protein PilT Machinery gene
  QVG61_RS01445 (QVG61_01445) pilU 295786..296919 (+) 1134 WP_289932676.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QVG61_RS01450 (QVG61_01450) - 296930..297817 (-) 888 WP_289931539.1 dihydroorotate dehydrogenase electron transfer subunit -
  QVG61_RS01455 (QVG61_01455) - 297861..299162 (-) 1302 WP_289931540.1 dihydroorotase -
  QVG61_RS01460 (QVG61_01460) - 299159..300097 (-) 939 Protein_292 aspartate carbamoyltransferase catalytic subunit -
  QVG61_RS01465 (QVG61_01465) pyrR 300127..300633 (-) 507 WP_289931542.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  QVG61_RS01470 (QVG61_01470) ruvX 300638..301066 (-) 429 WP_289931543.1 Holliday junction resolvase RuvX -
  QVG61_RS01475 (QVG61_01475) - 301059..301622 (-) 564 WP_289931544.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42134.26 Da        Isoelectric Point: 6.4433

>NTDB_id=850215 QVG61_RS01445 WP_289932676.1 295786..296919(+) (pilU) [Thiohalobacter sp. IOR34]
MELNTLLRVMVEKKASDLFITAGIPPSVKIDGRIRPVSKDPLSPEQAREMVYSAMSPTQRNEFDACHECNFAISASGLGR
FRVSAFFQRNHIGMVLRRIETEIPSIESLHLPPIIKQLALLKRGLVIFAGATGTGKSTSLASMIGYRNRHGDGHIVTLED
PIEFIHQHDGCIITQREVGLDTESYEVGLKNTLRQAPDVILLGEIRTRETMDYAIAFAETGHLCLSTLHANNANQALDRI
INFFPEDRRDQLLMDLSLNLKAIVAQQLIPGADGKGRRAAVEVLMNTPLAAEHIRHGEIYKLKDLMRRSNQSGMKTFDQA
LYDLYKAGEISYEDALHHADSPNEVRLMIKLDEEDPAKLADAIGNLTLEDADKPYRR

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=850215 QVG61_RS01445 WP_289932676.1 295786..296919(+) (pilU) [Thiohalobacter sp. IOR34]
ATGGAACTCAACACCCTGTTGCGGGTGATGGTGGAGAAGAAGGCATCGGATCTGTTCATCACCGCCGGTATTCCTCCCAG
CGTCAAGATCGACGGCCGGATCCGCCCGGTCAGCAAGGATCCGCTCAGTCCCGAGCAGGCGCGGGAGATGGTCTACAGCG
CCATGTCACCCACCCAGCGCAACGAGTTCGACGCCTGCCACGAGTGCAACTTCGCCATCAGCGCCAGCGGTCTGGGGCGG
TTCCGGGTCAGCGCCTTCTTCCAGCGCAACCACATCGGTATGGTGCTGCGGCGCATCGAGACCGAGATTCCCAGCATCGA
GTCGCTGCATCTGCCACCGATCATCAAGCAGCTGGCGCTGCTCAAGCGCGGCCTGGTGATCTTCGCCGGCGCCACCGGCA
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACCGCCACGGGGACGGGCACATCGTCACCCTGGAAGAC
CCCATCGAGTTCATCCACCAGCATGACGGCTGCATCATCACCCAGCGCGAGGTGGGTCTGGACACCGAGTCCTACGAGGT
GGGGCTCAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTGCTCGGCGAGATCCGCACCCGCGAGACCATGGACTATG
CCATCGCCTTCGCCGAGACCGGTCACCTCTGCCTCTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGAGGACCGCCGCGACCAGTTGCTGATGGATCTGTCGCTGAACCTCAAGGCGATCGTCGCCCAGCA
GCTGATTCCGGGAGCCGACGGCAAGGGCCGGCGTGCCGCGGTGGAGGTGCTGATGAACACCCCGCTGGCGGCCGAGCACA
TCCGTCATGGTGAGATCTACAAGCTGAAGGACCTGATGCGCCGCTCCAACCAGAGTGGCATGAAGACCTTCGACCAGGCG
CTGTACGACCTGTACAAGGCCGGTGAGATCAGCTACGAGGATGCCCTGCACCATGCCGATTCGCCGAACGAGGTACGGCT
GATGATCAAGCTCGACGAGGAAGATCCGGCGAAGCTGGCCGATGCCATCGGCAACCTGACCCTGGAGGACGCGGACAAGC
CCTACCGGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

70.777

98.939

0.7
  pilU Acinetobacter baylyi ADP1

66.479

94.164

0.626
  pilU Vibrio cholerae strain A1552

56.901

94.164

0.536
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.86

91.247

0.382
  pilT Legionella pneumophila strain Lp02

41.791

88.859

0.371
  pilT Legionella pneumophila strain ERS1305867

41.791

88.859

0.371